Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves
This research was designed to improve our understanding of oocyte maturation and acquisition of developmental competence of oocytes using prepubertal heifers as a model. Oocytes were collected by ultrasound-guided transvaginal oocyte retrieval from 20 age-matched calves at 5, 7, 9, and 11 mo of age...
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| Veröffentlicht in: | Biology of reproduction 1997-02, Vol.56 (2), p.386-392 |
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| creator | Presicce, G.A. (Cornell University, Ithaca, NY.) Jiang, S Simkin, M Zhang, L Looney, C.R Godke, R.A Yang, X.Z |
| description | This research was designed to improve our understanding of oocyte maturation and acquisition of developmental competence of oocytes using prepubertal heifers as a model. Oocytes were collected by ultrasound-guided transvaginal oocyte retrieval from 20 age-matched calves at 5, 7, 9, and 11 mo of age that had or had not received gonadotropin stimulation. Numbers of oocytes recovered from unstimulated heifers decreased with age, being 15 +/- 2, 12 +/- 1, 7 +/- 1, and 7 +/- 1 (for 5-, 7-, 9-, and 11-mo-old calves, respectively). Corresponding numbers for the gonadotropin-stimulated heifers were 18 +/- 2, 16 +/- 2, 13 +/- 1, and 15 +/- 2. Cumulus-oocyte complexes were graded from A to D on the basis of their morphology, and the better-grade A and B oocytes were used for in vitro maturation and fertilization. A higher proportion of grade A and B oocytes was found for the stimulated vs. unstimulated prepubertal calves at 5 mo of age (92% vs. 49%, p 0.05) and 7 mo of age (96% vs. 63%, p 0.05), but the improvement of this parameter by stimulation was not significant for peri- and postpubertal calves at 9 (53% vs. 38%, p 0.05) and 11 (53% vs. 38%, p 0.05) mo of age. Embryo development to morula and blastocyst stages was poorer (0-11%, p 0.05) for oocytes collected from unstimulated calves at 5-9 mo of age than for those from the age-matched but gonadotropin-stimulated groups (10%, 39%, and 31%, at 5, 7, and 9 mo of age, respectively). In calves 11 mo of age, embryo development to morula and blastocyst stages was similar with and without gonadotropin stimulation (48% vs. 40%, p 0.05) and was comparable to that of adult cow oocytes (45% p 0.05). The data suggest that the acquisition of oocyte competence for normal embryo development in prepubertal calves is influenced by animal age and hormonal treatment |
| doi_str_mv | 10.1095/biolreprod56.2.386 |
| format | Article |
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(Cornell University, Ithaca, NY.) ; Jiang, S ; Simkin, M ; Zhang, L ; Looney, C.R ; Godke, R.A ; Yang, X.Z</creator><creatorcontrib>Presicce, G.A. (Cornell University, Ithaca, NY.) ; Jiang, S ; Simkin, M ; Zhang, L ; Looney, C.R ; Godke, R.A ; Yang, X.Z</creatorcontrib><description>This research was designed to improve our understanding of oocyte maturation and acquisition of developmental competence of oocytes using prepubertal heifers as a model. Oocytes were collected by ultrasound-guided transvaginal oocyte retrieval from 20 age-matched calves at 5, 7, 9, and 11 mo of age that had or had not received gonadotropin stimulation. Numbers of oocytes recovered from unstimulated heifers decreased with age, being 15 +/- 2, 12 +/- 1, 7 +/- 1, and 7 +/- 1 (for 5-, 7-, 9-, and 11-mo-old calves, respectively). Corresponding numbers for the gonadotropin-stimulated heifers were 18 +/- 2, 16 +/- 2, 13 +/- 1, and 15 +/- 2. Cumulus-oocyte complexes were graded from A to D on the basis of their morphology, and the better-grade A and B oocytes were used for in vitro maturation and fertilization. A higher proportion of grade A and B oocytes was found for the stimulated vs. unstimulated prepubertal calves at 5 mo of age (92% vs. 49%, p 0.05) and 7 mo of age (96% vs. 63%, p 0.05), but the improvement of this parameter by stimulation was not significant for peri- and postpubertal calves at 9 (53% vs. 38%, p 0.05) and 11 (53% vs. 38%, p 0.05) mo of age. Embryo development to morula and blastocyst stages was poorer (0-11%, p 0.05) for oocytes collected from unstimulated calves at 5-9 mo of age than for those from the age-matched but gonadotropin-stimulated groups (10%, 39%, and 31%, at 5, 7, and 9 mo of age, respectively). In calves 11 mo of age, embryo development to morula and blastocyst stages was similar with and without gonadotropin stimulation (48% vs. 40%, p 0.05) and was comparable to that of adult cow oocytes (45% p 0.05). The data suggest that the acquisition of oocyte competence for normal embryo development in prepubertal calves is influenced by animal age and hormonal treatment</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod56.2.386</identifier><identifier>PMID: 9116137</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>AGE ; Aging ; Animals ; Biological and medical sciences ; Blastocyst - physiology ; Cattle - physiology ; Chorionic Gonadotropin - pharmacology ; CONTROL HORMONAL ; CONTROLE HORMONAL ; CULTIVO DE EMBRIONES ; CULTURE D'EMBRYON ; DESARROLLO EMBRIONARIO ; DEVELOPPEMENT EMBRYONNAIRE ; EDAD ; Embryonic and Fetal Development ; EXPERIMENTACION IN VITRO ; EXPERIMENTATION IN VITRO ; FECONDATION ; FECUNDACION ; Female ; FEMELLE ; Fertilization in Vitro ; Follicle Stimulating Hormone - pharmacology ; FSH ; Fundamental and applied biological sciences. Psychology ; GONADOTROPHINE ; GONADOTROPINAS ; HEMBRA ; HFS ; MADURACION ; MATURATION ; Morula - physiology ; Oocytes - cytology ; Oocytes - physiology ; Ovarian Follicle - physiology ; Ovulation - physiology ; OVULE ; OVULO ; Pregnancy ; Sexual differentiation and maturation. Puberty. Climacterium ; Sexual Maturation ; TERNERO ; VEAU ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1997-02, Vol.56 (2), p.386-392</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>310,311,315,781,785,790,791,23935,23936,25145,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2559643$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9116137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Presicce, G.A. (Cornell University, Ithaca, NY.)</creatorcontrib><creatorcontrib>Jiang, S</creatorcontrib><creatorcontrib>Simkin, M</creatorcontrib><creatorcontrib>Zhang, L</creatorcontrib><creatorcontrib>Looney, C.R</creatorcontrib><creatorcontrib>Godke, R.A</creatorcontrib><creatorcontrib>Yang, X.Z</creatorcontrib><title>Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>This research was designed to improve our understanding of oocyte maturation and acquisition of developmental competence of oocytes using prepubertal heifers as a model. Oocytes were collected by ultrasound-guided transvaginal oocyte retrieval from 20 age-matched calves at 5, 7, 9, and 11 mo of age that had or had not received gonadotropin stimulation. Numbers of oocytes recovered from unstimulated heifers decreased with age, being 15 +/- 2, 12 +/- 1, 7 +/- 1, and 7 +/- 1 (for 5-, 7-, 9-, and 11-mo-old calves, respectively). Corresponding numbers for the gonadotropin-stimulated heifers were 18 +/- 2, 16 +/- 2, 13 +/- 1, and 15 +/- 2. Cumulus-oocyte complexes were graded from A to D on the basis of their morphology, and the better-grade A and B oocytes were used for in vitro maturation and fertilization. A higher proportion of grade A and B oocytes was found for the stimulated vs. unstimulated prepubertal calves at 5 mo of age (92% vs. 49%, p 0.05) and 7 mo of age (96% vs. 63%, p 0.05), but the improvement of this parameter by stimulation was not significant for peri- and postpubertal calves at 9 (53% vs. 38%, p 0.05) and 11 (53% vs. 38%, p 0.05) mo of age. Embryo development to morula and blastocyst stages was poorer (0-11%, p 0.05) for oocytes collected from unstimulated calves at 5-9 mo of age than for those from the age-matched but gonadotropin-stimulated groups (10%, 39%, and 31%, at 5, 7, and 9 mo of age, respectively). In calves 11 mo of age, embryo development to morula and blastocyst stages was similar with and without gonadotropin stimulation (48% vs. 40%, p 0.05) and was comparable to that of adult cow oocytes (45% p 0.05). The data suggest that the acquisition of oocyte competence for normal embryo development in prepubertal calves is influenced by animal age and hormonal treatment</description><subject>AGE</subject><subject>Aging</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - physiology</subject><subject>Cattle - physiology</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>CONTROL HORMONAL</subject><subject>CONTROLE HORMONAL</subject><subject>CULTIVO DE EMBRIONES</subject><subject>CULTURE D'EMBRYON</subject><subject>DESARROLLO EMBRIONARIO</subject><subject>DEVELOPPEMENT EMBRYONNAIRE</subject><subject>EDAD</subject><subject>Embryonic and Fetal Development</subject><subject>EXPERIMENTACION IN VITRO</subject><subject>EXPERIMENTATION IN VITRO</subject><subject>FECONDATION</subject><subject>FECUNDACION</subject><subject>Female</subject><subject>FEMELLE</subject><subject>Fertilization in Vitro</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>FSH</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GONADOTROPHINE</subject><subject>GONADOTROPINAS</subject><subject>HEMBRA</subject><subject>HFS</subject><subject>MADURACION</subject><subject>MATURATION</subject><subject>Morula - physiology</subject><subject>Oocytes - cytology</subject><subject>Oocytes - physiology</subject><subject>Ovarian Follicle - physiology</subject><subject>Ovulation - physiology</subject><subject>OVULE</subject><subject>OVULO</subject><subject>Pregnancy</subject><subject>Sexual differentiation and maturation. Puberty. Climacterium</subject><subject>Sexual Maturation</subject><subject>TERNERO</subject><subject>VEAU</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUtr3DAQgEVpSbdp_0CgoEPbm7eSZcnSMYS-INBDm7PQY-RVsS1HsrPsv6_SXcochmE-PuaB0A0le0oU_2xjGjMsOXku9u2eSfEC7ShvVdO3Qr5EO0KIaBgT7DV6U8ofQmjHWnaFrhSlgrJ-h4bbAbCZPT6kPKXZjNjDArOH2QFOARv3uMUS15jm5zIld1oBuzQtsP5jQsoYJptPaYAZSiw4znipU20W8lp9zoxPUN6iV8GMBd5d8jV6-Prl99335v7ntx93t_dNaGW3NqCkDxDqmDWcMs56JnsHPQ3MdNYx6UInnJdMkdY7GRwH0jvKCbFgrWLX6NPZW6_yuEFZ9RSLg3E0M6St6F5KSrlgFXx_ATc7gddLjpPJJ325TO1_uPRNqSuEbGYXy3-s5VyJ7lnz8Ywd4nA4xgy6TGYcq5Tp4_HIhW51_Uvlbs5cMEmbIVfVwy_Vd1wpzv4CYiGMVw</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Presicce, G.A. (Cornell University, Ithaca, NY.)</creator><creator>Jiang, S</creator><creator>Simkin, M</creator><creator>Zhang, L</creator><creator>Looney, C.R</creator><creator>Godke, R.A</creator><creator>Yang, X.Z</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves</title><author>Presicce, G.A. (Cornell University, Ithaca, NY.) ; Jiang, S ; Simkin, M ; Zhang, L ; Looney, C.R ; Godke, R.A ; Yang, X.Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f284t-e98dfef143434c9acbd387ce71f3a4bc38cf46cd83902dc8fc5e07c1500bebb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>AGE</topic><topic>Aging</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - physiology</topic><topic>Cattle - physiology</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>CONTROL HORMONAL</topic><topic>CONTROLE HORMONAL</topic><topic>CULTIVO DE EMBRIONES</topic><topic>CULTURE D'EMBRYON</topic><topic>DESARROLLO EMBRIONARIO</topic><topic>DEVELOPPEMENT EMBRYONNAIRE</topic><topic>EDAD</topic><topic>Embryonic and Fetal Development</topic><topic>EXPERIMENTACION IN VITRO</topic><topic>EXPERIMENTATION IN VITRO</topic><topic>FECONDATION</topic><topic>FECUNDACION</topic><topic>Female</topic><topic>FEMELLE</topic><topic>Fertilization in Vitro</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>FSH</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GONADOTROPHINE</topic><topic>GONADOTROPINAS</topic><topic>HEMBRA</topic><topic>HFS</topic><topic>MADURACION</topic><topic>MATURATION</topic><topic>Morula - physiology</topic><topic>Oocytes - cytology</topic><topic>Oocytes - physiology</topic><topic>Ovarian Follicle - physiology</topic><topic>Ovulation - physiology</topic><topic>OVULE</topic><topic>OVULO</topic><topic>Pregnancy</topic><topic>Sexual differentiation and maturation. Puberty. Climacterium</topic><topic>Sexual Maturation</topic><topic>TERNERO</topic><topic>VEAU</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Presicce, G.A. (Cornell University, Ithaca, NY.)</creatorcontrib><creatorcontrib>Jiang, S</creatorcontrib><creatorcontrib>Simkin, M</creatorcontrib><creatorcontrib>Zhang, L</creatorcontrib><creatorcontrib>Looney, C.R</creatorcontrib><creatorcontrib>Godke, R.A</creatorcontrib><creatorcontrib>Yang, X.Z</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Presicce, G.A. (Cornell University, Ithaca, NY.)</au><au>Jiang, S</au><au>Simkin, M</au><au>Zhang, L</au><au>Looney, C.R</au><au>Godke, R.A</au><au>Yang, X.Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>56</volume><issue>2</issue><spage>386</spage><epage>392</epage><pages>386-392</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>This research was designed to improve our understanding of oocyte maturation and acquisition of developmental competence of oocytes using prepubertal heifers as a model. Oocytes were collected by ultrasound-guided transvaginal oocyte retrieval from 20 age-matched calves at 5, 7, 9, and 11 mo of age that had or had not received gonadotropin stimulation. Numbers of oocytes recovered from unstimulated heifers decreased with age, being 15 +/- 2, 12 +/- 1, 7 +/- 1, and 7 +/- 1 (for 5-, 7-, 9-, and 11-mo-old calves, respectively). Corresponding numbers for the gonadotropin-stimulated heifers were 18 +/- 2, 16 +/- 2, 13 +/- 1, and 15 +/- 2. Cumulus-oocyte complexes were graded from A to D on the basis of their morphology, and the better-grade A and B oocytes were used for in vitro maturation and fertilization. A higher proportion of grade A and B oocytes was found for the stimulated vs. unstimulated prepubertal calves at 5 mo of age (92% vs. 49%, p 0.05) and 7 mo of age (96% vs. 63%, p 0.05), but the improvement of this parameter by stimulation was not significant for peri- and postpubertal calves at 9 (53% vs. 38%, p 0.05) and 11 (53% vs. 38%, p 0.05) mo of age. Embryo development to morula and blastocyst stages was poorer (0-11%, p 0.05) for oocytes collected from unstimulated calves at 5-9 mo of age than for those from the age-matched but gonadotropin-stimulated groups (10%, 39%, and 31%, at 5, 7, and 9 mo of age, respectively). In calves 11 mo of age, embryo development to morula and blastocyst stages was similar with and without gonadotropin stimulation (48% vs. 40%, p 0.05) and was comparable to that of adult cow oocytes (45% p 0.05). The data suggest that the acquisition of oocyte competence for normal embryo development in prepubertal calves is influenced by animal age and hormonal treatment</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9116137</pmid><doi>10.1095/biolreprod56.2.386</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1997-02, Vol.56 (2), p.386-392 |
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| language | eng |
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| source | MEDLINE; Oxford Academic Journals (OUP); EZB Electronic Journals Library |
| subjects | AGE Aging Animals Biological and medical sciences Blastocyst - physiology Cattle - physiology Chorionic Gonadotropin - pharmacology CONTROL HORMONAL CONTROLE HORMONAL CULTIVO DE EMBRIONES CULTURE D'EMBRYON DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE EDAD Embryonic and Fetal Development EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO FECONDATION FECUNDACION Female FEMELLE Fertilization in Vitro Follicle Stimulating Hormone - pharmacology FSH Fundamental and applied biological sciences. Psychology GONADOTROPHINE GONADOTROPINAS HEMBRA HFS MADURACION MATURATION Morula - physiology Oocytes - cytology Oocytes - physiology Ovarian Follicle - physiology Ovulation - physiology OVULE OVULO Pregnancy Sexual differentiation and maturation. Puberty. Climacterium Sexual Maturation TERNERO VEAU Vertebrates: reproduction |
| title | Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves |
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