Cell lipids of the Candida lipolytica yeast grown on methanol
Candida lipolytica yeast, grown on 1% methanol as the only carbon and energy source, synthesized 4.9% of dry cell mass as lipids, 52.3% of which were polar lipids. Polar lipids consisted mainly of phospholipids and sphingolipids as their minor components. The total long-chain bases content has been...
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| Veröffentlicht in: | Journal of Chromatography A 1996-11, Vol.755 (1), p.75-80 |
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| container_title | Journal of Chromatography A |
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| creator | RUPCIC, J BLAGOVIC, B MARIC, V |
| description | Candida lipolytica yeast, grown on 1% methanol as the only carbon and energy source, synthesized 4.9% of dry cell mass as lipids, 52.3% of which were polar lipids. Polar lipids consisted mainly of phospholipids and sphingolipids as their minor components. The total long-chain bases content has been found to account only for 0.7% of the polar lipids. The long-chain bases composition determined by thin-layer and gas chromatography shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was the high content of 19-phytosphingosine (90.8% of total long-chain bases). Fatty acid (FA) composition of polar lipids was characterized by the relatively high concentration of unsaturated fatty acids (66.4% of total FA) and by the predominance of fatty acids with 16 carbon atoms (85.0% of total FA). |
| doi_str_mv | 10.1016/S0021-9673(96)00579-1 |
| format | Article |
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Polar lipids consisted mainly of phospholipids and sphingolipids as their minor components. The total long-chain bases content has been found to account only for 0.7% of the polar lipids. The long-chain bases composition determined by thin-layer and gas chromatography shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was the high content of 19-phytosphingosine (90.8% of total long-chain bases). Fatty acid (FA) composition of polar lipids was characterized by the relatively high concentration of unsaturated fatty acids (66.4% of total FA) and by the predominance of fatty acids with 16 carbon atoms (85.0% of total FA).</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(96)00579-1</identifier><identifier>PMID: 8997745</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Candida - chemistry ; Candida - growth & development ; Candida lipolytica ; Chromatography, Gas ; Chromatography, Thin Layer ; Fatty acids ; Fatty Acids - analysis ; Fundamental and applied biological sciences. Psychology ; Lipids ; Lipids - analysis ; Methanol ; Methods. Procedures. Technologies ; Phytosphingosines ; Sphingolipids ; Yeast</subject><ispartof>Journal of Chromatography A, 1996-11, Vol.755 (1), p.75-80</ispartof><rights>1996</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-b17df22be4df4bd5aaa875f90bc8e830e0fbf9a4563fa766f4140764c8d41b063</citedby><cites>FETCH-LOGICAL-c389t-b17df22be4df4bd5aaa875f90bc8e830e0fbf9a4563fa766f4140764c8d41b063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0021-9673(96)00579-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2516663$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8997745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RUPCIC, J</creatorcontrib><creatorcontrib>BLAGOVIC, B</creatorcontrib><creatorcontrib>MARIC, V</creatorcontrib><title>Cell lipids of the Candida lipolytica yeast grown on methanol</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>Candida lipolytica yeast, grown on 1% methanol as the only carbon and energy source, synthesized 4.9% of dry cell mass as lipids, 52.3% of which were polar lipids. Polar lipids consisted mainly of phospholipids and sphingolipids as their minor components. The total long-chain bases content has been found to account only for 0.7% of the polar lipids. The long-chain bases composition determined by thin-layer and gas chromatography shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was the high content of 19-phytosphingosine (90.8% of total long-chain bases). Fatty acid (FA) composition of polar lipids was characterized by the relatively high concentration of unsaturated fatty acids (66.4% of total FA) and by the predominance of fatty acids with 16 carbon atoms (85.0% of total FA).</description><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Candida - chemistry</subject><subject>Candida - growth & development</subject><subject>Candida lipolytica</subject><subject>Chromatography, Gas</subject><subject>Chromatography, Thin Layer</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Methanol</subject><subject>Methods. Procedures. Technologies</subject><subject>Phytosphingosines</subject><subject>Sphingolipids</subject><subject>Yeast</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAQgD2AChR-QqUMCMEQsJPYjgeEUMRLqsQAzJZjn6lREhc7BfXfk7ZRV5Y76e67hz6EZgRfE0zYzRvGGUkF4_mlYFcYUy5ScoCO9-UjdBLjF8aEY55N0KQUgvOCHqPbCpomadzSmZh4m_QLSCrVGWfUpuqbde-0StagYp98Bv_bJb5LWugXqvPNKTq0qolwNuYp-nh8eK-e0_nr00t1P091Xoo-rQk3NstqKIwtakOVUiWnVuBal1DmGLCtrVAFZblVnDFbkAJzVujSFKTGLJ-ii93eZfDfK4i9bF3Uw-eqA7-KkpeMloKIAaQ7UAcfYwArl8G1KqwlwXKjSm5VyY2TIcitKkmGudl4YFW3YPZTo6ehfz72VdSqsUF12sU9llHCGMsH7G6HwSDjx0GQUTvoNBgXQPfSePfPI39f24cU</recordid><startdate>19961129</startdate><enddate>19961129</enddate><creator>RUPCIC, J</creator><creator>BLAGOVIC, B</creator><creator>MARIC, V</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961129</creationdate><title>Cell lipids of the Candida lipolytica yeast grown on methanol</title><author>RUPCIC, J ; BLAGOVIC, B ; MARIC, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-b17df22be4df4bd5aaa875f90bc8e830e0fbf9a4563fa766f4140764c8d41b063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Candida - chemistry</topic><topic>Candida - growth & development</topic><topic>Candida lipolytica</topic><topic>Chromatography, Gas</topic><topic>Chromatography, Thin Layer</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lipids</topic><topic>Lipids - analysis</topic><topic>Methanol</topic><topic>Methods. Procedures. Technologies</topic><topic>Phytosphingosines</topic><topic>Sphingolipids</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RUPCIC, J</creatorcontrib><creatorcontrib>BLAGOVIC, B</creatorcontrib><creatorcontrib>MARIC, V</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RUPCIC, J</au><au>BLAGOVIC, B</au><au>MARIC, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell lipids of the Candida lipolytica yeast grown on methanol</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1996-11-29</date><risdate>1996</risdate><volume>755</volume><issue>1</issue><spage>75</spage><epage>80</epage><pages>75-80</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>Candida lipolytica yeast, grown on 1% methanol as the only carbon and energy source, synthesized 4.9% of dry cell mass as lipids, 52.3% of which were polar lipids. Polar lipids consisted mainly of phospholipids and sphingolipids as their minor components. The total long-chain bases content has been found to account only for 0.7% of the polar lipids. The long-chain bases composition determined by thin-layer and gas chromatography shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was the high content of 19-phytosphingosine (90.8% of total long-chain bases). Fatty acid (FA) composition of polar lipids was characterized by the relatively high concentration of unsaturated fatty acids (66.4% of total FA) and by the predominance of fatty acids with 16 carbon atoms (85.0% of total FA).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8997745</pmid><doi>10.1016/S0021-9673(96)00579-1</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Candida - chemistry Candida - growth & development Candida lipolytica Chromatography, Gas Chromatography, Thin Layer Fatty acids Fatty Acids - analysis Fundamental and applied biological sciences. Psychology Lipids Lipids - analysis Methanol Methods. Procedures. Technologies Phytosphingosines Sphingolipids Yeast |
| title | Cell lipids of the Candida lipolytica yeast grown on methanol |
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