Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution
A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tiss...
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| Veröffentlicht in: | Journal of Chromatography A 1997-01, Vol.758 (2), p.313-318 |
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| container_end_page | 318 |
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| container_issue | 2 |
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| container_title | Journal of Chromatography A |
| container_volume | 758 |
| creator | Açil, Yahya Brinckmann, Jürgen Behrens, Peter Müller, Peter K. Bätge, Boris |
| description | A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g. chromatographic procedures. |
| doi_str_mv | 10.1016/S0021-9673(96)00729-7 |
| format | Article |
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The procedure involves sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. 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The procedure involves sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g. chromatographic procedures.</description><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Collagen - chemistry</subject><subject>Collagen - isolation & purification</subject><subject>Collagens</subject><subject>Electroelution</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>Hydroxylysine - analysis</subject><subject>Hydroxyproline - analysis</subject><subject>Pepsin A - metabolism</subject><subject>Proteins</subject><subject>Sodium Dodecyl Sulfate - chemistry</subject><subject>Swine</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu1TAQhr0AtaXwCJW8QAgkQh07F3uFqorLkSqxKHRrOeNJMXLiYCeV8g59aHzOic6WhcfW_P_MeD5Crkr2qWRlc33PGC8L1bTivWo-MNZyVbQvyMUpfU5epfSHsbLN2hk5U6zirZAX5PkeBzdFnEw0s3tC6lLw-RVGGnoKwXvziCOd1wkT3X2ku8PZUTNa-kC7laZg3TJQGyzC6mlafG9mLKbgVwNx9WZwFukjeooeYY5h-h0iJpcOLbYc-mU_8jV52Ruf8M12X5JfX7_8vP1e3P34tru9uStASDUXUjRcAdSdAC5A1KrphBGNVTV2nZJtZyvkVa-wr2RjjUGmoAIpQHa8xkqIS_Lu2HeK4e-CadaDS4B51xHDknQrJReCsWysj0aIIaWIvZ6iG0xcdcn0nrw-kNd7xDnoA3nd5rqrbcDSDWhPVRv2rL_ddJPA-D6aEVw62XjdyKrk2fb5aMMM48lh1AkcjoDWxcxN2-D-85F_lyikhw</recordid><startdate>19970117</startdate><enddate>19970117</enddate><creator>Açil, Yahya</creator><creator>Brinckmann, Jürgen</creator><creator>Behrens, Peter</creator><creator>Müller, Peter K.</creator><creator>Bätge, Boris</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970117</creationdate><title>Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution</title><author>Açil, Yahya ; Brinckmann, Jürgen ; Behrens, Peter ; Müller, Peter K. ; Bätge, Boris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-83629cc5b3c23c3596b3a36d95ebb987bd4e24f9ef486daae09c4c83c8b25e433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Collagen - chemistry</topic><topic>Collagen - isolation & purification</topic><topic>Collagens</topic><topic>Electroelution</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins</topic><topic>Hydroxylysine - analysis</topic><topic>Hydroxyproline - analysis</topic><topic>Pepsin A - metabolism</topic><topic>Proteins</topic><topic>Sodium Dodecyl Sulfate - chemistry</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Açil, Yahya</creatorcontrib><creatorcontrib>Brinckmann, Jürgen</creatorcontrib><creatorcontrib>Behrens, Peter</creatorcontrib><creatorcontrib>Müller, Peter K.</creatorcontrib><creatorcontrib>Bätge, Boris</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Açil, Yahya</au><au>Brinckmann, Jürgen</au><au>Behrens, Peter</au><au>Müller, Peter K.</au><au>Bätge, Boris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1997-01-17</date><risdate>1997</risdate><volume>758</volume><issue>2</issue><spage>313</spage><epage>318</epage><pages>313-318</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A simple method for the isolation of α-chains of different collagen types was developed. 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| subjects | Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Collagen - chemistry Collagen - isolation & purification Collagens Electroelution Electrophoresis, Polyacrylamide Gel - methods Fundamental and applied biological sciences. Psychology Glycoproteins Hydroxylysine - analysis Hydroxyproline - analysis Pepsin A - metabolism Proteins Sodium Dodecyl Sulfate - chemistry Swine |
| title | Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution |
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