CD18 adhesion blockade decreases bacterial clearance and neutrophil recruitment after intrapulmonary E. coli, but not after S. aureus

Leukocyte emigration in the lung occurs by both CD18‐dependent and ‐independent mechanisms that are stimulus specific. We examined the effect of CD18 blockade (mAb 60.3) on neutrophil (PMN) emigration into, and bacterial clearance from, the lung. After intravenous treatment with either mAb 60.3 or s...

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Veröffentlicht in:Journal of leukocyte biology 1997-02, Vol.61 (2), p.167-172
Hauptverfasser: Ramamoorthy, Chandra, Sasaki, Steve S., Su, Daniel L., Sharar, Sam R., Harlan, John M., Winn, Robert K.
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container_end_page 172
container_issue 2
container_start_page 167
container_title Journal of leukocyte biology
container_volume 61
creator Ramamoorthy, Chandra
Sasaki, Steve S.
Su, Daniel L.
Sharar, Sam R.
Harlan, John M.
Winn, Robert K.
description Leukocyte emigration in the lung occurs by both CD18‐dependent and ‐independent mechanisms that are stimulus specific. We examined the effect of CD18 blockade (mAb 60.3) on neutrophil (PMN) emigration into, and bacterial clearance from, the lung. After intravenous treatment with either mAb 60.3 or saline, rabbits were given an intralobar inoculation with 109 colony‐forming units of either Staphylococcus aureus or Escherichia coli. Four hours after inoculation, lungs were lavaged to assess PMN emigration. CD18 blockade reduced PMN emigration to E. coli by 76% but only 45% to S. aureus. Experiments to determine bacterial recovery from the lungs at 4, 8, and 24 h after inoculation showed that CD18 blockade impaired the early (4 h) clearance of E. coli but not S. aureus. These findings suggest that PMN emigration to intrapulmonary S. aureus is largely CD18‐independent. In contrast, intrapulmonary E. coli elicits CD18‐mediated PMN emigration. CD18 blockade results in impaired clearance of E. coli but not S. aureus from the lung. J. Leukoc. Biol. 61: 167–172; 1997.
doi_str_mv 10.1002/jlb.61.2.167
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source MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals
subjects alveolar macrophage
Animals
Antibodies, Monoclonal - pharmacology
CD18 Antigens - immunology
Cell Adhesion - immunology
Cell Movement - immunology
Escherichia coli
Escherichia coli Infections - immunology
Escherichia coli Infections - microbiology
Leukocyte Count
Lung - microbiology
lung lavage
Neutrophils - immunology
Neutrophils - microbiology
Rabbits
Staphylococcal Infections - immunology
Staphylococcal Infections - microbiology
β2 integrins
title CD18 adhesion blockade decreases bacterial clearance and neutrophil recruitment after intrapulmonary E. coli, but not after S. aureus
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