Measurement of Ascorbate and Dehydroascorbate Contents in Biological Fluids
Instabilities of ascorbate and dehydroascorbate throughout sample processing are clearly a significant aspect of quantifing of them. Contents of ascorbate in biological fluids decrease with measurable oxidation occurring within minutes to hours. Similarly, dehydroascorbate disappears with chemical o...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1997-01, Vol.69 (2), p.216-220 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Koshiishi, Ichiro Imanari, Toshio |
| description | Instabilities of ascorbate and dehydroascorbate throughout sample processing are clearly a significant aspect of quantifing of them. Contents of ascorbate in biological fluids decrease with measurable oxidation occurring within minutes to hours. Similarly, dehydroascorbate disappears with chemical or enzymatic degradation within minutes. The half-life of dehydroascorbate in human heparinized plasma was ∼2 min. These results indicated that the amount of dehydroascorbate present in sample solutions is a function of both the oxidation of ascorbate and the degradation of dehydroascorbate during the processing of biological fluids. To quantify ascorbate and dehydroascorbate concentrations in biological fluids including circulating blood plasma and urine, we established a high-performance liquid chromatographic method, which requires no pretreatment of sample solutions. |
| doi_str_mv | 10.1021/ac960704k |
| format | Article |
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Contents of ascorbate in biological fluids decrease with measurable oxidation occurring within minutes to hours. Similarly, dehydroascorbate disappears with chemical or enzymatic degradation within minutes. The half-life of dehydroascorbate in human heparinized plasma was ∼2 min. These results indicated that the amount of dehydroascorbate present in sample solutions is a function of both the oxidation of ascorbate and the degradation of dehydroascorbate during the processing of biological fluids. 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Chem</addtitle><description>Instabilities of ascorbate and dehydroascorbate throughout sample processing are clearly a significant aspect of quantifing of them. Contents of ascorbate in biological fluids decrease with measurable oxidation occurring within minutes to hours. Similarly, dehydroascorbate disappears with chemical or enzymatic degradation within minutes. The half-life of dehydroascorbate in human heparinized plasma was ∼2 min. These results indicated that the amount of dehydroascorbate present in sample solutions is a function of both the oxidation of ascorbate and the degradation of dehydroascorbate during the processing of biological fluids. To quantify ascorbate and dehydroascorbate concentrations in biological fluids including circulating blood plasma and urine, we established a high-performance liquid chromatographic method, which requires no pretreatment of sample solutions.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antioxidants</subject><subject>Ascorbic Acid - analysis</subject><subject>Ascorbic Acid - blood</subject><subject>Ascorbic Acid - urine</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Coenzymes, vitamins</subject><subject>Dehydroascorbic Acid - analysis</subject><subject>Dehydroascorbic Acid - blood</subject><subject>Dehydroascorbic Acid - urine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Other biological molecules</subject><subject>Oxidation</subject><subject>Vitamins</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0E1v1DAQBmALgdql9NAfUClCBYlDYOwktnMsC13ohyhi6dWa2BNIm42LnUjtv8doV0GCk6V5H43GL2NHHN5yEPwd2lqCgvLuCVvwSkAutRZP2QIAilwogH32PMZbAM6Byz22p-ta6bpasIsrwjgF2tAwZr7NTqP1ocGRMhxc9oF-PrrgcR4u_TAmGbNuyN53vvc_Oot9dtZPnYsv2LMW-0iHu_eAfT_7uF5-yi-_rD4vTy9zLKEcc-GUEwU0Aqm06EriZCuS1OhGkEsnWqsanTKpOZGrWt4KgUpaVFSBlMUBe73dex_8r4niaDZdtNT3OJCfolFaKaELSPDlP_DWT2FItxnBk9KlrhN6s0U2-BgDteY-dBsMj4aD-dOumdtN9ni3cGo25Ga5qzPlJ7s8VYZ9G3CwXZyZqEpeKZVYvmVdHOlhjjHcGakKVZn19TfztV6vrs4vbswq-Vdbjzb-_cL_5_0GXpic2w</recordid><startdate>19970115</startdate><enddate>19970115</enddate><creator>Koshiishi, Ichiro</creator><creator>Imanari, Toshio</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19970115</creationdate><title>Measurement of Ascorbate and Dehydroascorbate Contents in Biological Fluids</title><author>Koshiishi, Ichiro ; Imanari, Toshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a404t-2d7d230b2ae4cad4e1ec5e6eb8b2ed011cc7b8e4c681eed5f1f22a76ca7e50663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antioxidants</topic><topic>Ascorbic Acid - analysis</topic><topic>Ascorbic Acid - blood</topic><topic>Ascorbic Acid - urine</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Coenzymes, vitamins</topic><topic>Dehydroascorbic Acid - analysis</topic><topic>Dehydroascorbic Acid - blood</topic><topic>Dehydroascorbic Acid - urine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Other biological molecules</topic><topic>Oxidation</topic><topic>Vitamins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koshiishi, Ichiro</creatorcontrib><creatorcontrib>Imanari, Toshio</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koshiishi, Ichiro</au><au>Imanari, Toshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of Ascorbate and Dehydroascorbate Contents in Biological Fluids</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1997-01-15</date><risdate>1997</risdate><volume>69</volume><issue>2</issue><spage>216</spage><epage>220</epage><pages>216-220</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Instabilities of ascorbate and dehydroascorbate throughout sample processing are clearly a significant aspect of quantifing of them. Contents of ascorbate in biological fluids decrease with measurable oxidation occurring within minutes to hours. Similarly, dehydroascorbate disappears with chemical or enzymatic degradation within minutes. The half-life of dehydroascorbate in human heparinized plasma was ∼2 min. These results indicated that the amount of dehydroascorbate present in sample solutions is a function of both the oxidation of ascorbate and the degradation of dehydroascorbate during the processing of biological fluids. To quantify ascorbate and dehydroascorbate concentrations in biological fluids including circulating blood plasma and urine, we established a high-performance liquid chromatographic method, which requires no pretreatment of sample solutions.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>8997895</pmid><doi>10.1021/ac960704k</doi><tpages>5</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Antioxidants Ascorbic Acid - analysis Ascorbic Acid - blood Ascorbic Acid - urine Biochemistry Biological and medical sciences Chromatography, High Pressure Liquid Coenzymes, vitamins Dehydroascorbic Acid - analysis Dehydroascorbic Acid - blood Dehydroascorbic Acid - urine Fundamental and applied biological sciences. Psychology Humans Other biological molecules Oxidation Vitamins |
| title | Measurement of Ascorbate and Dehydroascorbate Contents in Biological Fluids |
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