New method for spectrofluorometer monochromator wavelength calibration
A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristic absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fl...
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| Veröffentlicht in: | Journal of biochemical and biophysical methods 1988-09, Vol.17 (1), p.61-66 |
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| container_title | Journal of biochemical and biophysical methods |
| container_volume | 17 |
| creator | Paladini, A A Erijman, L |
| description | A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristic absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment. |
| doi_str_mv | 10.1016/0165-022X(88)90079-6 |
| format | Article |
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It is based on the distortion that the characteristic absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment.</description><identifier>ISSN: 0165-022X</identifier><identifier>DOI: 10.1016/0165-022X(88)90079-6</identifier><identifier>PMID: 3235768</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Calibration ; fluorescence ; spectrofluorometry ; Spectrometry, Fluorescence - instrumentation ; spectroscopy ; wavelength ; Weights and Measures</subject><ispartof>Journal of biochemical and biophysical methods, 1988-09, Vol.17 (1), p.61-66</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3235768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paladini, A A</creatorcontrib><creatorcontrib>Erijman, L</creatorcontrib><title>New method for spectrofluorometer monochromator wavelength calibration</title><title>Journal of biochemical and biophysical methods</title><addtitle>J Biochem Biophys Methods</addtitle><description>A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristic absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment.</description><subject>Calibration</subject><subject>fluorescence</subject><subject>spectrofluorometry</subject><subject>Spectrometry, Fluorescence - instrumentation</subject><subject>spectroscopy</subject><subject>wavelength</subject><subject>Weights and Measures</subject><issn>0165-022X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAURT2ASin8A5AyIRgCz3bijxFVFJAqWDqwRY7zQoKSONgOFf-eSFSsDE9X9-joDo-QCwq3FKi4my9PgbG3a6VuNIDUqTgiyz98Qk5D-AAArli2IAvOeC6FWpLNC-6THmPjqqR2Pgkj2uhd3U3Ou5mjT3o3ONvMzcRZ2Jsv7HB4j01iTdeW3sTWDWfkuDZdwPNDrshu87BbP6Xb18fn9f02HanQMaXcMpSZMLTUNEMtMwBEyfKyZgZFbSqQaABqpqSUduYVVoZpVVqQXPAVufqdHb37nDDEom-Dxa4zA7opFFIJrSHP_xVpTgFYzmbx8iBOZY9VMfq2N_67ODyI_wCvCWfa</recordid><startdate>19880901</startdate><enddate>19880901</enddate><creator>Paladini, A A</creator><creator>Erijman, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19880901</creationdate><title>New method for spectrofluorometer monochromator wavelength calibration</title><author>Paladini, A A ; Erijman, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p169t-13c2e746a1b914e97400ee725bf2ae6fad07ea00f28777c25bdeda298bc07363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Calibration</topic><topic>fluorescence</topic><topic>spectrofluorometry</topic><topic>Spectrometry, Fluorescence - instrumentation</topic><topic>spectroscopy</topic><topic>wavelength</topic><topic>Weights and Measures</topic><toplevel>online_resources</toplevel><creatorcontrib>Paladini, A A</creatorcontrib><creatorcontrib>Erijman, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemical and biophysical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paladini, A A</au><au>Erijman, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New method for spectrofluorometer monochromator wavelength calibration</atitle><jtitle>Journal of biochemical and biophysical methods</jtitle><addtitle>J Biochem Biophys Methods</addtitle><date>1988-09-01</date><risdate>1988</risdate><volume>17</volume><issue>1</issue><spage>61</spage><epage>66</epage><pages>61-66</pages><issn>0165-022X</issn><abstract>A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristic absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment.</abstract><cop>Netherlands</cop><pmid>3235768</pmid><doi>10.1016/0165-022X(88)90079-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-022X |
| ispartof | Journal of biochemical and biophysical methods, 1988-09, Vol.17 (1), p.61-66 |
| issn | 0165-022X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78699055 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Calibration fluorescence spectrofluorometry Spectrometry, Fluorescence - instrumentation spectroscopy wavelength Weights and Measures |
| title | New method for spectrofluorometer monochromator wavelength calibration |
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