Characterization of a novel nuclear envelope protein restricted to certain cell types
The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelo...
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| Veröffentlicht in: | Journal of cell science 1988-06, Vol.90 (2), p.237-245 |
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| container_title | Journal of cell science |
| container_volume | 90 |
| creator | LORD, J. M THICK, J. A BUNCE, C. M TAYLOR, A. M. R GALLIMORE, P. H MILLS, D BROWN, G |
| description | The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that have a similar molecular mass. Antigen extraction and immunoelectron microscope studies revealed that the AGF2.3 protein is an integral membrane protein present at both the inner and outer aspects of the nuclear envelope. The protein is not associated with nuclear pores and therefore is distinct from pore complex proteins. The AGF2.3 protein does not have ATPase activity. Therefore, this protein is also distinct from a myosin heavy chain-like ATPase that is associated with the nuclear envelope. The AGF2.3 antibody identifies a novel nuclear envelope protein. Further studies of the biochemical nature of the AGF2.3 protein should provide insight into novel cellular processes at the nuclear envelope relating to the lineage or maturation status of cells. |
| doi_str_mv | 10.1242/jcs.90.2.237 |
| format | Article |
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M ; THICK, J. A ; BUNCE, C. M ; TAYLOR, A. M. R ; GALLIMORE, P. H ; MILLS, D ; BROWN, G</creator><creatorcontrib>LORD, J. M ; THICK, J. A ; BUNCE, C. M ; TAYLOR, A. M. R ; GALLIMORE, P. H ; MILLS, D ; BROWN, G</creatorcontrib><description>The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that have a similar molecular mass. Antigen extraction and immunoelectron microscope studies revealed that the AGF2.3 protein is an integral membrane protein present at both the inner and outer aspects of the nuclear envelope. The protein is not associated with nuclear pores and therefore is distinct from pore complex proteins. The AGF2.3 protein does not have ATPase activity. Therefore, this protein is also distinct from a myosin heavy chain-like ATPase that is associated with the nuclear envelope. The AGF2.3 antibody identifies a novel nuclear envelope protein. Further studies of the biochemical nature of the AGF2.3 protein should provide insight into novel cellular processes at the nuclear envelope relating to the lineage or maturation status of cells.</description><identifier>ISSN: 0021-9533</identifier><identifier>EISSN: 1477-9137</identifier><identifier>DOI: 10.1242/jcs.90.2.237</identifier><identifier>PMID: 3246520</identifier><identifier>CODEN: JNCSAI</identifier><language>eng</language><publisher>Cambridge: Company of Biologists</publisher><subject>Antibodies, Monoclonal ; Biological and medical sciences ; Cell Differentiation ; Cell Line ; Cell nucleus ; Cell structures and functions ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunohistochemistry ; Microscopy, Electron ; Molecular and cellular biology ; Nuclear Envelope - analysis ; Nuclear Proteins - analysis</subject><ispartof>Journal of cell science, 1988-06, Vol.90 (2), p.237-245</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-290996226dccd7c8f91a5ee7ccaaf4629770610d3ddc2df42df4a32d463d3d93</citedby><cites>FETCH-LOGICAL-c349t-290996226dccd7c8f91a5ee7ccaaf4629770610d3ddc2df42df4a32d463d3d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7023937$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3246520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LORD, J. M</creatorcontrib><creatorcontrib>THICK, J. A</creatorcontrib><creatorcontrib>BUNCE, C. M</creatorcontrib><creatorcontrib>TAYLOR, A. M. R</creatorcontrib><creatorcontrib>GALLIMORE, P. H</creatorcontrib><creatorcontrib>MILLS, D</creatorcontrib><creatorcontrib>BROWN, G</creatorcontrib><title>Characterization of a novel nuclear envelope protein restricted to certain cell types</title><title>Journal of cell science</title><addtitle>J Cell Sci</addtitle><description>The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that have a similar molecular mass. Antigen extraction and immunoelectron microscope studies revealed that the AGF2.3 protein is an integral membrane protein present at both the inner and outer aspects of the nuclear envelope. The protein is not associated with nuclear pores and therefore is distinct from pore complex proteins. The AGF2.3 protein does not have ATPase activity. Therefore, this protein is also distinct from a myosin heavy chain-like ATPase that is associated with the nuclear envelope. The AGF2.3 antibody identifies a novel nuclear envelope protein. Further studies of the biochemical nature of the AGF2.3 protein should provide insight into novel cellular processes at the nuclear envelope relating to the lineage or maturation status of cells.</description><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cell nucleus</subject><subject>Cell structures and functions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Microscopy, Electron</subject><subject>Molecular and cellular biology</subject><subject>Nuclear Envelope - analysis</subject><subject>Nuclear Proteins - analysis</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LAzEQxYMotVZvXoUcxJO75ms3zVGKX1DwUs9hTLK4ZbtZk6xQ_3pTungYhpn5zePxELqmpKRMsIetiaUiJSsZlydoToWUhaJcnqI5IYwWquL8HF3EuCWESKbkDM04E3XFyBx9rL4ggEkutL-QWt9j32DAvf9xHe5H0zkI2PV58oPDQ_DJtT0OLqbQ5i-Lk8fGhQR5a1zX4bQfXLxEZw100V1NfYE2z0-b1Wuxfn95Wz2uC8OFSgVTRKmasdoaY6VZNopC5Zw0BqARdbYqSU2J5dYaZhtxKODMiprnneILdHeUzb6-x-xJ79p4cAG982PUcikrzqjI4P0RNMHHGFyjh9DuIOw1JfoQos4hakU00znEjN9MuuPnztl_eEot32-nO0QDXROgN238xyRhXGWZP4nqe0w</recordid><startdate>19880601</startdate><enddate>19880601</enddate><creator>LORD, J. M</creator><creator>THICK, J. A</creator><creator>BUNCE, C. M</creator><creator>TAYLOR, A. M. R</creator><creator>GALLIMORE, P. H</creator><creator>MILLS, D</creator><creator>BROWN, G</creator><general>Company of Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880601</creationdate><title>Characterization of a novel nuclear envelope protein restricted to certain cell types</title><author>LORD, J. M ; THICK, J. A ; BUNCE, C. M ; TAYLOR, A. M. R ; GALLIMORE, P. H ; MILLS, D ; BROWN, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-290996226dccd7c8f91a5ee7ccaaf4629770610d3ddc2df42df4a32d463d3d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cell nucleus</topic><topic>Cell structures and functions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Microscopy, Electron</topic><topic>Molecular and cellular biology</topic><topic>Nuclear Envelope - analysis</topic><topic>Nuclear Proteins - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LORD, J. M</creatorcontrib><creatorcontrib>THICK, J. A</creatorcontrib><creatorcontrib>BUNCE, C. M</creatorcontrib><creatorcontrib>TAYLOR, A. M. R</creatorcontrib><creatorcontrib>GALLIMORE, P. H</creatorcontrib><creatorcontrib>MILLS, D</creatorcontrib><creatorcontrib>BROWN, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LORD, J. M</au><au>THICK, J. A</au><au>BUNCE, C. M</au><au>TAYLOR, A. M. R</au><au>GALLIMORE, P. H</au><au>MILLS, D</au><au>BROWN, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a novel nuclear envelope protein restricted to certain cell types</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>1988-06-01</date><risdate>1988</risdate><volume>90</volume><issue>2</issue><spage>237</spage><epage>245</epage><pages>237-245</pages><issn>0021-9533</issn><eissn>1477-9137</eissn><coden>JNCSAI</coden><abstract>The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that have a similar molecular mass. Antigen extraction and immunoelectron microscope studies revealed that the AGF2.3 protein is an integral membrane protein present at both the inner and outer aspects of the nuclear envelope. The protein is not associated with nuclear pores and therefore is distinct from pore complex proteins. The AGF2.3 protein does not have ATPase activity. Therefore, this protein is also distinct from a myosin heavy chain-like ATPase that is associated with the nuclear envelope. The AGF2.3 antibody identifies a novel nuclear envelope protein. Further studies of the biochemical nature of the AGF2.3 protein should provide insight into novel cellular processes at the nuclear envelope relating to the lineage or maturation status of cells.</abstract><cop>Cambridge</cop><pub>Company of Biologists</pub><pmid>3246520</pmid><doi>10.1242/jcs.90.2.237</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of cell science, 1988-06, Vol.90 (2), p.237-245 |
| issn | 0021-9533 1477-9137 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78753214 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Company of Biologists |
| subjects | Antibodies, Monoclonal Biological and medical sciences Cell Differentiation Cell Line Cell nucleus Cell structures and functions Fundamental and applied biological sciences. Psychology Humans Immunohistochemistry Microscopy, Electron Molecular and cellular biology Nuclear Envelope - analysis Nuclear Proteins - analysis |
| title | Characterization of a novel nuclear envelope protein restricted to certain cell types |
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