Multiple viral determinants affect seed transmission of pea seedborne mosaic virus in Pisum sativum
1 Biotechnology Group, The Danish Institute of Plant and Soil Science, Lyngby, DK-2800, Denmark 2 Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804, USA 3 Department of Botany and Plant Pathology, Oregon State University, Corvall...
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| Veröffentlicht in: | Journal of general virology 1996-12, Vol.77 (12), p.3149-3154 |
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| container_issue | 12 |
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| container_title | Journal of general virology |
| container_volume | 77 |
| creator | Johansen, I. E Dougherty, W. G Keller, K. E Wang, D Hampton, R. O |
| description | 1 Biotechnology Group, The Danish Institute of Plant and Soil Science, Lyngby, DK-2800, Denmark
2 Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804, USA
3 Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902, USA
4 Department of Virus Research, John Innes Centre, Norwich NR4 7UH, UK
Two pea seedborne mosaic potyvirus (PSbMV) isolates, P-1 DPD1 (P-1), which is highly seed-transmitted, and P-4 NY (P-4), which is rarely seed-transmitted, and chimeras between P-1 and P-4 were analysed to map the viral genetic determinants of seed transmission. Infectivity of chimeric viruses was evaluated by inoculating Pisum sativum with RNA transcribed in vitro from recombinant full-length cDNA clones. The chimeric viruses that were used demonstrated that a genomic segment encoding the 49 kDa protease and putative RNA polymerase was responsible for symptom induction. Attempts to determine transmission of the chimeric viruses in P. sativum cultivars known to transmit P-1 at high frequencies showed that seed transmission is a quantitative character influenced by multiple viral determinants. Seed transmission frequency did not correlate with accumulation of virus in vegetative tissue. The 5' 2·5 kb of the 10 kb PSbMV genome had a major influence on the seed transmission frequency and was analysed further. This showed that, while the helper-component protease was a major determinant of seed transmission, the potyviral P1-protease exerted no measurable influence.
Received 13 June 1996;
accepted 22 August 1996. |
| doi_str_mv | 10.1099/0022-1317-77-12-3149 |
| format | Article |
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2 Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804, USA
3 Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902, USA
4 Department of Virus Research, John Innes Centre, Norwich NR4 7UH, UK
Two pea seedborne mosaic potyvirus (PSbMV) isolates, P-1 DPD1 (P-1), which is highly seed-transmitted, and P-4 NY (P-4), which is rarely seed-transmitted, and chimeras between P-1 and P-4 were analysed to map the viral genetic determinants of seed transmission. Infectivity of chimeric viruses was evaluated by inoculating Pisum sativum with RNA transcribed in vitro from recombinant full-length cDNA clones. The chimeric viruses that were used demonstrated that a genomic segment encoding the 49 kDa protease and putative RNA polymerase was responsible for symptom induction. Attempts to determine transmission of the chimeric viruses in P. sativum cultivars known to transmit P-1 at high frequencies showed that seed transmission is a quantitative character influenced by multiple viral determinants. Seed transmission frequency did not correlate with accumulation of virus in vegetative tissue. The 5' 2·5 kb of the 10 kb PSbMV genome had a major influence on the seed transmission frequency and was analysed further. This showed that, while the helper-component protease was a major determinant of seed transmission, the potyviral P1-protease exerted no measurable influence.
Received 13 June 1996;
accepted 22 August 1996.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-77-12-3149</identifier><identifier>PMID: 9000110</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Cloning, Molecular ; DNA, Complementary ; pea seed-borne mosaic virus ; Pisum sativum ; Pisum sativum - virology ; Potyvirus - genetics ; Potyvirus - isolation & purification ; Potyvirus - pathogenicity ; Recombination, Genetic ; RNA, Viral ; Seeds - virology</subject><ispartof>Journal of general virology, 1996-12, Vol.77 (12), p.3149-3154</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-6bc2a8d4ae0d6569e861a4a88e2f35d3d8ddd556ea2ee22707b1f4a06d063d093</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9000110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johansen, I. E</creatorcontrib><creatorcontrib>Dougherty, W. G</creatorcontrib><creatorcontrib>Keller, K. E</creatorcontrib><creatorcontrib>Wang, D</creatorcontrib><creatorcontrib>Hampton, R. O</creatorcontrib><title>Multiple viral determinants affect seed transmission of pea seedborne mosaic virus in Pisum sativum</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Biotechnology Group, The Danish Institute of Plant and Soil Science, Lyngby, DK-2800, Denmark
2 Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804, USA
3 Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902, USA
4 Department of Virus Research, John Innes Centre, Norwich NR4 7UH, UK
Two pea seedborne mosaic potyvirus (PSbMV) isolates, P-1 DPD1 (P-1), which is highly seed-transmitted, and P-4 NY (P-4), which is rarely seed-transmitted, and chimeras between P-1 and P-4 were analysed to map the viral genetic determinants of seed transmission. Infectivity of chimeric viruses was evaluated by inoculating Pisum sativum with RNA transcribed in vitro from recombinant full-length cDNA clones. The chimeric viruses that were used demonstrated that a genomic segment encoding the 49 kDa protease and putative RNA polymerase was responsible for symptom induction. Attempts to determine transmission of the chimeric viruses in P. sativum cultivars known to transmit P-1 at high frequencies showed that seed transmission is a quantitative character influenced by multiple viral determinants. Seed transmission frequency did not correlate with accumulation of virus in vegetative tissue. The 5' 2·5 kb of the 10 kb PSbMV genome had a major influence on the seed transmission frequency and was analysed further. This showed that, while the helper-component protease was a major determinant of seed transmission, the potyviral P1-protease exerted no measurable influence.
Received 13 June 1996;
accepted 22 August 1996.</description><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>pea seed-borne mosaic virus</subject><subject>Pisum sativum</subject><subject>Pisum sativum - virology</subject><subject>Potyvirus - genetics</subject><subject>Potyvirus - isolation & purification</subject><subject>Potyvirus - pathogenicity</subject><subject>Recombination, Genetic</subject><subject>RNA, Viral</subject><subject>Seeds - virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctOwzAQRS0EKqXwByB5hcQiYDt-JEtU8ZKKYAFry4knrVEexXaK-HsSWlXsWM1I98yd0R2Ezim5piTPbwhhLKEpVYlSCWVJSnl-gKaUS5GwAThE0z1yjE5C-CCEci7UBE1yMvSUTFH53NfRrWvAG-dNjS1E8I1rTRsDNlUFZcQBwOLoTRsaF4LrWtxVeA3mVyg63wJuumBcOXr0AbsWv7rQNziY6DZ9c4qOKlMHONvVGXq_v3ubPyaLl4en-e0iKTlNYyKLkpnMcgPESiFzyCQ13GQZsCoVNrWZtVYICYYBMKaIKmjFDZGWyNSSPJ2hy63v2nefPYSoh3tLqGvTQtcHrTKZCZHLf0EqMsGUSAeQb8HSdyF4qPTau8b4b02JHp-gx4T1mLBWSlOmxycMYxc7_75owO6HdqkP-tVWX7nl6st50EtoGzcsKVynhxD_eP0Axa2R-g</recordid><startdate>19961201</startdate><enddate>19961201</enddate><creator>Johansen, I. E</creator><creator>Dougherty, W. G</creator><creator>Keller, K. E</creator><creator>Wang, D</creator><creator>Hampton, R. O</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19961201</creationdate><title>Multiple viral determinants affect seed transmission of pea seedborne mosaic virus in Pisum sativum</title><author>Johansen, I. E ; Dougherty, W. G ; Keller, K. E ; Wang, D ; Hampton, R. O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-6bc2a8d4ae0d6569e861a4a88e2f35d3d8ddd556ea2ee22707b1f4a06d063d093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>pea seed-borne mosaic virus</topic><topic>Pisum sativum</topic><topic>Pisum sativum - virology</topic><topic>Potyvirus - genetics</topic><topic>Potyvirus - isolation & purification</topic><topic>Potyvirus - pathogenicity</topic><topic>Recombination, Genetic</topic><topic>RNA, Viral</topic><topic>Seeds - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johansen, I. E</creatorcontrib><creatorcontrib>Dougherty, W. G</creatorcontrib><creatorcontrib>Keller, K. E</creatorcontrib><creatorcontrib>Wang, D</creatorcontrib><creatorcontrib>Hampton, R. O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johansen, I. E</au><au>Dougherty, W. G</au><au>Keller, K. E</au><au>Wang, D</au><au>Hampton, R. O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple viral determinants affect seed transmission of pea seedborne mosaic virus in Pisum sativum</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1996-12-01</date><risdate>1996</risdate><volume>77</volume><issue>12</issue><spage>3149</spage><epage>3154</epage><pages>3149-3154</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>1 Biotechnology Group, The Danish Institute of Plant and Soil Science, Lyngby, DK-2800, Denmark
2 Department of Microbiology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis 97331-3804, USA
3 Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902, USA
4 Department of Virus Research, John Innes Centre, Norwich NR4 7UH, UK
Two pea seedborne mosaic potyvirus (PSbMV) isolates, P-1 DPD1 (P-1), which is highly seed-transmitted, and P-4 NY (P-4), which is rarely seed-transmitted, and chimeras between P-1 and P-4 were analysed to map the viral genetic determinants of seed transmission. Infectivity of chimeric viruses was evaluated by inoculating Pisum sativum with RNA transcribed in vitro from recombinant full-length cDNA clones. The chimeric viruses that were used demonstrated that a genomic segment encoding the 49 kDa protease and putative RNA polymerase was responsible for symptom induction. Attempts to determine transmission of the chimeric viruses in P. sativum cultivars known to transmit P-1 at high frequencies showed that seed transmission is a quantitative character influenced by multiple viral determinants. Seed transmission frequency did not correlate with accumulation of virus in vegetative tissue. The 5' 2·5 kb of the 10 kb PSbMV genome had a major influence on the seed transmission frequency and was analysed further. This showed that, while the helper-component protease was a major determinant of seed transmission, the potyviral P1-protease exerted no measurable influence.
Received 13 June 1996;
accepted 22 August 1996.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>9000110</pmid><doi>10.1099/0022-1317-77-12-3149</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1996-12, Vol.77 (12), p.3149-3154 |
| issn | 0022-1317 1465-2099 |
| language | eng |
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| source | MEDLINE; Microbiology Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Cloning, Molecular DNA, Complementary pea seed-borne mosaic virus Pisum sativum Pisum sativum - virology Potyvirus - genetics Potyvirus - isolation & purification Potyvirus - pathogenicity Recombination, Genetic RNA, Viral Seeds - virology |
| title | Multiple viral determinants affect seed transmission of pea seedborne mosaic virus in Pisum sativum |
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