Independent self-assembly of cadmium-binding α-fragment of metallothionein in Escherichia coli without participation of β-fragment

We examined the independent self-assembly of the α- and β-fragments of human metallothionein (MT) into cadmiumbinding conformation in an Escherichia coli expression system, in addition to wild-type MT expression. The expressed α-fragment formed independently the structure of a metal-binding cluster...

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