Evaluation of the genotoxicity of 10 selected dietary/environmental compounds with the in vitro micronucleus cytokinesis-block assay in an interlaboratory comparison
Complex exposure to xenobiotics is one of the reasons for the reported increase of respiratory diseases, cancer and immunological disturbances. Among such xenobiotics there are food mutagens whose effects on human health in the low level and/or chronic exposure still remains unknown. In the present...
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| creator | Katic, Jelena Cemeli, Eduardo Baumgartner, Adolf Laubenthal, Julian Bassano, Irene Stølevik, Solvor B. Granum, Berit Namork, Ellen Nygaard, Unni C. Løvik, Martinus Leeuwen, Danitsja van Loock, Kim Vande Anderson, Diana Fučić, Aleksandra Decordier, Ilse |
| description | Complex exposure to xenobiotics is one of the reasons for the reported increase of respiratory diseases, cancer and immunological disturbances. Among such xenobiotics there are food mutagens whose effects on human health in the low level and/or chronic exposure still remains unknown. In the present manuscript, the compounds ethanol (EtOH), 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3′,4,4′-tetrachlorobiphenyl (PCB 153), benzo[a]pyrene (BaP), 2-amino-3-methylimidazol[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine (PhIP), N-Nitrosodimethylamine (NDMA) and acrylamide (AA) were evaluated in an interlaboratory comparison in the in vitro cytokinesis-block micronucleus assay (CBMN) with objective of assessing the induction of micronuclei, buds and nucleoplasmic bridges in dose responses. Statistically significant increase in MNBN frequency in binucleated cells was recorded by both laboratories for the compound PhIP (2.5μM). The compounds PCB (250μM) and AA (500μM) induced statistically significant increase of MNBN although it was recorded by one of the two laboratories. Induction of buds and nucleoplasmic bridges was only observed for BaP (100μM) and AA (500μM) by one of the laboratories. Data generated in this study may assist in the interpretation of the mother/newborn biomonitoring study being carried out within project NewGeneris and will contribute to overall knowledge on the genotoxic potential of dietary/environmental toxicants. |
| doi_str_mv | 10.1016/j.fct.2010.06.030 |
| format | Article |
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Among such xenobiotics there are food mutagens whose effects on human health in the low level and/or chronic exposure still remains unknown. In the present manuscript, the compounds ethanol (EtOH), 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3′,4,4′-tetrachlorobiphenyl (PCB 153), benzo[a]pyrene (BaP), 2-amino-3-methylimidazol[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine (PhIP), N-Nitrosodimethylamine (NDMA) and acrylamide (AA) were evaluated in an interlaboratory comparison in the in vitro cytokinesis-block micronucleus assay (CBMN) with objective of assessing the induction of micronuclei, buds and nucleoplasmic bridges in dose responses. Statistically significant increase in MNBN frequency in binucleated cells was recorded by both laboratories for the compound PhIP (2.5μM). The compounds PCB (250μM) and AA (500μM) induced statistically significant increase of MNBN although it was recorded by one of the two laboratories. Induction of buds and nucleoplasmic bridges was only observed for BaP (100μM) and AA (500μM) by one of the laboratories. Data generated in this study may assist in the interpretation of the mother/newborn biomonitoring study being carried out within project NewGeneris and will contribute to overall knowledge on the genotoxic potential of dietary/environmental toxicants.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2010.06.030</identifier><identifier>PMID: 20600534</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Biological and medical sciences ; Cells, Cultured ; Cytokinesis - drug effects ; Dietary genotoxicants ; Environmental Monitoring ; Environmental Pollutants - toxicity ; Female ; Food Analysis ; Humans ; Laboratories ; Male ; Medical sciences ; Micronuclei ; Micronucleus Tests ; Mutagens - toxicity ; Nuclear buds ; Nucleoplasmic bridges ; Quality Control ; Toxicology ; Xenobiotics - toxicity ; Young Adult</subject><ispartof>Food and chemical toxicology, 2010-10, Vol.48 (10), p.2612-2623</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright (c) 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-e4a9365e22276cfccf8f2cf25ab5eac18f10d5536cc9710de37f5d3544284f33</citedby><cites>FETCH-LOGICAL-c457t-e4a9365e22276cfccf8f2cf25ab5eac18f10d5536cc9710de37f5d3544284f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fct.2010.06.030$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23324976$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20600534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katic, Jelena</creatorcontrib><creatorcontrib>Cemeli, Eduardo</creatorcontrib><creatorcontrib>Baumgartner, Adolf</creatorcontrib><creatorcontrib>Laubenthal, Julian</creatorcontrib><creatorcontrib>Bassano, Irene</creatorcontrib><creatorcontrib>Stølevik, Solvor B.</creatorcontrib><creatorcontrib>Granum, Berit</creatorcontrib><creatorcontrib>Namork, Ellen</creatorcontrib><creatorcontrib>Nygaard, Unni C.</creatorcontrib><creatorcontrib>Løvik, Martinus</creatorcontrib><creatorcontrib>Leeuwen, Danitsja van</creatorcontrib><creatorcontrib>Loock, Kim Vande</creatorcontrib><creatorcontrib>Anderson, Diana</creatorcontrib><creatorcontrib>Fučić, Aleksandra</creatorcontrib><creatorcontrib>Decordier, Ilse</creatorcontrib><title>Evaluation of the genotoxicity of 10 selected dietary/environmental compounds with the in vitro micronucleus cytokinesis-block assay in an interlaboratory comparison</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>Complex exposure to xenobiotics is one of the reasons for the reported increase of respiratory diseases, cancer and immunological disturbances. Among such xenobiotics there are food mutagens whose effects on human health in the low level and/or chronic exposure still remains unknown. In the present manuscript, the compounds ethanol (EtOH), 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3′,4,4′-tetrachlorobiphenyl (PCB 153), benzo[a]pyrene (BaP), 2-amino-3-methylimidazol[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine (PhIP), N-Nitrosodimethylamine (NDMA) and acrylamide (AA) were evaluated in an interlaboratory comparison in the in vitro cytokinesis-block micronucleus assay (CBMN) with objective of assessing the induction of micronuclei, buds and nucleoplasmic bridges in dose responses. Statistically significant increase in MNBN frequency in binucleated cells was recorded by both laboratories for the compound PhIP (2.5μM). The compounds PCB (250μM) and AA (500μM) induced statistically significant increase of MNBN although it was recorded by one of the two laboratories. Induction of buds and nucleoplasmic bridges was only observed for BaP (100μM) and AA (500μM) by one of the laboratories. 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Among such xenobiotics there are food mutagens whose effects on human health in the low level and/or chronic exposure still remains unknown. In the present manuscript, the compounds ethanol (EtOH), 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3′,4,4′-tetrachlorobiphenyl (PCB 153), benzo[a]pyrene (BaP), 2-amino-3-methylimidazol[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine (PhIP), N-Nitrosodimethylamine (NDMA) and acrylamide (AA) were evaluated in an interlaboratory comparison in the in vitro cytokinesis-block micronucleus assay (CBMN) with objective of assessing the induction of micronuclei, buds and nucleoplasmic bridges in dose responses. Statistically significant increase in MNBN frequency in binucleated cells was recorded by both laboratories for the compound PhIP (2.5μM). The compounds PCB (250μM) and AA (500μM) induced statistically significant increase of MNBN although it was recorded by one of the two laboratories. Induction of buds and nucleoplasmic bridges was only observed for BaP (100μM) and AA (500μM) by one of the laboratories. Data generated in this study may assist in the interpretation of the mother/newborn biomonitoring study being carried out within project NewGeneris and will contribute to overall knowledge on the genotoxic potential of dietary/environmental toxicants.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>20600534</pmid><doi>10.1016/j.fct.2010.06.030</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Biological and medical sciences Cells, Cultured Cytokinesis - drug effects Dietary genotoxicants Environmental Monitoring Environmental Pollutants - toxicity Female Food Analysis Humans Laboratories Male Medical sciences Micronuclei Micronucleus Tests Mutagens - toxicity Nuclear buds Nucleoplasmic bridges Quality Control Toxicology Xenobiotics - toxicity Young Adult |
| title | Evaluation of the genotoxicity of 10 selected dietary/environmental compounds with the in vitro micronucleus cytokinesis-block assay in an interlaboratory comparison |
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