Immunochemical studies of human placental alkaline phosphatase in normal and neoplastic tissues
Human placental alkaline phosphatase (hPLAP) is normally present in plasma membranes of syncytiotrophoblast microvilli. hPLAP is expressed by many malignant tumors and is considered to be useful as a tumor marker. The objective of the work was to develop immunochemical methods for detection of this...
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| Veröffentlicht in: | Advances in enzyme regulation 1988, Vol.27, p.345-352 |
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| container_title | Advances in enzyme regulation |
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| creator | Vengerov, Yu.Yu Gudima, S.O. Voronov, A.V. Votrin, I.I. |
| description | Human placental alkaline phosphatase (hPLAP) is normally present in plasma membranes of syncytiotrophoblast microvilli. hPLAP is expressed by many malignant tumors and is considered to be useful as a tumor marker. The objective of the work was to develop immunochemical methods for detection of this marker. hPLAP was isolated from placenta using a procedure with preparative isoelectric focusing as a final purification step, and was used to obtain rabbit antisera. Anti-hPLAP antibodies were obtained by affinity chromatography on the immobilized hPLAP antigen and were used in immunoenzyme tests for detection of hPLAP in sera and tissues of cancer patients with different malignancies. Anti-hPLAP antibodies were also used for localization of hPLAP on the histological level. The described methods may be of use in cancer diagnostics and therapy. |
| doi_str_mv | 10.1016/0065-2571(88)90025-8 |
| format | Article |
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The objective of the work was to develop immunochemical methods for detection of this marker. hPLAP was isolated from placenta using a procedure with preparative isoelectric focusing as a final purification step, and was used to obtain rabbit antisera. Anti-hPLAP antibodies were obtained by affinity chromatography on the immobilized hPLAP antigen and were used in immunoenzyme tests for detection of hPLAP in sera and tissues of cancer patients with different malignancies. Anti-hPLAP antibodies were also used for localization of hPLAP on the histological level. 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The described methods may be of use in cancer diagnostics and therapy.</description><subject>Alkaline Phosphatase</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Cystadenocarcinoma - enzymology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>GPI-Linked Proteins</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Isoenzymes - analysis</subject><subject>Isoenzymes - blood</subject><subject>Ovarian Neoplasms - enzymology</subject><subject>Placenta - enzymology</subject><subject>Uterine Neoplasms - enzymology</subject><issn>0065-2571</issn><issn>1873-2437</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMo4zj6BgpZiS6quUyadCPI4GVgwI2uQyY9pdE2rUkr-PamzuDS1Vl8_7l9CJ1TckMJzW8JyUXGhKRXSl0XhDCRqQM0p0ryjC25PETzv8gxOonxnRDKZC5naMaJXOaczpFet-3oO1tD66xpcBzG0kHEXYXrsTUe942x4IeETPNhGucB93UX-9oMJgJ2HvsutBP2JfbQpXwcnMWDi3GEeIqOKtNEONvXBXp7fHhdPWebl6f16n6TWS7kkBnIhcmBGZvOLwgvDGdgiNmWipAtEKqooLIAnpDl1Copl4wplQsrKmY5X6DL3dw-dJ9p76BbFy00jUk3jVFLJYUiqkjB5S5oQxdjgEr3wbUmfGtK9ORVT9L0JE0rpX-9apXaLvbzx20L5V_TXmTidzsO6ckvB0FH68BbKF0AO-iyc_8v-AH0VYfI</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Vengerov, Yu.Yu</creator><creator>Gudima, S.O.</creator><creator>Voronov, A.V.</creator><creator>Votrin, I.I.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1988</creationdate><title>Immunochemical studies of human placental alkaline phosphatase in normal and neoplastic tissues</title><author>Vengerov, Yu.Yu ; Gudima, S.O. ; Voronov, A.V. ; Votrin, I.I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-ae65a6e2ac1879039a32ea0abd800be01815179e3039c31c8774228865c5f2c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Alkaline Phosphatase</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Cystadenocarcinoma - enzymology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>GPI-Linked Proteins</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Isoenzymes - analysis</topic><topic>Isoenzymes - blood</topic><topic>Ovarian Neoplasms - enzymology</topic><topic>Placenta - enzymology</topic><topic>Uterine Neoplasms - enzymology</topic><toplevel>online_resources</toplevel><creatorcontrib>Vengerov, Yu.Yu</creatorcontrib><creatorcontrib>Gudima, S.O.</creatorcontrib><creatorcontrib>Voronov, A.V.</creatorcontrib><creatorcontrib>Votrin, I.I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Advances in enzyme regulation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vengerov, Yu.Yu</au><au>Gudima, S.O.</au><au>Voronov, A.V.</au><au>Votrin, I.I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunochemical studies of human placental alkaline phosphatase in normal and neoplastic tissues</atitle><jtitle>Advances in enzyme regulation</jtitle><addtitle>Adv Enzyme Regul</addtitle><date>1988</date><risdate>1988</risdate><volume>27</volume><spage>345</spage><epage>352</epage><pages>345-352</pages><issn>0065-2571</issn><eissn>1873-2437</eissn><abstract>Human placental alkaline phosphatase (hPLAP) is normally present in plasma membranes of syncytiotrophoblast microvilli. hPLAP is expressed by many malignant tumors and is considered to be useful as a tumor marker. 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| identifier | ISSN: 0065-2571 |
| ispartof | Advances in enzyme regulation, 1988, Vol.27, p.345-352 |
| issn | 0065-2571 1873-2437 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alkaline Phosphatase Biomarkers, Tumor - analysis Cystadenocarcinoma - enzymology Enzyme-Linked Immunosorbent Assay Female GPI-Linked Proteins Humans Immunoenzyme Techniques Isoenzymes - analysis Isoenzymes - blood Ovarian Neoplasms - enzymology Placenta - enzymology Uterine Neoplasms - enzymology |
| title | Immunochemical studies of human placental alkaline phosphatase in normal and neoplastic tissues |
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