Effect of serine proteinase from Staphylococcus aureus on in vitro stimulation of human lymphocytes
In a broad concentration range (0.1–100 μg/ml) the serine proteinase (SP) from Staphylococcus aureus has no cytotoxic effect on human peripheral blood lymphocytes and does not stimulate them in culture. However, it affects the action of a number of polyclonal activators. In a concentration of 100 μg...
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| Veröffentlicht in: | Immunology letters 1988-10, Vol.19 (2), p.127-132 |
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| container_title | Immunology letters |
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| creator | Prokešová, Ludmila Porwit-Bóbr, Zofia Baran, Krystyna Potempa, Jan John, Ctirad |
| description | In a broad concentration range (0.1–100 μg/ml) the serine proteinase (SP) from
Staphylococcus aureus has no cytotoxic effect on human peripheral blood lymphocytes and does not stimulate them in culture. However, it affects the action of a number of polyclonal activators. In a concentration of 100 μg/ml SP completely eliminates blastic transformation after stimulation with B cell mitogens (NDCM,
S. aureus and
Escherichia coli), lowers the blastic transformation after stimulation with PWM and SPA, and does not affect the blastic transformation after stimulation with PHA. SP (100 μg/ml) reduces the concentration of Ig in stimulated cultures (stimulation with PWM, NDCM,
S. aureus and
E. coli) far below the Ig level of unstimulated controls. This effect can be ascribed to an influence on cell stimulation, not to the proteolytic cleavage of secreted Ig, although SP can partially digest Ig. The effect on lymphocyte stimulation takes place when the SP is added to the culture together with the mitogen, or 18 h after the mitogen. This implies that SP does not affect the first stage of stimulation. |
| doi_str_mv | 10.1016/0165-2478(88)90131-9 |
| format | Article |
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Staphylococcus aureus has no cytotoxic effect on human peripheral blood lymphocytes and does not stimulate them in culture. However, it affects the action of a number of polyclonal activators. In a concentration of 100 μg/ml SP completely eliminates blastic transformation after stimulation with B cell mitogens (NDCM,
S. aureus and
Escherichia coli), lowers the blastic transformation after stimulation with PWM and SPA, and does not affect the blastic transformation after stimulation with PHA. SP (100 μg/ml) reduces the concentration of Ig in stimulated cultures (stimulation with PWM, NDCM,
S. aureus and
E. coli) far below the Ig level of unstimulated controls. This effect can be ascribed to an influence on cell stimulation, not to the proteolytic cleavage of secreted Ig, although SP can partially digest Ig. The effect on lymphocyte stimulation takes place when the SP is added to the culture together with the mitogen, or 18 h after the mitogen. This implies that SP does not affect the first stage of stimulation.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>DOI: 10.1016/0165-2478(88)90131-9</identifier><identifier>PMID: 3235112</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Humans ; Immunoglobulins - metabolism ; In Vitro Techniques ; Lymphocyte Activation - drug effects ; Lymphocyte stimulation ; Lymphocytes - drug effects ; Lymphocytes - immunology ; Mitogens - pharmacology ; Serine Endopeptidases - pharmacology ; Serine proteinase ; Staphylococcus aureus ; Staphylococcus aureus - enzymology</subject><ispartof>Immunology letters, 1988-10, Vol.19 (2), p.127-132</ispartof><rights>1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-ee9a6eff3095925cb26f8ae6919f35bea2ba9745735139f4ff4c62216e1aac213</citedby><cites>FETCH-LOGICAL-c303t-ee9a6eff3095925cb26f8ae6919f35bea2ba9745735139f4ff4c62216e1aac213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0165247888901319$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3235112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prokešová, Ludmila</creatorcontrib><creatorcontrib>Porwit-Bóbr, Zofia</creatorcontrib><creatorcontrib>Baran, Krystyna</creatorcontrib><creatorcontrib>Potempa, Jan</creatorcontrib><creatorcontrib>John, Ctirad</creatorcontrib><title>Effect of serine proteinase from Staphylococcus aureus on in vitro stimulation of human lymphocytes</title><title>Immunology letters</title><addtitle>Immunol Lett</addtitle><description>In a broad concentration range (0.1–100 μg/ml) the serine proteinase (SP) from
Staphylococcus aureus has no cytotoxic effect on human peripheral blood lymphocytes and does not stimulate them in culture. However, it affects the action of a number of polyclonal activators. In a concentration of 100 μg/ml SP completely eliminates blastic transformation after stimulation with B cell mitogens (NDCM,
S. aureus and
Escherichia coli), lowers the blastic transformation after stimulation with PWM and SPA, and does not affect the blastic transformation after stimulation with PHA. SP (100 μg/ml) reduces the concentration of Ig in stimulated cultures (stimulation with PWM, NDCM,
S. aureus and
E. coli) far below the Ig level of unstimulated controls. This effect can be ascribed to an influence on cell stimulation, not to the proteolytic cleavage of secreted Ig, although SP can partially digest Ig. The effect on lymphocyte stimulation takes place when the SP is added to the culture together with the mitogen, or 18 h after the mitogen. This implies that SP does not affect the first stage of stimulation.</description><subject>Adult</subject><subject>Humans</subject><subject>Immunoglobulins - metabolism</subject><subject>In Vitro Techniques</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte stimulation</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - immunology</subject><subject>Mitogens - pharmacology</subject><subject>Serine Endopeptidases - pharmacology</subject><subject>Serine proteinase</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - enzymology</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcFq3DAQFaEh3W7yBynoVNKDE41k2dalUJa0CQRySHIWWu2IVbGtrSQH_PfVZpccm8MwMPPmzcx7hFwCuwYGzU0JWfG67a667rtiIKBSJ2QBXasqJmv-iSzeIZ_Jl5T-MAZS1OKMnAkuJABfEHvrHNpMg6MJox-R7mLI6EeTkLoYBvqUzW4798EGa6dEzRSxpDBSP9JXn2OgKfth6k32pVh4ttNgRtrPw24b7JwxnZNTZ_qEF8e8JC-_bp9Xd9XD4-_71c-HygomcoWoTIPOCaak4tKueeM6g40C5YRco-Fro9patuV0oVztXG0bzqFBMMZyEEvy7cBbXvg7Ycp68Mli35sRw5R027UgGy4-BIIELmreFGB9ANoYUoro9C76wcRZA9N7E_ReYb1XWHedfjNBqzL29cg_rQfcvA8dVS_9H4c-FjVePUadrMfR4sbHYobeBP__Bf8AOXSXmQ</recordid><startdate>198810</startdate><enddate>198810</enddate><creator>Prokešová, Ludmila</creator><creator>Porwit-Bóbr, Zofia</creator><creator>Baran, Krystyna</creator><creator>Potempa, Jan</creator><creator>John, Ctirad</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198810</creationdate><title>Effect of serine proteinase from Staphylococcus aureus on in vitro stimulation of human lymphocytes</title><author>Prokešová, Ludmila ; Porwit-Bóbr, Zofia ; Baran, Krystyna ; Potempa, Jan ; John, Ctirad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-ee9a6eff3095925cb26f8ae6919f35bea2ba9745735139f4ff4c62216e1aac213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adult</topic><topic>Humans</topic><topic>Immunoglobulins - metabolism</topic><topic>In Vitro Techniques</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte stimulation</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - immunology</topic><topic>Mitogens - pharmacology</topic><topic>Serine Endopeptidases - pharmacology</topic><topic>Serine proteinase</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prokešová, Ludmila</creatorcontrib><creatorcontrib>Porwit-Bóbr, Zofia</creatorcontrib><creatorcontrib>Baran, Krystyna</creatorcontrib><creatorcontrib>Potempa, Jan</creatorcontrib><creatorcontrib>John, Ctirad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prokešová, Ludmila</au><au>Porwit-Bóbr, Zofia</au><au>Baran, Krystyna</au><au>Potempa, Jan</au><au>John, Ctirad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of serine proteinase from Staphylococcus aureus on in vitro stimulation of human lymphocytes</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>1988-10</date><risdate>1988</risdate><volume>19</volume><issue>2</issue><spage>127</spage><epage>132</epage><pages>127-132</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><abstract>In a broad concentration range (0.1–100 μg/ml) the serine proteinase (SP) from
Staphylococcus aureus has no cytotoxic effect on human peripheral blood lymphocytes and does not stimulate them in culture. However, it affects the action of a number of polyclonal activators. In a concentration of 100 μg/ml SP completely eliminates blastic transformation after stimulation with B cell mitogens (NDCM,
S. aureus and
Escherichia coli), lowers the blastic transformation after stimulation with PWM and SPA, and does not affect the blastic transformation after stimulation with PHA. SP (100 μg/ml) reduces the concentration of Ig in stimulated cultures (stimulation with PWM, NDCM,
S. aureus and
E. coli) far below the Ig level of unstimulated controls. This effect can be ascribed to an influence on cell stimulation, not to the proteolytic cleavage of secreted Ig, although SP can partially digest Ig. The effect on lymphocyte stimulation takes place when the SP is added to the culture together with the mitogen, or 18 h after the mitogen. This implies that SP does not affect the first stage of stimulation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>3235112</pmid><doi>10.1016/0165-2478(88)90131-9</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2478 |
| ispartof | Immunology letters, 1988-10, Vol.19 (2), p.127-132 |
| issn | 0165-2478 1879-0542 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Humans Immunoglobulins - metabolism In Vitro Techniques Lymphocyte Activation - drug effects Lymphocyte stimulation Lymphocytes - drug effects Lymphocytes - immunology Mitogens - pharmacology Serine Endopeptidases - pharmacology Serine proteinase Staphylococcus aureus Staphylococcus aureus - enzymology |
| title | Effect of serine proteinase from Staphylococcus aureus on in vitro stimulation of human lymphocytes |
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