Expression, purification and characterization of recombinant human proinsulin

We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromato...

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Veröffentlicht in:FEBS letters 1997-02, Vol.402 (2), p.124-130
Hauptverfasser: Cowley, Darrin J, Mackin, Robert B
Format: Artikel
Sprache:eng
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