Isolation of early meiotic recombination genes analogous to S. cerevisiae REC104 from the yeasts S. paradoxus and S. pastorianus
The REC104 gene of Saccharomyces cerevisiae is required to initiate recombination in meiosis. Mutations in REC104 eliminate meiotic recombination and lead to the production of inviable spores. To determine if analogous genes exist in other yeasts, clones that hybridized to a REC104 probe were isolat...
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| Veröffentlicht in: | Current genetics 1997-01, Vol.31 (1), p.7-14 |
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| container_title | Current genetics |
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| creator | Nau, J.J Summers, K.R Galbraith, A.M Bullard, S.A Malone, R.E. (Iowa Univ., Iowa City (USA). Dept. of Biological Sciences) |
| description | The REC104 gene of Saccharomyces cerevisiae is required to initiate recombination in meiosis. Mutations in REC104 eliminate meiotic recombination and lead to the production of inviable spores. To determine if analogous genes exist in other yeasts, clones that hybridized to a REC104 probe were isolated from the yeasts S. paradoxus and S. pastorianus. When transformed into a rec104 strain, the REC104 analogs from these two yeasts restored spore viability and meiotic recombination to the same level as a REC104 gene cloned from S. cerevisiae. Compared to S. cerevisiae, the S. paradoxus gene codes for 79% identical amino acids and has 86% nucleic-acid identity in the promoter region and 84% in the coding region. The S. pastorianus gene codes for 63% identical amino acids and has 59% and 71% identity in the promoter and the coding regions, respectively. |
| doi_str_mv | 10.1007/s002940050170 |
| format | Article |
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Dept. of Biological Sciences)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of early meiotic recombination genes analogous to S. cerevisiae REC104 from the yeasts S. paradoxus and S. pastorianus</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1997-01-01</date><risdate>1997</risdate><volume>31</volume><issue>1</issue><spage>7</spage><epage>14</epage><pages>7-14</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>The REC104 gene of Saccharomyces cerevisiae is required to initiate recombination in meiosis. Mutations in REC104 eliminate meiotic recombination and lead to the production of inviable spores. To determine if analogous genes exist in other yeasts, clones that hybridized to a REC104 probe were isolated from the yeasts S. paradoxus and S. pastorianus. When transformed into a rec104 strain, the REC104 analogs from these two yeasts restored spore viability and meiotic recombination to the same level as a REC104 gene cloned from S. cerevisiae. Compared to S. cerevisiae, the S. paradoxus gene codes for 79% identical amino acids and has 86% nucleic-acid identity in the promoter region and 84% in the coding region. The S. pastorianus gene codes for 63% identical amino acids and has 59% and 71% identity in the promoter and the coding regions, respectively.</abstract><cop>United States</cop><pmid>9000375</pmid><doi>10.1007/s002940050170</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular DNA, Fungal - analysis Fungal Proteins - genetics GENE Gene Expression Regulation, Fungal Gene Library GENES Genetic Complementation Test MEIOSE MEIOSIS Meiosis - genetics Molecular Sequence Data Plasmids Promoter Regions, Genetic RECOMBINACION RECOMBINAISON Recombinases RECOMBINATION Recombination, Genetic Saccharomyces - genetics SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Saccharomyces paradoxus Saccharomyces pastorianus Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Transformation, Genetic |
| title | Isolation of early meiotic recombination genes analogous to S. cerevisiae REC104 from the yeasts S. paradoxus and S. pastorianus |
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