Isolation of a New Peroxisome-Deficient CHO Cell Mutant Defective in Peroxisome Targeting Signal-1 Receptor

For the study of mechanism of peroxisome biogenesis, we attempted to isolate CHO cell mutants deficient in peroxisome biogenesis. We used as the parent strain a stable CHO transformant of ratPEX2(formerly named peroxisome assembly factor-1) cDNA, to avoid unusually frequent isolation ofPex2mutants....

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Veröffentlicht in:	Biochemical and biophysical research communications 1997-01, Vol.230 (2), p.402-406
Hauptverfasser:	Tsukamoto, Toshiro, Bogaki, Akemi, Okumoto, Kanji, Tateishi, Keita, Fujiki, Yukio, Shimozawa, Nobuyuki, Suzuki, Yasuyuki, Kondo, Naomi, Osumi, Takashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Catalase - metabolism Cell Fusion CHO Cells Cricetinae Digitonin - pharmacology DNA Primers Genetic Complementation Test Humans Membrane Proteins - biosynthesis Microbodies - physiology Microbodies - ultrastructure Mutation Peroxisomal Biogenesis Factor 2 Peroxisome-Targeting Signal 1 Receptor Polymerase Chain Reaction Rats Receptors, Cytoplasmic and Nuclear - biosynthesis Receptors, Cytoplasmic and Nuclear - deficiency Receptors, Cytoplasmic and Nuclear - genetics Recombinant Proteins - biosynthesis Transfection
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