Effects of the 7/21 Robertsonian translocation on fertilization rates and preimplantation development of bovine oocytes in vitro
A study was conducted to investigate the effect of the 7/21 Robertsonian translocation on fertilization and subsequent development of bovine oocytes matured in vitro. Semen from Japanese Black bulls, 2 with a normal karyotype (Bulls A and B) and 2 that were heterozygous for the 7/21 translocation (B...
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| Veröffentlicht in: | Theriogenology 1996-10, Vol.46 (5), p.893-897 |
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| creator | Geshi, M. (Tohoku National Agricultural Experiment Station, Morioke, Iwate, Japan.) Sakaguchi, M Yonai, M Nagai, T Suzuki, O Hanada, H |
| description | A study was conducted to investigate the effect of the 7/21 Robertsonian translocation on fertilization and subsequent development of bovine oocytes matured in vitro. Semen from Japanese Black bulls, 2 with a normal karyotype (Bulls A and B) and 2 that were heterozygous for the 7/21 translocation (Bulls C and D), was used in this study. In vitro matured bovine oocytes were inseminated with frozen-thawed sperm capacitated with heparin. After insemination, oocytes were cultured at 38.5 degrees C on a monolayer of cumulus cells in TCM-199 supplemented with 5% superovulated cow serum and 0.5 mM sodium pyruvate in an atmosphere of 2% CO2 in air. Cleavage rate was evaluated at 54 h after insemination, and development of embryos to the blastocyst stage was observed 7 to 10 d post insemination. There was no difference in the fertilization rate among the 4 bulls. Although the cleavage rate of oocytes inseminated with semen from Bull C (heterozygote) was lower (P0.05) than that obtained with semen from Bull B (normal), the blastocyst formation rate did not differ among the 4 bulls. These results indicate that the 7/21 Robertsonian translocation had no effect on the fertilization and blastocyst formation rates of bovine in vitro matured oocytes |
| doi_str_mv | 10.1016/s0093-691x(96)00247-6 |
| format | Article |
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(Tohoku National Agricultural Experiment Station, Morioke, Iwate, Japan.) ; Sakaguchi, M ; Yonai, M ; Nagai, T ; Suzuki, O ; Hanada, H</creator><creatorcontrib>Geshi, M. (Tohoku National Agricultural Experiment Station, Morioke, Iwate, Japan.) ; Sakaguchi, M ; Yonai, M ; Nagai, T ; Suzuki, O ; Hanada, H</creatorcontrib><description>A study was conducted to investigate the effect of the 7/21 Robertsonian translocation on fertilization and subsequent development of bovine oocytes matured in vitro. Semen from Japanese Black bulls, 2 with a normal karyotype (Bulls A and B) and 2 that were heterozygous for the 7/21 translocation (Bulls C and D), was used in this study. In vitro matured bovine oocytes were inseminated with frozen-thawed sperm capacitated with heparin. After insemination, oocytes were cultured at 38.5 degrees C on a monolayer of cumulus cells in TCM-199 supplemented with 5% superovulated cow serum and 0.5 mM sodium pyruvate in an atmosphere of 2% CO2 in air. Cleavage rate was evaluated at 54 h after insemination, and development of embryos to the blastocyst stage was observed 7 to 10 d post insemination. There was no difference in the fertilization rate among the 4 bulls. Although the cleavage rate of oocytes inseminated with semen from Bull C (heterozygote) was lower (P0.05) than that obtained with semen from Bull B (normal), the blastocyst formation rate did not differ among the 4 bulls. These results indicate that the 7/21 Robertsonian translocation had no effect on the fertilization and blastocyst formation rates of bovine in vitro matured oocytes</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/s0093-691x(96)00247-6</identifier><identifier>PMID: 16727953</identifier><language>eng</language><publisher>United States</publisher><subject>BOVIN DE BOUCHERIE ; DESARROLLO EMBRIONARIO ; DEVELOPPEMENT EMBRYONNAIRE ; DIVISION CELLULAIRE ; DIVISION CELULAR ; EXPERIMENTACION IN VITRO ; EXPERIMENTATION IN VITRO ; FECONDATION ; FECUNDACION ; FERTILIDAD ; FERTILITE ; GANADO DE CARNE ; HETEROCIGOTOS ; HETEROZYGOTE ; MACHO ; MADURACION ; MALE ; MATURATION ; MORTALIDAD ; MORTALITE ; OVULE ; OVULO ; RACE (ANIMAL) ; RAZAS (ANIMALES) ; TAUREAU ; TORO ; TRANSLOCACION DE CROMOSOMAS ; TRANSLOCATION CHROMOSOMIQUE</subject><ispartof>Theriogenology, 1996-10, Vol.46 (5), p.893-897</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16727953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geshi, M. (Tohoku National Agricultural Experiment Station, Morioke, Iwate, Japan.)</creatorcontrib><creatorcontrib>Sakaguchi, M</creatorcontrib><creatorcontrib>Yonai, M</creatorcontrib><creatorcontrib>Nagai, T</creatorcontrib><creatorcontrib>Suzuki, O</creatorcontrib><creatorcontrib>Hanada, H</creatorcontrib><title>Effects of the 7/21 Robertsonian translocation on fertilization rates and preimplantation development of bovine oocytes in vitro</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>A study was conducted to investigate the effect of the 7/21 Robertsonian translocation on fertilization and subsequent development of bovine oocytes matured in vitro. Semen from Japanese Black bulls, 2 with a normal karyotype (Bulls A and B) and 2 that were heterozygous for the 7/21 translocation (Bulls C and D), was used in this study. In vitro matured bovine oocytes were inseminated with frozen-thawed sperm capacitated with heparin. After insemination, oocytes were cultured at 38.5 degrees C on a monolayer of cumulus cells in TCM-199 supplemented with 5% superovulated cow serum and 0.5 mM sodium pyruvate in an atmosphere of 2% CO2 in air. Cleavage rate was evaluated at 54 h after insemination, and development of embryos to the blastocyst stage was observed 7 to 10 d post insemination. There was no difference in the fertilization rate among the 4 bulls. Although the cleavage rate of oocytes inseminated with semen from Bull C (heterozygote) was lower (P0.05) than that obtained with semen from Bull B (normal), the blastocyst formation rate did not differ among the 4 bulls. These results indicate that the 7/21 Robertsonian translocation had no effect on the fertilization and blastocyst formation rates of bovine in vitro matured oocytes</description><subject>BOVIN DE BOUCHERIE</subject><subject>DESARROLLO EMBRIONARIO</subject><subject>DEVELOPPEMENT EMBRYONNAIRE</subject><subject>DIVISION CELLULAIRE</subject><subject>DIVISION CELULAR</subject><subject>EXPERIMENTACION IN VITRO</subject><subject>EXPERIMENTATION IN VITRO</subject><subject>FECONDATION</subject><subject>FECUNDACION</subject><subject>FERTILIDAD</subject><subject>FERTILITE</subject><subject>GANADO DE CARNE</subject><subject>HETEROCIGOTOS</subject><subject>HETEROZYGOTE</subject><subject>MACHO</subject><subject>MADURACION</subject><subject>MALE</subject><subject>MATURATION</subject><subject>MORTALIDAD</subject><subject>MORTALITE</subject><subject>OVULE</subject><subject>OVULO</subject><subject>RACE (ANIMAL)</subject><subject>RAZAS (ANIMALES)</subject><subject>TAUREAU</subject><subject>TORO</subject><subject>TRANSLOCACION DE CROMOSOMAS</subject><subject>TRANSLOCATION CHROMOSOMIQUE</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNo1kNtKAzEQhoMotlZfQFByJXqxmsM22VxKqQcQBA_g3ZLNQSO7yZqkxXrlo7ulFYYZmPn-n5kB4ASjS4wwu0oICVowgb_PBbtAiJS8YDtgjCsuCkoo3gXjf-RtBA5S-kQIUcbwPhhhxgkXUzoGv3NrjcoJBgvzh4H8imD4FBoTcwreSQ9zlD61QcnsgodD2GHmWvezaUSZTYLSa9hH47q-lT5vJtosTRv6zvi8dm_C0nkDQ1CrtcJ5uHQ5hkOwZ2WbzNG2TsDrzfxldlc8PN7ez64fCksJzkXVaM4ksRQp1VBqGSFTppQxRNmSk7JSWiGuBbdSV0ThEgnNKjscjKcETTWdgLONbx_D18KkXHcuKdMO-5qwSDWvOBNDGsDTLbhoOqPrPrpOxlX9_7MBON4AVoZavkeX6tdnwWhZDeo_kGB6ig</recordid><startdate>19961001</startdate><enddate>19961001</enddate><creator>Geshi, M. 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(Tohoku National Agricultural Experiment Station, Morioke, Iwate, Japan.) ; Sakaguchi, M ; Yonai, M ; Nagai, T ; Suzuki, O ; Hanada, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f321t-8bd76a2f30ccb33f62256ccee2cf47248cdc07d97fad82c1409d68f03615205d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>BOVIN DE BOUCHERIE</topic><topic>DESARROLLO EMBRIONARIO</topic><topic>DEVELOPPEMENT EMBRYONNAIRE</topic><topic>DIVISION CELLULAIRE</topic><topic>DIVISION CELULAR</topic><topic>EXPERIMENTACION IN VITRO</topic><topic>EXPERIMENTATION IN VITRO</topic><topic>FECONDATION</topic><topic>FECUNDACION</topic><topic>FERTILIDAD</topic><topic>FERTILITE</topic><topic>GANADO DE CARNE</topic><topic>HETEROCIGOTOS</topic><topic>HETEROZYGOTE</topic><topic>MACHO</topic><topic>MADURACION</topic><topic>MALE</topic><topic>MATURATION</topic><topic>MORTALIDAD</topic><topic>MORTALITE</topic><topic>OVULE</topic><topic>OVULO</topic><topic>RACE (ANIMAL)</topic><topic>RAZAS (ANIMALES)</topic><topic>TAUREAU</topic><topic>TORO</topic><topic>TRANSLOCACION DE CROMOSOMAS</topic><topic>TRANSLOCATION CHROMOSOMIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Geshi, M. (Tohoku National Agricultural Experiment Station, Morioke, Iwate, Japan.)</creatorcontrib><creatorcontrib>Sakaguchi, M</creatorcontrib><creatorcontrib>Yonai, M</creatorcontrib><creatorcontrib>Nagai, T</creatorcontrib><creatorcontrib>Suzuki, O</creatorcontrib><creatorcontrib>Hanada, H</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geshi, M. (Tohoku National Agricultural Experiment Station, Morioke, Iwate, Japan.)</au><au>Sakaguchi, M</au><au>Yonai, M</au><au>Nagai, T</au><au>Suzuki, O</au><au>Hanada, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the 7/21 Robertsonian translocation on fertilization rates and preimplantation development of bovine oocytes in vitro</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>1996-10-01</date><risdate>1996</risdate><volume>46</volume><issue>5</issue><spage>893</spage><epage>897</epage><pages>893-897</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>A study was conducted to investigate the effect of the 7/21 Robertsonian translocation on fertilization and subsequent development of bovine oocytes matured in vitro. Semen from Japanese Black bulls, 2 with a normal karyotype (Bulls A and B) and 2 that were heterozygous for the 7/21 translocation (Bulls C and D), was used in this study. In vitro matured bovine oocytes were inseminated with frozen-thawed sperm capacitated with heparin. After insemination, oocytes were cultured at 38.5 degrees C on a monolayer of cumulus cells in TCM-199 supplemented with 5% superovulated cow serum and 0.5 mM sodium pyruvate in an atmosphere of 2% CO2 in air. Cleavage rate was evaluated at 54 h after insemination, and development of embryos to the blastocyst stage was observed 7 to 10 d post insemination. There was no difference in the fertilization rate among the 4 bulls. Although the cleavage rate of oocytes inseminated with semen from Bull C (heterozygote) was lower (P0.05) than that obtained with semen from Bull B (normal), the blastocyst formation rate did not differ among the 4 bulls. These results indicate that the 7/21 Robertsonian translocation had no effect on the fertilization and blastocyst formation rates of bovine in vitro matured oocytes</abstract><cop>United States</cop><pmid>16727953</pmid><doi>10.1016/s0093-691x(96)00247-6</doi><tpages>5</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | BOVIN DE BOUCHERIE DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE DIVISION CELLULAIRE DIVISION CELULAR EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO FECONDATION FECUNDACION FERTILIDAD FERTILITE GANADO DE CARNE HETEROCIGOTOS HETEROZYGOTE MACHO MADURACION MALE MATURATION MORTALIDAD MORTALITE OVULE OVULO RACE (ANIMAL) RAZAS (ANIMALES) TAUREAU TORO TRANSLOCACION DE CROMOSOMAS TRANSLOCATION CHROMOSOMIQUE |
| title | Effects of the 7/21 Robertsonian translocation on fertilization rates and preimplantation development of bovine oocytes in vitro |
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