Detection of anti‐liver cell membrane antibody using a human hepatocellular carcinoma cell line

A radioimmunometric technique for the detection of autoantibodies to liver membrane antigens has been developed using Alexander cells, a human hepatocellular carcinoma cell line. After incubation of Alexander cells with serum, antimembrane antibodies were detected by addition of 125I‐labeled Protein...

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Veröffentlicht in:	Hepatol.; (United States) 1989-02, Vol.9 (2), p.210-214
Hauptverfasser:	Lobo‐Yeo, Ava, McSorley, Claire, McFarlane, Barbara M., Mieli‐Vergani, Giorgina, Mowat, Alex P., Vergani, Diego
Format:	Artikel
Sprache:	eng
Schlagworte:	550901 - Pathology- Tracer Techniques Adolescent AGE GROUPS alpha 1-Antitrypsin Deficiency ANIMAL CELLS ANTIBODIES ANTIGEN-ANTIBODY REACTIONS ANTIGENS Autoantibodies - analysis Autoimmune Diseases - immunology BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES Biological and medical sciences BODY Carcinoma, Hepatocellular - immunology CARCINOMAS CELL CONSTITUENTS Cell Membrane - immunology CELL MEMBRANES Child Child, Preschool CHILDREN Cholangitis, Sclerosing - immunology DAYS LIVING RADIOISOTOPES DIGESTIVE SYSTEM DIGESTIVE SYSTEM DISEASES DISEASES ELECTRON CAPTURE RADIOISOTOPES Gastroenterology. Liver. Pancreas. Abdomen GLANDS HEPATITIS Hepatitis, Chronic - immunology Hepatolenticular Degeneration - immunology Humans IMMUNOASSAY IMMUNOLOGY Immunosorbent Techniques Infant INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES Iodine Radioisotopes ISOTOPE APPLICATIONS ISOTOPES LIVER Liver - immunology Liver Diseases - immunology Liver Neoplasms - immunology Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences MEMBRANES NEOPLASMS NUCLEI ODD-EVEN NUCLEI ORGANS PATHOGENESIS RADIOASSAY RADIOIMMUNOASSAY RADIOIMMUNOLOGY RADIOISOTOPES RHEUMATIC DISEASES SKELETAL DISEASES Staphylococcal Protein A TRACER TECHNIQUES TUMOR CELLS Tumor Cells, Cultured Tumors
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