Effects of Subtilisin Cleavage of Monomeric Actin on Its Nucleotide Binding

The kinetics of ATP exchange on subtilisin-cleaved G-actin was investigated by measuring the fluorescence of 1,N6-ethenoadenosine 5'-triphosphate. The apparent dissociation rate of ATP (k−ATP) was 2.8-fold larger than that of intact G-actin in the presence of 300 μM free Ca2+. Analysis of the d...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of biochemistry (Tokyo) 1996-12, Vol.120 (6), p.1104-1110
Hauptverfasser:	Ooi, Atsushi, Mihashi, Koshin
Format:	Artikel
Sprache:	eng
Schlagworte:	actin Actins - metabolism Adenosine Diphosphate - metabolism Adenosine Triphosphate - metabolism Animals Calcium - metabolism divalent cation DNA - metabolism Ethenoadenosine Triphosphate - metabolism Kinetics nucleotide Rabbits subtilisin Subtilisins - metabolism surface loop
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!