Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound
Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis...
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| Veröffentlicht in: | Biometals 2004-11, Vol.17 (6), p.647-654 |
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| creator | Schrettl, Markus Winkelmann, Günther Haas, Hubertus |
| description | Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-richconditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans. |
| doi_str_mv | 10.1007/s10534-004-1230-z |
| format | Article |
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Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-richconditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans.</description><identifier>ISSN: 0966-0844</identifier><identifier>EISSN: 1572-8773</identifier><identifier>DOI: 10.1007/s10534-004-1230-z</identifier><identifier>PMID: 15689108</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Amino Acid Sequence ; Aspergillus nidulans ; Bioassays ; Biological Assay ; Biological Transport ; Biosynthesis ; Candida albicans ; Chromatography, High Pressure Liquid ; Enzymes ; Ferrichrome - chemistry ; Fungal Proteins ; Hydroxamic Acids - chemistry ; Iron ; Iron - metabolism ; Liquid chromatography ; Mass Spectrometry ; Models, Chemical ; Molecular Sequence Data ; Phylogeny ; Protein Structure, Tertiary ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - metabolism ; Schizosaccharomyces - metabolism ; Schizosaccharomyces - physiology ; Schizosaccharomyces pombe ; Yeast ; Yeasts</subject><ispartof>Biometals, 2004-11, Vol.17 (6), p.647-654</ispartof><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-d9b85bd7d9523c633aba25a43986755c67bb91c9018904a1feae0d29009672173</citedby><cites>FETCH-LOGICAL-c424t-d9b85bd7d9523c633aba25a43986755c67bb91c9018904a1feae0d29009672173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15689108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schrettl, Markus</creatorcontrib><creatorcontrib>Winkelmann, Günther</creatorcontrib><creatorcontrib>Haas, Hubertus</creatorcontrib><title>Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound</title><title>Biometals</title><addtitle>Biometals</addtitle><description>Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-richconditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans.</description><subject>Amino Acid Sequence</subject><subject>Aspergillus nidulans</subject><subject>Bioassays</subject><subject>Biological Assay</subject><subject>Biological Transport</subject><subject>Biosynthesis</subject><subject>Candida albicans</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Enzymes</subject><subject>Ferrichrome - chemistry</subject><subject>Fungal Proteins</subject><subject>Hydroxamic Acids - chemistry</subject><subject>Iron</subject><subject>Iron - metabolism</subject><subject>Liquid chromatography</subject><subject>Mass Spectrometry</subject><subject>Models, Chemical</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Protein Structure, Tertiary</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Schizosaccharomyces - 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Academic</collection><jtitle>Biometals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schrettl, Markus</au><au>Winkelmann, Günther</au><au>Haas, Hubertus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound</atitle><jtitle>Biometals</jtitle><addtitle>Biometals</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>17</volume><issue>6</issue><spage>647</spage><epage>654</epage><pages>647-654</pages><issn>0966-0844</issn><eissn>1572-8773</eissn><abstract>Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-richconditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>15689108</pmid><doi>10.1007/s10534-004-1230-z</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Aspergillus nidulans Bioassays Biological Assay Biological Transport Biosynthesis Candida albicans Chromatography, High Pressure Liquid Enzymes Ferrichrome - chemistry Fungal Proteins Hydroxamic Acids - chemistry Iron Iron - metabolism Liquid chromatography Mass Spectrometry Models, Chemical Molecular Sequence Data Phylogeny Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Schizosaccharomyces - metabolism Schizosaccharomyces - physiology Schizosaccharomyces pombe Yeast Yeasts |
| title | Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound |
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