Reduction of the adherence of Streptococcus sobrinus insoluble α- d-glucan by endo-(1→3)-α- d-glucanase
Insoluble α- d-glucan, previously formed on a glass surface from sucrose by the action of cell-free d-glucosyltransferases of Streptococcus sobrinus OMZ176, was significantly removed by a purified preparation of endo-(1→3)-α- d-glucanase (mutanase) from a strain of Pseudomonas sp. Almost complete di...
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| Veröffentlicht in: | Carbohydrate research 1988-11, Vol.182 (2), p.277-286 |
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| creator | Inoue, Masakazu Yakushiji, Tsuyoshi Katsuki, Midori Kudo, Naoko Koga, Toshihiko |
| description | Insoluble α-
d-glucan, previously formed on a glass surface from sucrose by the action of cell-free
d-glucosyltransferases of
Streptococcus sobrinus OMZ176, was significantly removed by a purified preparation of endo-(1→3)-α-
d-glucanase (mutanase) from a strain of
Pseudomonas sp. Almost complete dissociation of adherent glucan occurred at the highest enzyme concentration (40 mU/mL) tested. Synthesis and
de novo adherence on glass of the glucan was markedly inhibited by the presence of mutanase, even at low concentrations (4 mU/mL or less). When compared to native glucan, the mutanase-modified glucan samples (a) contained lower proportion of
d-(1→3) linkages; (b) showed lower susceptibility to mutanase and higher susceptibility to (1→6)-α-
d-glucanase (dextranase); (c) contained larger amounts of low-molecular-weight fractions; (d) had lower intrinsic viscosities; (e) showed higher
S. sobrinus cell-agglutinating activities; and (f) consisted of looser entwinement of coalescent single-stranded fibrils (a major component) and shorter double-stranded fibrils (a minor one). |
| doi_str_mv | 10.1016/0008-6215(88)84008-4 |
| format | Article |
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d-glucan, previously formed on a glass surface from sucrose by the action of cell-free
d-glucosyltransferases of
Streptococcus sobrinus OMZ176, was significantly removed by a purified preparation of endo-(1→3)-α-
d-glucanase (mutanase) from a strain of
Pseudomonas sp. Almost complete dissociation of adherent glucan occurred at the highest enzyme concentration (40 mU/mL) tested. Synthesis and
de novo adherence on glass of the glucan was markedly inhibited by the presence of mutanase, even at low concentrations (4 mU/mL or less). When compared to native glucan, the mutanase-modified glucan samples (a) contained lower proportion of
d-(1→3) linkages; (b) showed lower susceptibility to mutanase and higher susceptibility to (1→6)-α-
d-glucanase (dextranase); (c) contained larger amounts of low-molecular-weight fractions; (d) had lower intrinsic viscosities; (e) showed higher
S. sobrinus cell-agglutinating activities; and (f) consisted of looser entwinement of coalescent single-stranded fibrils (a major component) and shorter double-stranded fibrils (a minor one).</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/0008-6215(88)84008-4</identifier><identifier>PMID: 2977294</identifier><identifier>CODEN: CRBRAT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adhesiveness ; Agglutination Tests ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Carbohydrates ; Fundamental and applied biological sciences. Psychology ; Glucans - metabolism ; Glucosyltransferases - isolation & purification ; Glycoside Hydrolases - metabolism ; Hydrolysis ; Methylation ; Microscopy, Electron ; Molecular Weight ; Other biological molecules ; Polysaccharides, Bacterial ; Solubility ; Streptococcus ; Viscosity</subject><ispartof>Carbohydrate research, 1988-11, Vol.182 (2), p.277-286</ispartof><rights>1988</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-40ca2942750447bd4d047674412134c6d6c9ee2dd64c09180c804dc32a1a86a53</citedby><cites>FETCH-LOGICAL-c386t-40ca2942750447bd4d047674412134c6d6c9ee2dd64c09180c804dc32a1a86a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0008621588840084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6703011$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2977294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, Masakazu</creatorcontrib><creatorcontrib>Yakushiji, Tsuyoshi</creatorcontrib><creatorcontrib>Katsuki, Midori</creatorcontrib><creatorcontrib>Kudo, Naoko</creatorcontrib><creatorcontrib>Koga, Toshihiko</creatorcontrib><title>Reduction of the adherence of Streptococcus sobrinus insoluble α- d-glucan by endo-(1→3)-α- d-glucanase</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>Insoluble α-
d-glucan, previously formed on a glass surface from sucrose by the action of cell-free
d-glucosyltransferases of
Streptococcus sobrinus OMZ176, was significantly removed by a purified preparation of endo-(1→3)-α-
d-glucanase (mutanase) from a strain of
Pseudomonas sp. Almost complete dissociation of adherent glucan occurred at the highest enzyme concentration (40 mU/mL) tested. Synthesis and
de novo adherence on glass of the glucan was markedly inhibited by the presence of mutanase, even at low concentrations (4 mU/mL or less). When compared to native glucan, the mutanase-modified glucan samples (a) contained lower proportion of
d-(1→3) linkages; (b) showed lower susceptibility to mutanase and higher susceptibility to (1→6)-α-
d-glucanase (dextranase); (c) contained larger amounts of low-molecular-weight fractions; (d) had lower intrinsic viscosities; (e) showed higher
S. sobrinus cell-agglutinating activities; and (f) consisted of looser entwinement of coalescent single-stranded fibrils (a major component) and shorter double-stranded fibrils (a minor one).</description><subject>Adhesiveness</subject><subject>Agglutination Tests</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucans - metabolism</subject><subject>Glucosyltransferases - isolation & purification</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>Hydrolysis</subject><subject>Methylation</subject><subject>Microscopy, Electron</subject><subject>Molecular Weight</subject><subject>Other biological molecules</subject><subject>Polysaccharides, Bacterial</subject><subject>Solubility</subject><subject>Streptococcus</subject><subject>Viscosity</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1KJDEQx4MoOut6A4V-ENGHaJLOJJkXQWT9AEHYD9i3kK7UaHZ7OmPSveAF9gDexIt4CE9itzMMPvmUVP6_qhQ_QnY5O-aMqxPGmKFK8PGhMUdGDpVcIyNudEmlUL_XyWiFbJEvOf_pS6a02iSbYqK1mMgR-fsdfQdtiE0Rp0V7j4Xz95iwARwefrQJ522ECNDlIscqhaa_hCbHuqtqLF6eaeHpXd2Ba4rqscDGR3rIX_8_lUf0Y-gyfiUbU1dn3Fme2-TXxbef51f05vby-vzshkJpVEslA9fvJvSYSakrLz2TWmkpueClBOUVTBCF90oCm3DDwDDpoRSOO6PcuNwmB4u58xQfOsytnYUMWNeuwdhlq3tDgmneg3IBQoo5J5zaeQozlx4tZ3ZwbAeBdhBojbHvjq3s2_aW87tqhn7VtJTa5_vL3GVw9TS5BkJeYUqzkvHh99MFhr2LfwGTzRAG8T4khNb6GD7f4w2rW5ht</recordid><startdate>19881101</startdate><enddate>19881101</enddate><creator>Inoue, Masakazu</creator><creator>Yakushiji, Tsuyoshi</creator><creator>Katsuki, Midori</creator><creator>Kudo, Naoko</creator><creator>Koga, Toshihiko</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19881101</creationdate><title>Reduction of the adherence of Streptococcus sobrinus insoluble α- d-glucan by endo-(1→3)-α- d-glucanase</title><author>Inoue, Masakazu ; Yakushiji, Tsuyoshi ; Katsuki, Midori ; Kudo, Naoko ; Koga, Toshihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-40ca2942750447bd4d047674412134c6d6c9ee2dd64c09180c804dc32a1a86a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adhesiveness</topic><topic>Agglutination Tests</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucans - metabolism</topic><topic>Glucosyltransferases - isolation & purification</topic><topic>Glycoside Hydrolases - metabolism</topic><topic>Hydrolysis</topic><topic>Methylation</topic><topic>Microscopy, Electron</topic><topic>Molecular Weight</topic><topic>Other biological molecules</topic><topic>Polysaccharides, Bacterial</topic><topic>Solubility</topic><topic>Streptococcus</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inoue, Masakazu</creatorcontrib><creatorcontrib>Yakushiji, Tsuyoshi</creatorcontrib><creatorcontrib>Katsuki, Midori</creatorcontrib><creatorcontrib>Kudo, Naoko</creatorcontrib><creatorcontrib>Koga, Toshihiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, Masakazu</au><au>Yakushiji, Tsuyoshi</au><au>Katsuki, Midori</au><au>Kudo, Naoko</au><au>Koga, Toshihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reduction of the adherence of Streptococcus sobrinus insoluble α- d-glucan by endo-(1→3)-α- d-glucanase</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>1988-11-01</date><risdate>1988</risdate><volume>182</volume><issue>2</issue><spage>277</spage><epage>286</epage><pages>277-286</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><coden>CRBRAT</coden><abstract>Insoluble α-
d-glucan, previously formed on a glass surface from sucrose by the action of cell-free
d-glucosyltransferases of
Streptococcus sobrinus OMZ176, was significantly removed by a purified preparation of endo-(1→3)-α-
d-glucanase (mutanase) from a strain of
Pseudomonas sp. Almost complete dissociation of adherent glucan occurred at the highest enzyme concentration (40 mU/mL) tested. Synthesis and
de novo adherence on glass of the glucan was markedly inhibited by the presence of mutanase, even at low concentrations (4 mU/mL or less). When compared to native glucan, the mutanase-modified glucan samples (a) contained lower proportion of
d-(1→3) linkages; (b) showed lower susceptibility to mutanase and higher susceptibility to (1→6)-α-
d-glucanase (dextranase); (c) contained larger amounts of low-molecular-weight fractions; (d) had lower intrinsic viscosities; (e) showed higher
S. sobrinus cell-agglutinating activities; and (f) consisted of looser entwinement of coalescent single-stranded fibrils (a major component) and shorter double-stranded fibrils (a minor one).</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2977294</pmid><doi>10.1016/0008-6215(88)84008-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Carbohydrate research, 1988-11, Vol.182 (2), p.277-286 |
| issn | 0008-6215 1873-426X |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adhesiveness Agglutination Tests Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrates Fundamental and applied biological sciences. Psychology Glucans - metabolism Glucosyltransferases - isolation & purification Glycoside Hydrolases - metabolism Hydrolysis Methylation Microscopy, Electron Molecular Weight Other biological molecules Polysaccharides, Bacterial Solubility Streptococcus Viscosity |
| title | Reduction of the adherence of Streptococcus sobrinus insoluble α- d-glucan by endo-(1→3)-α- d-glucanase |
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