The ability of the restriction endonuclease EcoRI to digest hemi-methylated versus fully cytosine-methylated DNA of the herpes tk promoter region
Regulation of gene transcription appears to be influenced by cytosine methylation in vertebrates. Gene systems that are particularly sensitive to DNA methylation could be helpful in elucidating the inactivation mechanism, and helpful in determining which CpG's or group of CpG's are involve...
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| Veröffentlicht in: | Gene 1988-12, Vol.74 (1), p.147-149 |
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| container_title | Gene |
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| creator | Tasseron-de Jong, Judith G. Aker, José Giphart-Gassler, Micheline |
| description | Regulation of gene transcription appears to be influenced by cytosine methylation in vertebrates. Gene systems that are particularly sensitive to DNA methylation could be helpful in elucidating the inactivation mechanism, and helpful in determining which CpG's or group of CpG's are involved in the actual inactivation of transcription. We have such a gene system, in which a transfected herpesvirus tk gene is functionally inactivated by a low amount of DNA methylation. (DBO) |
| doi_str_mv | 10.1016/0378-1119(88)90272-7 |
| format | Article |
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Genome</subject><subject>herpes simplex virus</subject><subject>human cells</subject><subject>Humans</subject><subject>Methylation</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Simplexvirus - genetics</subject><subject>Thymidine Kinase - genetics</subject><subject>transfected genes</subject><subject>Viral Proteins - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKkPhDUDyAlWwCPgnsZ0NUtUfqFSBhMracuybjiGJB9uplMfgjethhhGr1l5YV-e799g-CL2m5AMlVHwkXKqKUtq-U-p9S5hklXyCVlTJtiKEq6dodUCeoxcp_SRlNQ07QkdMNbUifIX-3KwBm84PPi849DiXMkLK0dvsw4RhcmGa7QAmAb6w4fsVzgE7f1sYvIbRVyPk9TKYDA7fQUxzwv08DAu2Sw7JT_A_cP719J_JGuIGEs6_8CaGMWSIxfe2WL5Ez3ozJHi1P4_Rj8uLm7Mv1fW3z1dnp9eVranMVdPxrpatYE3Nne2ogLZsbnjvGGeUFYEb0TbKqd6AEa5TrGdWAO2l7TrBj9HJbm7x_z2X5-jRJwvDYCYIc9JSSVHXsn4UpIIqxiUvYL0DbQwpRej1JvrRxEVToreR6W0eepuHVkr_jUzL0vZmP3_uRnCHpn1GRX-7102yZuijmaxPB0yIRirGCvZph0H5tDsPUSfrYbLgfASbtQv-4XvcA5yhtOk</recordid><startdate>19881225</startdate><enddate>19881225</enddate><creator>Tasseron-de Jong, Judith G.</creator><creator>Aker, José</creator><creator>Giphart-Gassler, Micheline</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19881225</creationdate><title>The ability of the restriction endonuclease EcoRI to digest hemi-methylated versus fully cytosine-methylated DNA of the herpes tk promoter region</title><author>Tasseron-de Jong, Judith G. ; Aker, José ; Giphart-Gassler, Micheline</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-5b3b47962543dcb16e9e9e3a3fd232126253a6958d8faea6db82f2c6e1f7cbb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>5-Azacytidine</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Deoxyribonuclease EcoRI - metabolism</topic><topic>DNA, Viral - metabolism</topic><topic>Enzyme Induction</topic><topic>Fundamental and applied biological sciences. 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Gene systems that are particularly sensitive to DNA methylation could be helpful in elucidating the inactivation mechanism, and helpful in determining which CpG's or group of CpG's are involved in the actual inactivation of transcription. We have such a gene system, in which a transfected herpesvirus tk gene is functionally inactivated by a low amount of DNA methylation. (DBO)</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2854803</pmid><doi>10.1016/0378-1119(88)90272-7</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1988-12, Vol.74 (1), p.147-149 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78764474 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 5-Azacytidine Biological and medical sciences Cell Line Deoxyribonuclease EcoRI - metabolism DNA, Viral - metabolism Enzyme Induction Fundamental and applied biological sciences. Psychology gene activity Genes, Viral Genes. Genome herpes simplex virus human cells Humans Methylation Molecular and cellular biology Molecular genetics Promoter Regions, Genetic Simplexvirus - genetics Thymidine Kinase - genetics transfected genes Viral Proteins - genetics |
| title | The ability of the restriction endonuclease EcoRI to digest hemi-methylated versus fully cytosine-methylated DNA of the herpes tk promoter region |
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