Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid)

A method for assaying glutathione reductase (GSH; EC 1.6.4.2) in crude plant extracts is described. The method is based on the increase in absorbance at 412 nm when 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) is reduced by GSH. The effects of the following parameters on the assay were tested: various...

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Veröffentlicht in:	Analytical biochemistry 1988-12, Vol.175 (2), p.408-413
Hauptverfasser:	Smith, Ivan K., Vierheller, Thomas L., Thorne, Carol A.
Format:	Artikel
Sprache:	eng
Schlagworte:	5,5'-dithiobis(2-nitrobenzoic acid) Analytical, structural and metabolic biochemistry Biological and medical sciences Dithionitrobenzoic Acid Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology glutathione glutathione reductase Glutathione Reductase - analysis Glycine max Kinetics Oxidoreductases plant systems Plants - enzymology spectrophotometry Spectrophotometry - methods
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