Trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor
This study determined the effects of Trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic T. vaginalis cultures on human sperm motility and viability. Spent medium (pH 7.0) caused complete cessation of sperm motility after 15 minutes incubation. Trophozoite soluble fract...
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| Veröffentlicht in: | Annals of clinical and laboratory science 1988-11, Vol.18 (6), p.484-489 |
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| container_title | Annals of clinical and laboratory science |
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| creator | JARECKI-BLACK, J. C LUSHBAUGH, W. B GOLOSOV, L GLASSMAN, A. B |
| description | This study determined the effects of Trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic T. vaginalis cultures on human sperm motility and viability. Spent medium (pH 7.0) caused complete cessation of sperm motility after 15 minutes incubation. Trophozoite soluble fraction or formalin-killed trophozoites caused a 50 percent reduction in sperm motility, compared to 25 percent reduction caused by the trophozoite particulate fraction or the sterile medium and three percent by saline (control). Spent medium from T. vaginalis cultures reaching stationary growth phase produced the greatest reduction in sperm motility, suggesting that potency was related to time in culture and trophozoites per ml. The T. vaginalis spermicidal activity was heat-stable, trypsin-sensitive, and had a molecular weight of 12-15,000 by gel filtration. This proteinaceous substance was present in and secreted by T. vaginalis trophozoites during normal growth in axenic culture. Since this T. vaginalis byproduct rapidly killed sperm in vitro, its effects in humans may contribute to infertility in infected couples. |
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C ; LUSHBAUGH, W. B ; GOLOSOV, L ; GLASSMAN, A. B</creator><creatorcontrib>JARECKI-BLACK, J. C ; LUSHBAUGH, W. B ; GOLOSOV, L ; GLASSMAN, A. B</creatorcontrib><description>This study determined the effects of Trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic T. vaginalis cultures on human sperm motility and viability. Spent medium (pH 7.0) caused complete cessation of sperm motility after 15 minutes incubation. Trophozoite soluble fraction or formalin-killed trophozoites caused a 50 percent reduction in sperm motility, compared to 25 percent reduction caused by the trophozoite particulate fraction or the sterile medium and three percent by saline (control). Spent medium from T. vaginalis cultures reaching stationary growth phase produced the greatest reduction in sperm motility, suggesting that potency was related to time in culture and trophozoites per ml. The T. vaginalis spermicidal activity was heat-stable, trypsin-sensitive, and had a molecular weight of 12-15,000 by gel filtration. This proteinaceous substance was present in and secreted by T. vaginalis trophozoites during normal growth in axenic culture. Since this T. vaginalis byproduct rapidly killed sperm in vitro, its effects in humans may contribute to infertility in infected couples.</description><identifier>ISSN: 0091-7370</identifier><identifier>EISSN: 1550-8080</identifier><identifier>PMID: 3266454</identifier><identifier>CODEN: ACLSCP</identifier><language>eng</language><publisher>Philadelphia, PA: Institute for Clinical Science</publisher><subject>Age Factors ; Animals ; Biological and medical sciences ; Chromatography, Gel ; General aspects ; Human infectious diseases. 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C</creatorcontrib><creatorcontrib>LUSHBAUGH, W. B</creatorcontrib><creatorcontrib>GOLOSOV, L</creatorcontrib><creatorcontrib>GLASSMAN, A. B</creatorcontrib><title>Trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor</title><title>Annals of clinical and laboratory science</title><addtitle>Ann Clin Lab Sci</addtitle><description>This study determined the effects of Trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic T. vaginalis cultures on human sperm motility and viability. Spent medium (pH 7.0) caused complete cessation of sperm motility after 15 minutes incubation. Trophozoite soluble fraction or formalin-killed trophozoites caused a 50 percent reduction in sperm motility, compared to 25 percent reduction caused by the trophozoite particulate fraction or the sterile medium and three percent by saline (control). Spent medium from T. vaginalis cultures reaching stationary growth phase produced the greatest reduction in sperm motility, suggesting that potency was related to time in culture and trophozoites per ml. The T. vaginalis spermicidal activity was heat-stable, trypsin-sensitive, and had a molecular weight of 12-15,000 by gel filtration. This proteinaceous substance was present in and secreted by T. vaginalis trophozoites during normal growth in axenic culture. Since this T. vaginalis byproduct rapidly killed sperm in vitro, its effects in humans may contribute to infertility in infected couples.</description><subject>Age Factors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Gel</subject><subject>General aspects</subject><subject>Human infectious diseases. Experimental studies and models</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Sperm Motility - drug effects</subject><subject>Trichomonas vaginalis - analysis</subject><subject>Trichomonas vaginalis - growth & development</subject><subject>Trypsin Inhibitors - pharmacology</subject><issn>0091-7370</issn><issn>1550-8080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAURYMo4zj6E4QsxF0haZqkcSeDXzDgZly4Kq-ZdPqkbWrSEcZfb8Di1tW7l3N4i3tCllxKlpWsZKdkyZjhmRaanZOLGD8Yy01RsAVZiFypQhZL8r4NaFvf-wEi_YI9DtBhvKNjcB32qYUjtS0EsJML-A0T-oH6hgKNows97f2EHU5HikOLNU447GmTZB8uyVkDXXRX812Rt8eH7fo527w-vazvN1mbGzVlpna2MYWuucqttADARGr5rsitqjlYUxpRClEnUgvR6LJUSihX7JxmkOKK3P7-HYP_PLg4VT1G67oOBucPsdKl5koy_q_IpWCCG5HE61k81L3bVWPAPu1QzaMlfjNziBa6JsBgMf5pmkmpmBI_2o53kg</recordid><startdate>19881101</startdate><enddate>19881101</enddate><creator>JARECKI-BLACK, J. C</creator><creator>LUSHBAUGH, W. B</creator><creator>GOLOSOV, L</creator><creator>GLASSMAN, A. B</creator><general>Institute for Clinical Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19881101</creationdate><title>Trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor</title><author>JARECKI-BLACK, J. C ; LUSHBAUGH, W. B ; GOLOSOV, L ; GLASSMAN, A. B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h296t-9becf947b162c5caaa0347b2d42c6b1ac9893833baaab33f7886636e4de70a663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Age Factors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Gel</topic><topic>General aspects</topic><topic>Human infectious diseases. Experimental studies and models</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Sperm Motility - drug effects</topic><topic>Trichomonas vaginalis - analysis</topic><topic>Trichomonas vaginalis - growth & development</topic><topic>Trypsin Inhibitors - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JARECKI-BLACK, J. C</creatorcontrib><creatorcontrib>LUSHBAUGH, W. B</creatorcontrib><creatorcontrib>GOLOSOV, L</creatorcontrib><creatorcontrib>GLASSMAN, A. B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical and laboratory science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JARECKI-BLACK, J. C</au><au>LUSHBAUGH, W. B</au><au>GOLOSOV, L</au><au>GLASSMAN, A. B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor</atitle><jtitle>Annals of clinical and laboratory science</jtitle><addtitle>Ann Clin Lab Sci</addtitle><date>1988-11-01</date><risdate>1988</risdate><volume>18</volume><issue>6</issue><spage>484</spage><epage>489</epage><pages>484-489</pages><issn>0091-7370</issn><eissn>1550-8080</eissn><coden>ACLSCP</coden><abstract>This study determined the effects of Trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic T. vaginalis cultures on human sperm motility and viability. Spent medium (pH 7.0) caused complete cessation of sperm motility after 15 minutes incubation. Trophozoite soluble fraction or formalin-killed trophozoites caused a 50 percent reduction in sperm motility, compared to 25 percent reduction caused by the trophozoite particulate fraction or the sterile medium and three percent by saline (control). Spent medium from T. vaginalis cultures reaching stationary growth phase produced the greatest reduction in sperm motility, suggesting that potency was related to time in culture and trophozoites per ml. The T. vaginalis spermicidal activity was heat-stable, trypsin-sensitive, and had a molecular weight of 12-15,000 by gel filtration. This proteinaceous substance was present in and secreted by T. vaginalis trophozoites during normal growth in axenic culture. Since this T. vaginalis byproduct rapidly killed sperm in vitro, its effects in humans may contribute to infertility in infected couples.</abstract><cop>Philadelphia, PA</cop><pub>Institute for Clinical Science</pub><pmid>3266454</pmid><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0091-7370 |
| ispartof | Annals of clinical and laboratory science, 1988-11, Vol.18 (6), p.484-489 |
| issn | 0091-7370 1550-8080 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Age Factors Animals Biological and medical sciences Chromatography, Gel General aspects Human infectious diseases. Experimental studies and models Humans In Vitro Techniques Infectious diseases Male Medical sciences Sperm Motility - drug effects Trichomonas vaginalis - analysis Trichomonas vaginalis - growth & development Trypsin Inhibitors - pharmacology |
| title | Trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor |
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