The potential for the noninvasive delivery of polymeric nanocarriers using propellant-based inhalers in the treatment of Chlamydial respiratory infections
Abstract A novel strategy for pulmonary delivery of polymeric nanocarriers (NCs) pressurized-metered dose inhalers (pMDIs) is reported in this work. Core–shell particles consisting of a water soluble, hydrofluoroalkane(HFA)-philic biodegradable copolymer of chitosan and poly(lactic acid), and a core...
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| Veröffentlicht in: | Biomaterials 2010-10, Vol.31 (28), p.7376-7385 |
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| creator | Bharatwaj, Balaji Wu, Libo Whittum-Hudson, Judith A da Rocha, Sandro R.P |
| description | Abstract A novel strategy for pulmonary delivery of polymeric nanocarriers (NCs) pressurized-metered dose inhalers (pMDIs) is reported in this work. Core–shell particles consisting of a water soluble, hydrofluoroalkane(HFA)-philic biodegradable copolymer of chitosan and poly(lactic acid), and a core of poly( d , l -lactide-co-glycolide) (PLGA) NCs were prepared by a modified emulsification–diffusion methodology. Dispersions of the core–shell particles in HFA propellant revealed enhanced physical stability compared to polymeric NCs alone, and more importantly, excellent aerosol characteristics as determined by inertial impaction studies. Confocal microscopy revealed that the polymeric NCs from such core–shell particles are capable not only to be taken up by Calu-3 (airway epithelial) cells that have been infected with Chlamydia pneumoniae , an intracellular pathogen, but are also internalized within chlamydial inclusions. Our results suggest that the proposed methodology can be used as a general platform for the delivery of polymeric NCs to the respiratory tract using the inexpensive pMDIs, and that such an approach may be used to target and deliver drugs to treat chlamydial-related infections. |
| doi_str_mv | 10.1016/j.biomaterials.2010.06.005 |
| format | Article |
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Core–shell particles consisting of a water soluble, hydrofluoroalkane(HFA)-philic biodegradable copolymer of chitosan and poly(lactic acid), and a core of poly( d , l -lactide-co-glycolide) (PLGA) NCs were prepared by a modified emulsification–diffusion methodology. Dispersions of the core–shell particles in HFA propellant revealed enhanced physical stability compared to polymeric NCs alone, and more importantly, excellent aerosol characteristics as determined by inertial impaction studies. Confocal microscopy revealed that the polymeric NCs from such core–shell particles are capable not only to be taken up by Calu-3 (airway epithelial) cells that have been infected with Chlamydia pneumoniae , an intracellular pathogen, but are also internalized within chlamydial inclusions. Our results suggest that the proposed methodology can be used as a general platform for the delivery of polymeric NCs to the respiratory tract using the inexpensive pMDIs, and that such an approach may be used to target and deliver drugs to treat chlamydial-related infections.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2010.06.005</identifier><identifier>PMID: 20615546</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Aerosol Propellants - chemistry ; Aerosols ; Anti-Bacterial Agents - administration & dosage ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - therapeutic use ; Cell Line ; Chlamydia - metabolism ; Chlamydia Infections - drug therapy ; Chlamydia pneumoniae ; Chlamydophila pneumoniae ; Coumarins - chemistry ; Dentistry ; Drug Carriers - chemistry ; Drug Carriers - metabolism ; Fluorescent Dyes - chemistry ; Hydrocarbons, Fluorinated - chemistry ; Lactic Acid - chemistry ; Metered Dose Inhalers ; Nanocarriers ; Nanomedicine ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Polyesters ; Polymeric ; Polymers - chemistry ; Polymers - metabolism ; Pressurized-metered-dose inhalers ; Pulmonary drug delivery</subject><ispartof>Biomaterials, 2010-10, Vol.31 (28), p.7376-7385</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-63097f7e65d5add3ad62781b5dde44c23588191a5ef931deec5f2aadb6c9daba3</citedby><cites>FETCH-LOGICAL-c466t-63097f7e65d5add3ad62781b5dde44c23588191a5ef931deec5f2aadb6c9daba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2010.06.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20615546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bharatwaj, Balaji</creatorcontrib><creatorcontrib>Wu, Libo</creatorcontrib><creatorcontrib>Whittum-Hudson, Judith A</creatorcontrib><creatorcontrib>da Rocha, Sandro R.P</creatorcontrib><title>The potential for the noninvasive delivery of polymeric nanocarriers using propellant-based inhalers in the treatment of Chlamydial respiratory infections</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract A novel strategy for pulmonary delivery of polymeric nanocarriers (NCs) pressurized-metered dose inhalers (pMDIs) is reported in this work. Core–shell particles consisting of a water soluble, hydrofluoroalkane(HFA)-philic biodegradable copolymer of chitosan and poly(lactic acid), and a core of poly( d , l -lactide-co-glycolide) (PLGA) NCs were prepared by a modified emulsification–diffusion methodology. Dispersions of the core–shell particles in HFA propellant revealed enhanced physical stability compared to polymeric NCs alone, and more importantly, excellent aerosol characteristics as determined by inertial impaction studies. Confocal microscopy revealed that the polymeric NCs from such core–shell particles are capable not only to be taken up by Calu-3 (airway epithelial) cells that have been infected with Chlamydia pneumoniae , an intracellular pathogen, but are also internalized within chlamydial inclusions. Our results suggest that the proposed methodology can be used as a general platform for the delivery of polymeric NCs to the respiratory tract using the inexpensive pMDIs, and that such an approach may be used to target and deliver drugs to treat chlamydial-related infections.</description><subject>Advanced Basic Science</subject><subject>Aerosol Propellants - chemistry</subject><subject>Aerosols</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Cell Line</subject><subject>Chlamydia - metabolism</subject><subject>Chlamydia Infections - drug therapy</subject><subject>Chlamydia pneumoniae</subject><subject>Chlamydophila pneumoniae</subject><subject>Coumarins - chemistry</subject><subject>Dentistry</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - metabolism</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Hydrocarbons, Fluorinated - chemistry</subject><subject>Lactic Acid - chemistry</subject><subject>Metered Dose Inhalers</subject><subject>Nanocarriers</subject><subject>Nanomedicine</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Polyesters</subject><subject>Polymeric</subject><subject>Polymers - chemistry</subject><subject>Polymers - metabolism</subject><subject>Pressurized-metered-dose inhalers</subject><subject>Pulmonary drug delivery</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUsGO1DAMjRCIHRZ-AVVcOHVI2iRtOSChAXaRVuLAco7cxGUytMmQpCP1V_ha0p0FIS5wshI_v2f7mZAXjG4ZZfLVYdtbP0HCYGGM24rmBJVbSsUDsmFt05aio-Ih2VDGq7KTrLogT2I80PymvHpMLioqmRBcbsiP2z0WR5_QpUxWDD4UKf8476w7QbQnLAyOOYSl8ENGjsuUdXXhwHkNIVgMsZijdV-LY_BHHEdwqewhoims28O45q27Y00BIU1ZaqXa7UeYFrOqBoxHGyD5LGLdgDpZ7-JT8mjI8-Gz-3hJvnx4f7u7Lm8-XX3cvb0pNZcylbKmXTM0KIURYEwNRlZNy3phDHKuq1q0LesYCBy6mhlELYYKwPRSdwZ6qC_JyzNv7v_7jDGpyUZ9Nwj6OaqmbfK6as7_jeRtV3HBREa-PiN18DEGHNQx2AnCohhVq4nqoP40Ua0mKipVNjEXP7-XmfsJze_SX65lwLszAPNaTtkBFbVFp9HYkJenjLf_p_PmLxo9Wmc1jN9wwXjwc3BrDVOxUlR9Xs9pvSZGKW3qhtc_AfBVzyo</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Bharatwaj, Balaji</creator><creator>Wu, Libo</creator><creator>Whittum-Hudson, Judith A</creator><creator>da Rocha, Sandro R.P</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20101001</creationdate><title>The potential for the noninvasive delivery of polymeric nanocarriers using propellant-based inhalers in the treatment of Chlamydial respiratory infections</title><author>Bharatwaj, Balaji ; Wu, Libo ; Whittum-Hudson, Judith A ; da Rocha, Sandro R.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-63097f7e65d5add3ad62781b5dde44c23588191a5ef931deec5f2aadb6c9daba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Advanced Basic Science</topic><topic>Aerosol Propellants - chemistry</topic><topic>Aerosols</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Cell Line</topic><topic>Chlamydia - metabolism</topic><topic>Chlamydia Infections - drug therapy</topic><topic>Chlamydia pneumoniae</topic><topic>Chlamydophila pneumoniae</topic><topic>Coumarins - chemistry</topic><topic>Dentistry</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Carriers - metabolism</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Hydrocarbons, Fluorinated - chemistry</topic><topic>Lactic Acid - chemistry</topic><topic>Metered Dose Inhalers</topic><topic>Nanocarriers</topic><topic>Nanomedicine</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - ultrastructure</topic><topic>Polyesters</topic><topic>Polymeric</topic><topic>Polymers - chemistry</topic><topic>Polymers - metabolism</topic><topic>Pressurized-metered-dose inhalers</topic><topic>Pulmonary drug delivery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bharatwaj, Balaji</creatorcontrib><creatorcontrib>Wu, Libo</creatorcontrib><creatorcontrib>Whittum-Hudson, Judith A</creatorcontrib><creatorcontrib>da Rocha, Sandro R.P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bharatwaj, Balaji</au><au>Wu, Libo</au><au>Whittum-Hudson, Judith A</au><au>da Rocha, Sandro R.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The potential for the noninvasive delivery of polymeric nanocarriers using propellant-based inhalers in the treatment of Chlamydial respiratory infections</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>31</volume><issue>28</issue><spage>7376</spage><epage>7385</epage><pages>7376-7385</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract A novel strategy for pulmonary delivery of polymeric nanocarriers (NCs) pressurized-metered dose inhalers (pMDIs) is reported in this work. Core–shell particles consisting of a water soluble, hydrofluoroalkane(HFA)-philic biodegradable copolymer of chitosan and poly(lactic acid), and a core of poly( d , l -lactide-co-glycolide) (PLGA) NCs were prepared by a modified emulsification–diffusion methodology. Dispersions of the core–shell particles in HFA propellant revealed enhanced physical stability compared to polymeric NCs alone, and more importantly, excellent aerosol characteristics as determined by inertial impaction studies. Confocal microscopy revealed that the polymeric NCs from such core–shell particles are capable not only to be taken up by Calu-3 (airway epithelial) cells that have been infected with Chlamydia pneumoniae , an intracellular pathogen, but are also internalized within chlamydial inclusions. Our results suggest that the proposed methodology can be used as a general platform for the delivery of polymeric NCs to the respiratory tract using the inexpensive pMDIs, and that such an approach may be used to target and deliver drugs to treat chlamydial-related infections.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>20615546</pmid><doi>10.1016/j.biomaterials.2010.06.005</doi><tpages>10</tpages></addata></record> |
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| subjects | Advanced Basic Science Aerosol Propellants - chemistry Aerosols Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - therapeutic use Cell Line Chlamydia - metabolism Chlamydia Infections - drug therapy Chlamydia pneumoniae Chlamydophila pneumoniae Coumarins - chemistry Dentistry Drug Carriers - chemistry Drug Carriers - metabolism Fluorescent Dyes - chemistry Hydrocarbons, Fluorinated - chemistry Lactic Acid - chemistry Metered Dose Inhalers Nanocarriers Nanomedicine Nanoparticles - chemistry Nanoparticles - ultrastructure Polyesters Polymeric Polymers - chemistry Polymers - metabolism Pressurized-metered-dose inhalers Pulmonary drug delivery |
| title | The potential for the noninvasive delivery of polymeric nanocarriers using propellant-based inhalers in the treatment of Chlamydial respiratory infections |
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