Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction

A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We compared the results obtained with the combined technique (HOS‐Spermac) with those obtained with currently...

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Veröffentlicht in:	Journal of andrology 1996-11, Vol.17 (6), p.692-698
Hauptverfasser:	Viggiano, J. M, Herrero, M. B, Martinez, S. P, De Gimeno, M. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosome - drug effects Acrosome - enzymology acrosome reaction Animals Arginine - pharmacology Biological and medical sciences Cell Size Cell Survival - physiology Enzyme Inhibitors - pharmacology Fluorescent Dyes Fundamental and applied biological sciences. Psychology Male Mammalian male genital system Mice Mice, Inbred C57BL Mice, Inbred CBA Morphology. Physiology NG-Nitroarginine Methyl Ester - pharmacology Nitric oxide synthase Nitric Oxide Synthase - antagonists & inhibitors Nitric Oxide Synthase - metabolism Spermatozoa - cytology Spermatozoa - enzymology Spermatozoa - ultrastructure Staining and Labeling - methods Time Factors Vertebrates: reproduction
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