A novel organotypic tauopathy model on a new microcavity chip for bioelectronic label-free and real time monitoring
Herewith we developed a novel 3D in vitro Alzheimer's disease (AD) model, based on the human neuroblastoma cell line SH-SY5Y, which is well differentiated without the application of any agents. Furthermore AD-like pathological neurodegeneration can be induced by okadaic acid (OA) mediated hyper...
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| Veröffentlicht in: | Biosensors & bioelectronics 2010-09, Vol.26 (1), p.162-168 |
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| creator | Krinke, Dana Jahnke, Heinz-Georg Mack, Till G.A. Hirche, Anika Striggow, Frank Robitzki, Andrea A. |
| description | Herewith we developed a novel 3D
in vitro Alzheimer's disease (AD) model, based on the human neuroblastoma cell line SH-SY5Y, which is well differentiated without the application of any agents. Furthermore AD-like pathological neurodegeneration can be induced by okadaic acid (OA) mediated hyperphosphorylation of the microtubule associated protein tau. Moreover, we established stable “rapid tauopathy cell lines” expressing additional EGFP-fused (enhanced green fluorescent protein) wildtype or a pathology-promoting mutant tau variant (P301L) by lentiviral transduction. For the sensitive and feasible quantitative detection of pathological effects on neuronal 3D-cultures by electrochemical impedance spectroscopy (EIS) we optimized and redesigned a microcavity array (MCA). The cellular contribution to impedance could be increased by the factor of 2.5 and the variance decreased by 40%. Using our optimized MCA and impedance measurement setup we were able to detect quantitatively an OA concentration- and time-dependent decrease of the impedance in 3D SH-SY5Y cultures. Moreover, we were able to detect and quantify distinct, AD-related effects triggered by tau-mutant (P301L) expression and hyperphosphorylation in our organotypic 3D-cultures with the help of impedance spectroscopy. |
| doi_str_mv | 10.1016/j.bios.2010.06.002 |
| format | Article |
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in vitro Alzheimer's disease (AD) model, based on the human neuroblastoma cell line SH-SY5Y, which is well differentiated without the application of any agents. Furthermore AD-like pathological neurodegeneration can be induced by okadaic acid (OA) mediated hyperphosphorylation of the microtubule associated protein tau. Moreover, we established stable “rapid tauopathy cell lines” expressing additional EGFP-fused (enhanced green fluorescent protein) wildtype or a pathology-promoting mutant tau variant (P301L) by lentiviral transduction. For the sensitive and feasible quantitative detection of pathological effects on neuronal 3D-cultures by electrochemical impedance spectroscopy (EIS) we optimized and redesigned a microcavity array (MCA). The cellular contribution to impedance could be increased by the factor of 2.5 and the variance decreased by 40%. Using our optimized MCA and impedance measurement setup we were able to detect quantitatively an OA concentration- and time-dependent decrease of the impedance in 3D SH-SY5Y cultures. Moreover, we were able to detect and quantify distinct, AD-related effects triggered by tau-mutant (P301L) expression and hyperphosphorylation in our organotypic 3D-cultures with the help of impedance spectroscopy.</description><subject>Alzheimer's disease model</subject><subject>Arrays</subject><subject>Bioelectricity</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - instrumentation</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>Cell Line, Tumor</subject><subject>Cellular</subject><subject>Computer Systems</subject><subject>Dielectric Spectroscopy - instrumentation</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Impedance</subject><subject>Impedance spectroscopy</subject><subject>Microarray Analysis - instrumentation</subject><subject>Microcavities</subject><subject>Microcavity array</subject><subject>Miniaturization</subject><subject>Okadaic acid</subject><subject>Proteins</subject><subject>SH-SY5Y cell line</subject><subject>Staining and Labeling</subject><subject>Tau hyperphosphorylation</subject><subject>tau Proteins - metabolism</subject><subject>Tauopathies - metabolism</subject><subject>Tauopathies - pathology</subject><subject>Three dimensional</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EotPCC7BA3iC6yXDt-CeW2FQVf1IlNrC2HNtpPUrsYGemmrfH0QywKytL1989ujofQm8IbAkQ8WG37UMqWwp1AGILQJ-hDelk2zDa8udoA4qLhgvRXqDLUnYAIImCl-iCAldEMLZB5QbHdPAjTvnexLQc52DxYvZpNsvDEU_JrX8RGxz9I56CzcmaQ1iO2D6EGQ8p43qEH71dcop1dzS9H5she49NdDh7M-IlTL5GxbCkHOL9K_RiMGPxr8_vFfr5-dOP26_N3fcv325v7hrLOCwNA-cMV1YIypwcpOwpFdx0nKi-5x13XCrFO0FAsb7vDXOOArND2zFiTK3hCr0_5c45_dr7sugpFOvH0USf9kXLTtJWSQH_JzkDEKxdM6-fJImQhHIFYkXpCa2dlZL9oOccJpOPmoBeBeqdXgXqVaAGoavAuvT2nL_vJ-_-rvwxVoF3Z8AUa8Yhm2hD-ce1VECnVOU-njhfGz4En3WxwUfrXchVlnYpPHXHby0guTg</recordid><startdate>20100915</startdate><enddate>20100915</enddate><creator>Krinke, Dana</creator><creator>Jahnke, Heinz-Georg</creator><creator>Mack, Till G.A.</creator><creator>Hirche, Anika</creator><creator>Striggow, Frank</creator><creator>Robitzki, Andrea A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100915</creationdate><title>A novel organotypic tauopathy model on a new microcavity chip for bioelectronic label-free and real time monitoring</title><author>Krinke, Dana ; Jahnke, Heinz-Georg ; Mack, Till G.A. ; Hirche, Anika ; Striggow, Frank ; Robitzki, Andrea A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-40dda59c6624d7f77b2265a8519bb585d57995861094bbba4dd204cf3841aa873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Alzheimer's disease model</topic><topic>Arrays</topic><topic>Bioelectricity</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - instrumentation</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>Cell Line, Tumor</topic><topic>Cellular</topic><topic>Computer Systems</topic><topic>Dielectric Spectroscopy - instrumentation</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Impedance</topic><topic>Impedance spectroscopy</topic><topic>Microarray Analysis - instrumentation</topic><topic>Microcavities</topic><topic>Microcavity array</topic><topic>Miniaturization</topic><topic>Okadaic acid</topic><topic>Proteins</topic><topic>SH-SY5Y cell line</topic><topic>Staining and Labeling</topic><topic>Tau hyperphosphorylation</topic><topic>tau Proteins - metabolism</topic><topic>Tauopathies - metabolism</topic><topic>Tauopathies - pathology</topic><topic>Three dimensional</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krinke, Dana</creatorcontrib><creatorcontrib>Jahnke, Heinz-Georg</creatorcontrib><creatorcontrib>Mack, Till G.A.</creatorcontrib><creatorcontrib>Hirche, Anika</creatorcontrib><creatorcontrib>Striggow, Frank</creatorcontrib><creatorcontrib>Robitzki, Andrea A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krinke, Dana</au><au>Jahnke, Heinz-Georg</au><au>Mack, Till G.A.</au><au>Hirche, Anika</au><au>Striggow, Frank</au><au>Robitzki, Andrea A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel organotypic tauopathy model on a new microcavity chip for bioelectronic label-free and real time monitoring</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2010-09-15</date><risdate>2010</risdate><volume>26</volume><issue>1</issue><spage>162</spage><epage>168</epage><pages>162-168</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Herewith we developed a novel 3D
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| ispartof | Biosensors & bioelectronics, 2010-09, Vol.26 (1), p.162-168 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alzheimer's disease model Arrays Bioelectricity Biological and medical sciences Biological Assay - instrumentation Biosensing Techniques - instrumentation Biotechnology Cell Line, Tumor Cellular Computer Systems Dielectric Spectroscopy - instrumentation Electrochemical impedance spectroscopy Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Humans Impedance Impedance spectroscopy Microarray Analysis - instrumentation Microcavities Microcavity array Miniaturization Okadaic acid Proteins SH-SY5Y cell line Staining and Labeling Tau hyperphosphorylation tau Proteins - metabolism Tauopathies - metabolism Tauopathies - pathology Three dimensional |
| title | A novel organotypic tauopathy model on a new microcavity chip for bioelectronic label-free and real time monitoring |
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