Semisynthetic Preparation of 1-O-hexadecyl-2-acetyl-sn-glyceryl-3- phosphorylcholine (Platelet Activating Factor)
1-O-hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor, PAF), (1.9 μmol) was prepared from the total lipid extract of the protozoan Tetrahymena pyriformis 9 × 10 cells. The procedure involved mild alkaline hydrolysis of the total lipids, followed by acetylation and purifi...
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| Veröffentlicht in: | Zeitschrift für Naturforschung C. A journal of biosciences 1988-10, Vol.43 (9), p.665-670 |
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| container_title | Zeitschrift für Naturforschung C. A journal of biosciences |
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| creator | Lekka, Marilena E. Tsoukatos, Demokritos C. Tselepis, Alexandras D. Kapoulas, Vassilios M. |
| description | 1-O-hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor, PAF), (1.9 μmol) was prepared from the total lipid extract of the protozoan Tetrahymena pyriformis 9 × 10
cells. The procedure involved mild alkaline hydrolysis of the total lipids, followed by acetylation and purification of the product by preparative TLC and HPLC . The yield was 60 % with respect to the content of 1-O-alkyl-2-acyl-sn-glyceryl-3-phosphorylcholine in the total lipids, determined after preparative TLC . The alkyl side chain of the semisynthetic PAF was composed of hexadecyl residue. Our product was identified as PAF according to its biological activity, the chromatographic behaviour on TLC and HPLC , the physicochemical properties and the behaviour under treatment with PLA
and Lipase from Rhizopus arrhizus. The above procedure is proposed as a facile, inexpensive and convenient method. |
| doi_str_mv | 10.1515/znc-1988-9-1007 |
| format | Article |
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cells. The procedure involved mild alkaline hydrolysis of the total lipids, followed by acetylation and purification of the product by preparative TLC and HPLC . The yield was 60 % with respect to the content of 1-O-alkyl-2-acyl-sn-glyceryl-3-phosphorylcholine in the total lipids, determined after preparative TLC . The alkyl side chain of the semisynthetic PAF was composed of hexadecyl residue. Our product was identified as PAF according to its biological activity, the chromatographic behaviour on TLC and HPLC , the physicochemical properties and the behaviour under treatment with PLA
and Lipase from Rhizopus arrhizus. The above procedure is proposed as a facile, inexpensive and convenient method.</description><identifier>ISSN: 0939-5075</identifier><identifier>EISSN: 1865-7125</identifier><identifier>DOI: 10.1515/znc-1988-9-1007</identifier><identifier>PMID: 3245264</identifier><language>eng</language><publisher>Germany: Verlag der Zeitschrift für Naturforschung</publisher><subject>Animals ; Blood Platelets - drug effects ; Blood Platelets - physiology ; Chromatography, Gas ; Chromatography, High Pressure Liquid - methods ; Ether Lipids ; Lipids - isolation & purification ; Phospholipids ; Platelet Activating Factor ; Platelet Activating Factor - chemical synthesis ; Platelet Activating Factor - isolation & purification ; Platelet Activating Factor - pharmacology ; Platelets ; Rabbits ; Tetrahymena - metabolism ; Tetrahymena pyriformis</subject><ispartof>Zeitschrift für Naturforschung C. A journal of biosciences, 1988-10, Vol.43 (9), p.665-670</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-8178b84e20021e7e86ba0ff1b2594829088f2cb857ebeb1342ac9e3e41b440963</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3245264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lekka, Marilena E.</creatorcontrib><creatorcontrib>Tsoukatos, Demokritos C.</creatorcontrib><creatorcontrib>Tselepis, Alexandras D.</creatorcontrib><creatorcontrib>Kapoulas, Vassilios M.</creatorcontrib><title>Semisynthetic Preparation of 1-O-hexadecyl-2-acetyl-sn-glyceryl-3- phosphorylcholine (Platelet Activating Factor)</title><title>Zeitschrift für Naturforschung C. A journal of biosciences</title><addtitle>Z Naturforsch C</addtitle><description>1-O-hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor, PAF), (1.9 μmol) was prepared from the total lipid extract of the protozoan Tetrahymena pyriformis 9 × 10
cells. The procedure involved mild alkaline hydrolysis of the total lipids, followed by acetylation and purification of the product by preparative TLC and HPLC . The yield was 60 % with respect to the content of 1-O-alkyl-2-acyl-sn-glyceryl-3-phosphorylcholine in the total lipids, determined after preparative TLC . The alkyl side chain of the semisynthetic PAF was composed of hexadecyl residue. Our product was identified as PAF according to its biological activity, the chromatographic behaviour on TLC and HPLC , the physicochemical properties and the behaviour under treatment with PLA
and Lipase from Rhizopus arrhizus. The above procedure is proposed as a facile, inexpensive and convenient method.</description><subject>Animals</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - physiology</subject><subject>Chromatography, Gas</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Ether Lipids</subject><subject>Lipids - isolation & purification</subject><subject>Phospholipids</subject><subject>Platelet Activating Factor</subject><subject>Platelet Activating Factor - chemical synthesis</subject><subject>Platelet Activating Factor - isolation & purification</subject><subject>Platelet Activating Factor - pharmacology</subject><subject>Platelets</subject><subject>Rabbits</subject><subject>Tetrahymena - metabolism</subject><subject>Tetrahymena pyriformis</subject><issn>0939-5075</issn><issn>1865-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1rGzEQxUVpSZ20554KeyrNQY0-V9KtJjRJIZBA27PQyrP2BnnlSNok278-Mja99TDMG-bNg_kh9ImSb1RSefF39JgarbHBlBD1Bi2obiVWlMm3aEEMN1gSJd-j05wfCOGtVPIEnXAmJGvFAj3-gu2Q57FsoAy-uU-wc8mVIY5N7BuK7_AGXtwK_Bwww85DqSKPeB1mD6lqjpvdJuZadfKbGIYRmq_3wRUIUJqlL8NTzRvXzZXzJabzD-hd70KGj8d-hv5c_fh9eYNv765_Xi5vseeGFKyp0p0WwAhhFBTotnOk72nHpBGaGaJ1z3ynpYIOOsoFc94AB0E7IYhp-Rn6csjdpfg4QS62PuohBDdCnLJVWkmtDanGi4PRp5hzgt7u0rB1abaU2D1kWyHbPWRr7B5yvfh8jJ66Laz--Y9U6_77Yf_sQoG0gnWa5irsQ5zSWJ_-X7Lg-962kr8CPAyNxA</recordid><startdate>19881001</startdate><enddate>19881001</enddate><creator>Lekka, Marilena E.</creator><creator>Tsoukatos, Demokritos C.</creator><creator>Tselepis, Alexandras D.</creator><creator>Kapoulas, Vassilios M.</creator><general>Verlag der Zeitschrift für Naturforschung</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19881001</creationdate><title>Semisynthetic Preparation of 1-O-hexadecyl-2-acetyl-sn-glyceryl-3- phosphorylcholine (Platelet Activating Factor)</title><author>Lekka, Marilena E. ; Tsoukatos, Demokritos C. ; Tselepis, Alexandras D. ; Kapoulas, Vassilios M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-8178b84e20021e7e86ba0ff1b2594829088f2cb857ebeb1342ac9e3e41b440963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - physiology</topic><topic>Chromatography, Gas</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Ether Lipids</topic><topic>Lipids - isolation & purification</topic><topic>Phospholipids</topic><topic>Platelet Activating Factor</topic><topic>Platelet Activating Factor - chemical synthesis</topic><topic>Platelet Activating Factor - isolation & purification</topic><topic>Platelet Activating Factor - pharmacology</topic><topic>Platelets</topic><topic>Rabbits</topic><topic>Tetrahymena - metabolism</topic><topic>Tetrahymena pyriformis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lekka, Marilena E.</creatorcontrib><creatorcontrib>Tsoukatos, Demokritos C.</creatorcontrib><creatorcontrib>Tselepis, Alexandras D.</creatorcontrib><creatorcontrib>Kapoulas, Vassilios M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Zeitschrift für Naturforschung C. A journal of biosciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lekka, Marilena E.</au><au>Tsoukatos, Demokritos C.</au><au>Tselepis, Alexandras D.</au><au>Kapoulas, Vassilios M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Semisynthetic Preparation of 1-O-hexadecyl-2-acetyl-sn-glyceryl-3- phosphorylcholine (Platelet Activating Factor)</atitle><jtitle>Zeitschrift für Naturforschung C. A journal of biosciences</jtitle><addtitle>Z Naturforsch C</addtitle><date>1988-10-01</date><risdate>1988</risdate><volume>43</volume><issue>9</issue><spage>665</spage><epage>670</epage><pages>665-670</pages><issn>0939-5075</issn><eissn>1865-7125</eissn><abstract>1-O-hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor, PAF), (1.9 μmol) was prepared from the total lipid extract of the protozoan Tetrahymena pyriformis 9 × 10
cells. The procedure involved mild alkaline hydrolysis of the total lipids, followed by acetylation and purification of the product by preparative TLC and HPLC . The yield was 60 % with respect to the content of 1-O-alkyl-2-acyl-sn-glyceryl-3-phosphorylcholine in the total lipids, determined after preparative TLC . The alkyl side chain of the semisynthetic PAF was composed of hexadecyl residue. Our product was identified as PAF according to its biological activity, the chromatographic behaviour on TLC and HPLC , the physicochemical properties and the behaviour under treatment with PLA
and Lipase from Rhizopus arrhizus. The above procedure is proposed as a facile, inexpensive and convenient method.</abstract><cop>Germany</cop><pub>Verlag der Zeitschrift für Naturforschung</pub><pmid>3245264</pmid><doi>10.1515/znc-1988-9-1007</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0939-5075 |
| ispartof | Zeitschrift für Naturforschung C. A journal of biosciences, 1988-10, Vol.43 (9), p.665-670 |
| issn | 0939-5075 1865-7125 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Blood Platelets - drug effects Blood Platelets - physiology Chromatography, Gas Chromatography, High Pressure Liquid - methods Ether Lipids Lipids - isolation & purification Phospholipids Platelet Activating Factor Platelet Activating Factor - chemical synthesis Platelet Activating Factor - isolation & purification Platelet Activating Factor - pharmacology Platelets Rabbits Tetrahymena - metabolism Tetrahymena pyriformis |
| title | Semisynthetic Preparation of 1-O-hexadecyl-2-acetyl-sn-glyceryl-3- phosphorylcholine (Platelet Activating Factor) |
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