Nucleotide sequence of the invasion plasmid antigen B and C genes ( ipaB and ipaC) of Shigella flexneri
The nucleotide sequence of a 4.8 kilobase (kb) HindIII fragment from pWR100, the virulence plasmid of Shigella flexneri 5, was determined and analysed. This fragment encodes polypeptides b (62 kilodalton, kD) and c (43 kD) which have already been described as two of the four immunogenic polypeptides...
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| Veröffentlicht in: | Microbial pathogenesis 1988-05, Vol.4 (5), p.345-357 |
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| creator | Baudry, Bernadette Kaczorek, Michel Sansonetti, Philippe J. |
| description | The nucleotide sequence of a 4.8 kilobase (kb)
HindIII fragment from pWR100, the virulence plasmid of
Shigella flexneri 5, was determined and analysed. This fragment encodes polypeptides b (62 kilodalton, kD) and c (43 kD) which have already been described as two of the four immunogenic polypeptides of
Shigellae. The nucleotide sequence revealed that in addition to the
ipaB and
ipaC genes encoding polypeptides b and c, a third complete open reading frame was found within the fragment. The gene, named
ippI, encoded a 17 kD polypeptide. The deduced amino acids sequence of polypeptides b and c showed no signal peptide but presence of highly hydrophobic domains compatible with a transmembraneous location. The surprising A and T richness of the three genes as compared with the
Escherichia coli and
Shigella genomes, resulted in a biased codon usage, and raises the question of the origin of the sequences. |
| doi_str_mv | 10.1016/0882-4010(88)90062-9 |
| format | Article |
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HindIII fragment from pWR100, the virulence plasmid of
Shigella flexneri 5, was determined and analysed. This fragment encodes polypeptides b (62 kilodalton, kD) and c (43 kD) which have already been described as two of the four immunogenic polypeptides of
Shigellae. The nucleotide sequence revealed that in addition to the
ipaB and
ipaC genes encoding polypeptides b and c, a third complete open reading frame was found within the fragment. The gene, named
ippI, encoded a 17 kD polypeptide. The deduced amino acids sequence of polypeptides b and c showed no signal peptide but presence of highly hydrophobic domains compatible with a transmembraneous location. The surprising A and T richness of the three genes as compared with the
Escherichia coli and
Shigella genomes, resulted in a biased codon usage, and raises the question of the origin of the sequences.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/0882-4010(88)90062-9</identifier><identifier>PMID: 3071655</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier India Pvt Ltd</publisher><subject>Amino Acid Sequence ; Antigens, Bacterial - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Codon - genetics ; DNA sequence ; DNA, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetics ; Immunoblotting ; invasion genes ; Microbiology ; Molecular Sequence Data ; Peptides - genetics ; Peptides - immunology ; Plasmids ; Restriction Mapping ; S. flexneri ; shigella flexneri ; Shigella flexneri - genetics ; Shigella flexneri - immunology ; Shigella flexneri - pathogenicity ; Virulence</subject><ispartof>Microbial pathogenesis, 1988-05, Vol.4 (5), p.345-357</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-8d2bcc71ab13700da31d7383a19674654ada0c0e2e6f5256656376db3ea4caea3</citedby><cites>FETCH-LOGICAL-c332t-8d2bcc71ab13700da31d7383a19674654ada0c0e2e6f5256656376db3ea4caea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0882-4010(88)90062-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7322987$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3071655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baudry, Bernadette</creatorcontrib><creatorcontrib>Kaczorek, Michel</creatorcontrib><creatorcontrib>Sansonetti, Philippe J.</creatorcontrib><title>Nucleotide sequence of the invasion plasmid antigen B and C genes ( ipaB and ipaC) of Shigella flexneri</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>The nucleotide sequence of a 4.8 kilobase (kb)
HindIII fragment from pWR100, the virulence plasmid of
Shigella flexneri 5, was determined and analysed. This fragment encodes polypeptides b (62 kilodalton, kD) and c (43 kD) which have already been described as two of the four immunogenic polypeptides of
Shigellae. The nucleotide sequence revealed that in addition to the
ipaB and
ipaC genes encoding polypeptides b and c, a third complete open reading frame was found within the fragment. The gene, named
ippI, encoded a 17 kD polypeptide. The deduced amino acids sequence of polypeptides b and c showed no signal peptide but presence of highly hydrophobic domains compatible with a transmembraneous location. The surprising A and T richness of the three genes as compared with the
Escherichia coli and
Shigella genomes, resulted in a biased codon usage, and raises the question of the origin of the sequences.</description><subject>Amino Acid Sequence</subject><subject>Antigens, Bacterial - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Codon - genetics</subject><subject>DNA sequence</subject><subject>DNA, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Immunoblotting</subject><subject>invasion genes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Peptides - genetics</subject><subject>Peptides - immunology</subject><subject>Plasmids</subject><subject>Restriction Mapping</subject><subject>S. flexneri</subject><subject>shigella flexneri</subject><subject>Shigella flexneri - genetics</subject><subject>Shigella flexneri - immunology</subject><subject>Shigella flexneri - pathogenicity</subject><subject>Virulence</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQhi0EWkrhH4DkA0K7h8DYTvxxQYKKL2kFB-BsTe3JrlGaFDtdwb_HoVWPcPLI87zj8fsy9lTASwFCvwJrZdOCgEtrrxyAlo27x1YCnG6EBHufrc7IQ_aolB8A4FrlLtiFAiN0163YzedDGGiaUyRe6OeBxkB86vl8SzyNd1jSNPL9gGWXIsdxTjc08re1inzDa02FX_K0x-NVLTZXi_zrbQWHAXk_0K-RcnrMHvQ4FHpyOtfs-_t33zYfm-svHz5t3lw3QSk5NzbKbQhG4FYoAxBRiWiUVSicNq3uWowIAUiS7jvZad1pZXTcKsI2IKFasxfHufs81d-U2e9SCcsqI02H4o01ShjZ_RcUretkfaGC7REMeSolU-_3Oe0w__YC_BKEX1z2i8veWv83CO-q7Nlp_mG7o3gWnZyv_eenPpaAQ59xDKmcMaOkdHXXNXt9xKiadpco-xLSElJMmcLs45T-vccfdnqihw</recordid><startdate>198805</startdate><enddate>198805</enddate><creator>Baudry, Bernadette</creator><creator>Kaczorek, Michel</creator><creator>Sansonetti, Philippe J.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198805</creationdate><title>Nucleotide sequence of the invasion plasmid antigen B and C genes ( ipaB and ipaC) of Shigella flexneri</title><author>Baudry, Bernadette ; Kaczorek, Michel ; Sansonetti, Philippe J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-8d2bcc71ab13700da31d7383a19674654ada0c0e2e6f5256656376db3ea4caea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Antigens, Bacterial - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Codon - genetics</topic><topic>DNA sequence</topic><topic>DNA, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Immunoblotting</topic><topic>invasion genes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Peptides - genetics</topic><topic>Peptides - immunology</topic><topic>Plasmids</topic><topic>Restriction Mapping</topic><topic>S. flexneri</topic><topic>shigella flexneri</topic><topic>Shigella flexneri - genetics</topic><topic>Shigella flexneri - immunology</topic><topic>Shigella flexneri - pathogenicity</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baudry, Bernadette</creatorcontrib><creatorcontrib>Kaczorek, Michel</creatorcontrib><creatorcontrib>Sansonetti, Philippe J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baudry, Bernadette</au><au>Kaczorek, Michel</au><au>Sansonetti, Philippe J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleotide sequence of the invasion plasmid antigen B and C genes ( ipaB and ipaC) of Shigella flexneri</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>1988-05</date><risdate>1988</risdate><volume>4</volume><issue>5</issue><spage>345</spage><epage>357</epage><pages>345-357</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>The nucleotide sequence of a 4.8 kilobase (kb)
HindIII fragment from pWR100, the virulence plasmid of
Shigella flexneri 5, was determined and analysed. This fragment encodes polypeptides b (62 kilodalton, kD) and c (43 kD) which have already been described as two of the four immunogenic polypeptides of
Shigellae. The nucleotide sequence revealed that in addition to the
ipaB and
ipaC genes encoding polypeptides b and c, a third complete open reading frame was found within the fragment. The gene, named
ippI, encoded a 17 kD polypeptide. The deduced amino acids sequence of polypeptides b and c showed no signal peptide but presence of highly hydrophobic domains compatible with a transmembraneous location. The surprising A and T richness of the three genes as compared with the
Escherichia coli and
Shigella genomes, resulted in a biased codon usage, and raises the question of the origin of the sequences.</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>3071655</pmid><doi>10.1016/0882-4010(88)90062-9</doi><tpages>13</tpages></addata></record> |
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| ispartof | Microbial pathogenesis, 1988-05, Vol.4 (5), p.345-357 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_78731725 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Antigens, Bacterial - genetics Bacteriology Base Sequence Biological and medical sciences Cloning, Molecular Codon - genetics DNA sequence DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Immunoblotting invasion genes Microbiology Molecular Sequence Data Peptides - genetics Peptides - immunology Plasmids Restriction Mapping S. flexneri shigella flexneri Shigella flexneri - genetics Shigella flexneri - immunology Shigella flexneri - pathogenicity Virulence |
| title | Nucleotide sequence of the invasion plasmid antigen B and C genes ( ipaB and ipaC) of Shigella flexneri |
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