Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis
Phage G4 origin of complementary DNA strand synthesis ( ori c) consists of three stable stem-loop structures (I, II, and III). Mutant ori c sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II–III spacer region have been constructed and cloned into the fil...
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| Veröffentlicht in: | Gene 1988-11, Vol.71 (2), p.323-330 |
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| container_title | Gene |
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| creator | Hiroshi, Sakai Hiroshi, Hiasa Keiji, Iwamoto Taizo, Horimoto Tohru, Komano G. Nigel, Godson |
| description | Phage G4 origin of complementary DNA strand synthesis (
ori
c) consists of three stable stem-loop structures (I, II, and III). Mutant
ori
c sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II–III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo
ori
c function. The results suggest that recognition sequences for
dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III. |
| doi_str_mv | 10.1016/0378-1119(88)90049-2 |
| format | Article |
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ori
c) consists of three stable stem-loop structures (I, II, and III). Mutant
ori
c sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II–III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo
ori
c function. The results suggest that recognition sequences for
dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(88)90049-2</identifier><identifier>PMID: 3224827</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Bacteriophage genome replication ; Bacteriophages - genetics ; Biological and medical sciences ; Buffers ; cloned oric ; Culture Media ; DNA Replication ; dnaG primase ; Fundamental and applied biological sciences. Psychology ; Genetics ; M13 phage vector ; Microbiology ; mutants ; Mutation ; Nucleic Acid Conformation ; phage G4 ; recombinant DNA ; RNA primer ; stem-loop structures ; Virology</subject><ispartof>Gene, 1988-11, Vol.71 (2), p.323-330</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-5675c93515f5b301b905ccc36eda52d514e94a8997c8bd59a43e572fd0ab9d7f3</citedby><cites>FETCH-LOGICAL-c417t-5675c93515f5b301b905ccc36eda52d514e94a8997c8bd59a43e572fd0ab9d7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(88)90049-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7346676$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3224827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiroshi, Sakai</creatorcontrib><creatorcontrib>Hiroshi, Hiasa</creatorcontrib><creatorcontrib>Keiji, Iwamoto</creatorcontrib><creatorcontrib>Taizo, Horimoto</creatorcontrib><creatorcontrib>Tohru, Komano</creatorcontrib><creatorcontrib>G. Nigel, Godson</creatorcontrib><title>Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis</title><title>Gene</title><addtitle>Gene</addtitle><description>Phage G4 origin of complementary DNA strand synthesis (
ori
c) consists of three stable stem-loop structures (I, II, and III). Mutant
ori
c sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II–III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo
ori
c function. The results suggest that recognition sequences for
dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.</description><subject>Bacteriophage genome replication</subject><subject>Bacteriophages - genetics</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>cloned oric</subject><subject>Culture Media</subject><subject>DNA Replication</subject><subject>dnaG primase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>M13 phage vector</subject><subject>Microbiology</subject><subject>mutants</subject><subject>Mutation</subject><subject>Nucleic Acid Conformation</subject><subject>phage G4</subject><subject>recombinant DNA</subject><subject>RNA primer</subject><subject>stem-loop structures</subject><subject>Virology</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFTEQxYMo9bb6DRTyIEUftubvJnkRpFgVCkLR55BNZm8ju5s12RX67ZvtvdxHzcsQ5ncOM2cQekPJFSW0_Ui40g2l1LzX-oMhRJiGPUM7qpVpCOH6OdqdkJfovJTfpD4p2Rk644wJzdQO-bs0AE49Xu4Bz2mBaYluwAV8moLLD7gsefXLmqHgOOH53u0B7wVOOe7rvwp9GucBxirc8DC5TeKmgMvDVE1LLK_Qi94NBV4f6wX6dfPl5_W35vbH1-_Xn28bL6haGtkq6Q2XVPay44R2hkjvPW8hOMmCpAKMcNoY5XUXpHGCg1SsD8R1JqieX6DLg--c058VymLHWDwMg5sgrcUq3bbMqPa_IJWUEcNpBcUB9DmVkqG3c45j3dNSYrcj2C1huyVstbZPR7Csyt4e_dduhHASHVOv_XfHviveDX2Ny8dywhQXbfs05qcDBjW0vxGyLT7C5CHEDH6xIcV_z_EITlajhg</recordid><startdate>19881130</startdate><enddate>19881130</enddate><creator>Hiroshi, Sakai</creator><creator>Hiroshi, Hiasa</creator><creator>Keiji, Iwamoto</creator><creator>Taizo, Horimoto</creator><creator>Tohru, Komano</creator><creator>G. 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Nigel, Godson</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-5675c93515f5b301b905ccc36eda52d514e94a8997c8bd59a43e572fd0ab9d7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Bacteriophage genome replication</topic><topic>Bacteriophages - genetics</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>cloned oric</topic><topic>Culture Media</topic><topic>DNA Replication</topic><topic>dnaG primase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>M13 phage vector</topic><topic>Microbiology</topic><topic>mutants</topic><topic>Mutation</topic><topic>Nucleic Acid Conformation</topic><topic>phage G4</topic><topic>recombinant DNA</topic><topic>RNA primer</topic><topic>stem-loop structures</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiroshi, Sakai</creatorcontrib><creatorcontrib>Hiroshi, Hiasa</creatorcontrib><creatorcontrib>Keiji, Iwamoto</creatorcontrib><creatorcontrib>Taizo, Horimoto</creatorcontrib><creatorcontrib>Tohru, Komano</creatorcontrib><creatorcontrib>G. 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ori
c) consists of three stable stem-loop structures (I, II, and III). Mutant
ori
c sequences with alterations in the structure of stem-loop II, stem-loop III, and the stem-loop II–III spacer region have been constructed and cloned into the filamentous phage vectors to assay their functional activity. Changes in the lowermost GC base pair in the stem of stem-loop III, in the 9-bp spacer region between the stems of stem-loops II and III, and in the loop of stem-loop II, impair or abolish in vivo
ori
c function. The results suggest that recognition sequences for
dnaG primase must be present in the loop of stem-loop II, and in the spacer region between the stems of stem-loops II and III.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3224827</pmid><doi>10.1016/0378-1119(88)90049-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | Gene, 1988-11, Vol.71 (2), p.323-330 |
| issn | 0378-1119 1879-0038 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Bacteriophage genome replication Bacteriophages - genetics Biological and medical sciences Buffers cloned oric Culture Media DNA Replication dnaG primase Fundamental and applied biological sciences. Psychology Genetics M13 phage vector Microbiology mutants Mutation Nucleic Acid Conformation phage G4 recombinant DNA RNA primer stem-loop structures Virology |
| title | Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis |
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