HER-2/neu cytoplasmic staining is correlated with neuroendocrine differentiation in breast carcinoma
HER2 oncoprotein plays an essential role inbreast cancer growth and differentiation.Determination of HER2 status contributes not onlyto predicting survival but also to selecting thepatients for anti-HER2 therapy. HER2 proteinexpressed in human cancer cells often containsvariant forms as well as the...
Gespeichert in:
| Veröffentlicht in: | Journal of Medical and Dental Sciences 2010, Vol.57(2), pp.155-163 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 163 |
|---|---|
| container_issue | 2 |
| container_start_page | 155 |
| container_title | Journal of Medical and Dental Sciences |
| container_volume | 57 |
| creator | Horiguchi, Shin-ichiro Hishima, Tsunekazu Hayashi, Yukiko Shiozawa, Yumiko Horiguchi, Kazumi Kuroi, Katsumasa Toi, Masakazu Funata, Nobuaki Eishi, Yoshinobu |
| description | HER2 oncoprotein plays an essential role inbreast cancer growth and differentiation.Determination of HER2 status contributes not onlyto predicting survival but also to selecting thepatients for anti-HER2 therapy. HER2 proteinexpressed in human cancer cells often containsvariant forms as well as the full-length wild-typeform. In the present study, we investigated thesubcellular localization of HER2 protein in 1053primary breast cancer tissues. HER2 protein wasstained by various immunohistochemical methodsand studied by immunoelectron microscopy toconfirm the intracellular localization.Thirty-four of 1053 specimens showed cytoplasmicstaining of the intracellular domain of HER2protein by the HercepTest® and CB-11. In contrast,no immunoreactivity to the antibodiesagainst the extracellular domain was observed.None of the 34 specimens showed amplification ofthe HER2 protein by fluorescence in situ hybridization.Subsequently, we studied the associationof the cytoplasmic expression of HER2 with neuroendocrinedifferentiation. Interestingly, all 34specimens had some positive signals of neuroendocrinemarkers such as synaptophysin, chromogranin A, neuron-specific enolase, and CD56.Although the result is preliminary, it warrants furtherstudy on the role of the cytoplasmic variantform of HER2 in breast cancer growth, particularlyin the aspect of neuroendocrine differentiation. |
| doi_str_mv | 10.11480/jmds.570205 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_787048138</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>787048138</sourcerecordid><originalsourceid>FETCH-LOGICAL-j317t-b21fdc31dcfccb137f7bfa32f0b05af9f4fbe21cd1c58c661368bec59e14d2483</originalsourceid><addsrcrecordid>eNo90MFKJDEQgOGwrKyDetvzkpun1lTSmWSOIq4uCILouUkqFc3QnR6TDOLbO8O4XqoO9VGHn7HfIC4Aeisu11OoF9oIKfQPtpBgdbcCJX-yBahedtaCOGZntSYvpAJtQK9-sWMJwqgdWLBwd_PYyctMW44fbd6Mrk4JeW0u5ZRfeKoc51JodI0Cf0_tle9smSmHGUvKxEOKkQrlllxLc-Ypc1_I1cbRFUx5ntwpO4purHT2tU_Y89-bp-u77v7h9t_11X23VmBa5yXEgAoCRkQPykTjo1MyCi-0i6vYR08SMABqi8slqKX1hHpF0AfZW3XCzg9_N2V-21Jtw5Qq0ji6TPO2DsYa0VtQe_nnS279RGHYlDS58jH8D7MDVwew3qV4oW_gSks40rDvPmgzyP049P--4asrA2X1CRv7f8k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>787048138</pqid></control><display><type>article</type><title>HER-2/neu cytoplasmic staining is correlated with neuroendocrine differentiation in breast carcinoma</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese</source><source>Open Access Titles of Japan</source><source>Alma/SFX Local Collection</source><creator>Horiguchi, Shin-ichiro ; Hishima, Tsunekazu ; Hayashi, Yukiko ; Shiozawa, Yumiko ; Horiguchi, Kazumi ; Kuroi, Katsumasa ; Toi, Masakazu ; Funata, Nobuaki ; Eishi, Yoshinobu</creator><creatorcontrib>Horiguchi, Shin-ichiro ; Hishima, Tsunekazu ; Hayashi, Yukiko ; Shiozawa, Yumiko ; Horiguchi, Kazumi ; Kuroi, Katsumasa ; Toi, Masakazu ; Funata, Nobuaki ; Eishi, Yoshinobu</creatorcontrib><description>HER2 oncoprotein plays an essential role inbreast cancer growth and differentiation.Determination of HER2 status contributes not onlyto predicting survival but also to selecting thepatients for anti-HER2 therapy. HER2 proteinexpressed in human cancer cells often containsvariant forms as well as the full-length wild-typeform. In the present study, we investigated thesubcellular localization of HER2 protein in 1053primary breast cancer tissues. HER2 protein wasstained by various immunohistochemical methodsand studied by immunoelectron microscopy toconfirm the intracellular localization.Thirty-four of 1053 specimens showed cytoplasmicstaining of the intracellular domain of HER2protein by the HercepTest® and CB-11. In contrast,no immunoreactivity to the antibodiesagainst the extracellular domain was observed.None of the 34 specimens showed amplification ofthe HER2 protein by fluorescence in situ hybridization.Subsequently, we studied the associationof the cytoplasmic expression of HER2 with neuroendocrinedifferentiation. Interestingly, all 34specimens had some positive signals of neuroendocrinemarkers such as synaptophysin, chromogranin A, neuron-specific enolase, and CD56.Although the result is preliminary, it warrants furtherstudy on the role of the cytoplasmic variantform of HER2 in breast cancer growth, particularlyin the aspect of neuroendocrine differentiation.</description><identifier>ISSN: 1342-8810</identifier><identifier>EISSN: 2185-9132</identifier><identifier>DOI: 10.11480/jmds.570205</identifier><identifier>PMID: 21073134</identifier><language>eng</language><publisher>Japan: Tokyo Medical and Dental University (TMDU)</publisher><subject>Adenocarcinoma, Mucinous - chemistry ; Adenocarcinoma, Mucinous - pathology ; Adult ; Aged ; Aged, 80 and over ; breast cancer ; Breast Neoplasms - chemistry ; Breast Neoplasms - pathology ; Carcinoma, Ductal, Breast - chemistry ; Carcinoma, Ductal, Breast - pathology ; Carcinoma, Lobular - chemistry ; Carcinoma, Lobular - pathology ; CD56 Antigen - analysis ; Cell Differentiation ; Chromogranin A - analysis ; Coloring Agents ; Cytoplasm - chemistry ; cytoplasmic staining ; Dentistry ; Female ; HER2/neu ; Humans ; Immunoenzyme Techniques ; immunohistochemistry ; In Situ Hybridization, Fluorescence ; Middle Aged ; Neuroendocrine Cells - pathology ; neuroendocrine differentiation ; Phosphopyruvate Hydratase - analysis ; Receptor, ErbB-2 - analysis ; Receptor, ErbB-2 - metabolism ; Synaptophysin - analysis ; Young Adult</subject><ispartof>Journal of Medical and Dental Sciences, 2010, Vol.57(2), pp.155-163</ispartof><rights>2010 Tokyo Medical and Dental University (TMDU)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21073134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Horiguchi, Shin-ichiro</creatorcontrib><creatorcontrib>Hishima, Tsunekazu</creatorcontrib><creatorcontrib>Hayashi, Yukiko</creatorcontrib><creatorcontrib>Shiozawa, Yumiko</creatorcontrib><creatorcontrib>Horiguchi, Kazumi</creatorcontrib><creatorcontrib>Kuroi, Katsumasa</creatorcontrib><creatorcontrib>Toi, Masakazu</creatorcontrib><creatorcontrib>Funata, Nobuaki</creatorcontrib><creatorcontrib>Eishi, Yoshinobu</creatorcontrib><title>HER-2/neu cytoplasmic staining is correlated with neuroendocrine differentiation in breast carcinoma</title><title>Journal of Medical and Dental Sciences</title><addtitle>J. med. dent. sci.</addtitle><description>HER2 oncoprotein plays an essential role inbreast cancer growth and differentiation.Determination of HER2 status contributes not onlyto predicting survival but also to selecting thepatients for anti-HER2 therapy. HER2 proteinexpressed in human cancer cells often containsvariant forms as well as the full-length wild-typeform. In the present study, we investigated thesubcellular localization of HER2 protein in 1053primary breast cancer tissues. HER2 protein wasstained by various immunohistochemical methodsand studied by immunoelectron microscopy toconfirm the intracellular localization.Thirty-four of 1053 specimens showed cytoplasmicstaining of the intracellular domain of HER2protein by the HercepTest® and CB-11. In contrast,no immunoreactivity to the antibodiesagainst the extracellular domain was observed.None of the 34 specimens showed amplification ofthe HER2 protein by fluorescence in situ hybridization.Subsequently, we studied the associationof the cytoplasmic expression of HER2 with neuroendocrinedifferentiation. Interestingly, all 34specimens had some positive signals of neuroendocrinemarkers such as synaptophysin, chromogranin A, neuron-specific enolase, and CD56.Although the result is preliminary, it warrants furtherstudy on the role of the cytoplasmic variantform of HER2 in breast cancer growth, particularlyin the aspect of neuroendocrine differentiation.</description><subject>Adenocarcinoma, Mucinous - chemistry</subject><subject>Adenocarcinoma, Mucinous - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>breast cancer</subject><subject>Breast Neoplasms - chemistry</subject><subject>Breast Neoplasms - pathology</subject><subject>Carcinoma, Ductal, Breast - chemistry</subject><subject>Carcinoma, Ductal, Breast - pathology</subject><subject>Carcinoma, Lobular - chemistry</subject><subject>Carcinoma, Lobular - pathology</subject><subject>CD56 Antigen - analysis</subject><subject>Cell Differentiation</subject><subject>Chromogranin A - analysis</subject><subject>Coloring Agents</subject><subject>Cytoplasm - chemistry</subject><subject>cytoplasmic staining</subject><subject>Dentistry</subject><subject>Female</subject><subject>HER2/neu</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>immunohistochemistry</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Middle Aged</subject><subject>Neuroendocrine Cells - pathology</subject><subject>neuroendocrine differentiation</subject><subject>Phosphopyruvate Hydratase - analysis</subject><subject>Receptor, ErbB-2 - analysis</subject><subject>Receptor, ErbB-2 - metabolism</subject><subject>Synaptophysin - analysis</subject><subject>Young Adult</subject><issn>1342-8810</issn><issn>2185-9132</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo90MFKJDEQgOGwrKyDetvzkpun1lTSmWSOIq4uCILouUkqFc3QnR6TDOLbO8O4XqoO9VGHn7HfIC4Aeisu11OoF9oIKfQPtpBgdbcCJX-yBahedtaCOGZntSYvpAJtQK9-sWMJwqgdWLBwd_PYyctMW44fbd6Mrk4JeW0u5ZRfeKoc51JodI0Cf0_tle9smSmHGUvKxEOKkQrlllxLc-Ypc1_I1cbRFUx5ntwpO4purHT2tU_Y89-bp-u77v7h9t_11X23VmBa5yXEgAoCRkQPykTjo1MyCi-0i6vYR08SMABqi8slqKX1hHpF0AfZW3XCzg9_N2V-21Jtw5Qq0ji6TPO2DsYa0VtQe_nnS279RGHYlDS58jH8D7MDVwew3qV4oW_gSks40rDvPmgzyP049P--4asrA2X1CRv7f8k</recordid><startdate>201006</startdate><enddate>201006</enddate><creator>Horiguchi, Shin-ichiro</creator><creator>Hishima, Tsunekazu</creator><creator>Hayashi, Yukiko</creator><creator>Shiozawa, Yumiko</creator><creator>Horiguchi, Kazumi</creator><creator>Kuroi, Katsumasa</creator><creator>Toi, Masakazu</creator><creator>Funata, Nobuaki</creator><creator>Eishi, Yoshinobu</creator><general>Tokyo Medical and Dental University (TMDU)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201006</creationdate><title>HER-2/neu cytoplasmic staining is correlated with neuroendocrine differentiation in breast carcinoma</title><author>Horiguchi, Shin-ichiro ; Hishima, Tsunekazu ; Hayashi, Yukiko ; Shiozawa, Yumiko ; Horiguchi, Kazumi ; Kuroi, Katsumasa ; Toi, Masakazu ; Funata, Nobuaki ; Eishi, Yoshinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j317t-b21fdc31dcfccb137f7bfa32f0b05af9f4fbe21cd1c58c661368bec59e14d2483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adenocarcinoma, Mucinous - chemistry</topic><topic>Adenocarcinoma, Mucinous - pathology</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>breast cancer</topic><topic>Breast Neoplasms - chemistry</topic><topic>Breast Neoplasms - pathology</topic><topic>Carcinoma, Ductal, Breast - chemistry</topic><topic>Carcinoma, Ductal, Breast - pathology</topic><topic>Carcinoma, Lobular - chemistry</topic><topic>Carcinoma, Lobular - pathology</topic><topic>CD56 Antigen - analysis</topic><topic>Cell Differentiation</topic><topic>Chromogranin A - analysis</topic><topic>Coloring Agents</topic><topic>Cytoplasm - chemistry</topic><topic>cytoplasmic staining</topic><topic>Dentistry</topic><topic>Female</topic><topic>HER2/neu</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>immunohistochemistry</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Middle Aged</topic><topic>Neuroendocrine Cells - pathology</topic><topic>neuroendocrine differentiation</topic><topic>Phosphopyruvate Hydratase - analysis</topic><topic>Receptor, ErbB-2 - analysis</topic><topic>Receptor, ErbB-2 - metabolism</topic><topic>Synaptophysin - analysis</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horiguchi, Shin-ichiro</creatorcontrib><creatorcontrib>Hishima, Tsunekazu</creatorcontrib><creatorcontrib>Hayashi, Yukiko</creatorcontrib><creatorcontrib>Shiozawa, Yumiko</creatorcontrib><creatorcontrib>Horiguchi, Kazumi</creatorcontrib><creatorcontrib>Kuroi, Katsumasa</creatorcontrib><creatorcontrib>Toi, Masakazu</creatorcontrib><creatorcontrib>Funata, Nobuaki</creatorcontrib><creatorcontrib>Eishi, Yoshinobu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Medical and Dental Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horiguchi, Shin-ichiro</au><au>Hishima, Tsunekazu</au><au>Hayashi, Yukiko</au><au>Shiozawa, Yumiko</au><au>Horiguchi, Kazumi</au><au>Kuroi, Katsumasa</au><au>Toi, Masakazu</au><au>Funata, Nobuaki</au><au>Eishi, Yoshinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HER-2/neu cytoplasmic staining is correlated with neuroendocrine differentiation in breast carcinoma</atitle><jtitle>Journal of Medical and Dental Sciences</jtitle><addtitle>J. med. dent. sci.</addtitle><date>2010-06</date><risdate>2010</risdate><volume>57</volume><issue>2</issue><spage>155</spage><epage>163</epage><pages>155-163</pages><issn>1342-8810</issn><eissn>2185-9132</eissn><abstract>HER2 oncoprotein plays an essential role inbreast cancer growth and differentiation.Determination of HER2 status contributes not onlyto predicting survival but also to selecting thepatients for anti-HER2 therapy. HER2 proteinexpressed in human cancer cells often containsvariant forms as well as the full-length wild-typeform. In the present study, we investigated thesubcellular localization of HER2 protein in 1053primary breast cancer tissues. HER2 protein wasstained by various immunohistochemical methodsand studied by immunoelectron microscopy toconfirm the intracellular localization.Thirty-four of 1053 specimens showed cytoplasmicstaining of the intracellular domain of HER2protein by the HercepTest® and CB-11. In contrast,no immunoreactivity to the antibodiesagainst the extracellular domain was observed.None of the 34 specimens showed amplification ofthe HER2 protein by fluorescence in situ hybridization.Subsequently, we studied the associationof the cytoplasmic expression of HER2 with neuroendocrinedifferentiation. Interestingly, all 34specimens had some positive signals of neuroendocrinemarkers such as synaptophysin, chromogranin A, neuron-specific enolase, and CD56.Although the result is preliminary, it warrants furtherstudy on the role of the cytoplasmic variantform of HER2 in breast cancer growth, particularlyin the aspect of neuroendocrine differentiation.</abstract><cop>Japan</cop><pub>Tokyo Medical and Dental University (TMDU)</pub><pmid>21073134</pmid><doi>10.11480/jmds.570205</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1342-8810 |
| ispartof | Journal of Medical and Dental Sciences, 2010, Vol.57(2), pp.155-163 |
| issn | 1342-8810 2185-9132 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_787048138 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Alma/SFX Local Collection |
| subjects | Adenocarcinoma, Mucinous - chemistry Adenocarcinoma, Mucinous - pathology Adult Aged Aged, 80 and over breast cancer Breast Neoplasms - chemistry Breast Neoplasms - pathology Carcinoma, Ductal, Breast - chemistry Carcinoma, Ductal, Breast - pathology Carcinoma, Lobular - chemistry Carcinoma, Lobular - pathology CD56 Antigen - analysis Cell Differentiation Chromogranin A - analysis Coloring Agents Cytoplasm - chemistry cytoplasmic staining Dentistry Female HER2/neu Humans Immunoenzyme Techniques immunohistochemistry In Situ Hybridization, Fluorescence Middle Aged Neuroendocrine Cells - pathology neuroendocrine differentiation Phosphopyruvate Hydratase - analysis Receptor, ErbB-2 - analysis Receptor, ErbB-2 - metabolism Synaptophysin - analysis Young Adult |
| title | HER-2/neu cytoplasmic staining is correlated with neuroendocrine differentiation in breast carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T05%3A30%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=HER-2/neu%20cytoplasmic%20staining%20is%20correlated%20with%20neuroendocrine%20differentiation%20in%20breast%20carcinoma&rft.jtitle=Journal%20of%20Medical%20and%20Dental%20Sciences&rft.au=Horiguchi,%20Shin-ichiro&rft.date=2010-06&rft.volume=57&rft.issue=2&rft.spage=155&rft.epage=163&rft.pages=155-163&rft.issn=1342-8810&rft.eissn=2185-9132&rft_id=info:doi/10.11480/jmds.570205&rft_dat=%3Cproquest_pubme%3E787048138%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=787048138&rft_id=info:pmid/21073134&rfr_iscdi=true |