Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus
A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support, has been developed for the detection and identification of virus in clinical samples. Reagents were standardized and, using purified Newcastle disease virus (NDV...
Gespeichert in:
| Veröffentlicht in: | Journal of virological methods 1988-12, Vol.22 (2), p.149-164 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 164 |
|---|---|
| container_issue | 2 |
| container_start_page | 149 |
| container_title | Journal of virological methods |
| container_volume | 22 |
| creator | Fulton, Roberta E. Wong, Jonathan P. Siddiqui, Yunus M. Tso, May-S. |
| description | A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support, has been developed for the detection and identification of virus in clinical samples. Reagents were standardized and, using purified Newcastle disease virus (NDV) as a model, the theoretical lower limits of test sensitivity of the FELISA were compared, in both “sandwich” and “indirect” formats, to those of a comparable chromogenic enzyme-linked immunosorbent assay (CELISA). Of the systems evaluated, the “sandwich” FELISA exhibited maximum sensitivity and detected 10 fg of purified virus protein per milliliter of test sample (500 ag per test volume). Specificity of the “sandwich” FELISA was evaluated by challenging the system with heterologous strains of NDV and with other serologically related and unrelated viruses. In a clinical trial in which fecal materials from chickens undergoing vaccination with NDV were assayed directly by FELISA, the virus was detected from the first to approximately the tenth day post-vaccination. The test is simple to perform and results can be obtained in approximately 4 h. |
| doi_str_mv | 10.1016/0166-0934(88)90098-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78658806</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0166093488900985</els_id><sourcerecordid>78658806</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-67fd830bd9630db65c0c3c73b9ddb817c6892be8c0db2af5091f45b5243a6d313</originalsourceid><addsrcrecordid>eNqFkEFrFTEQx0OptK-136BCDkX0sJpsNtnkIkixKhQ8qFdDNpmUyG7SJrsPnp_erO_xjvUQBjK_Gf7zQ-iakneUUPG-PtEQxbo3Ur5VhCjZ8BO0obJX9Vt2p2hzRM7RRSm_CSG8Z-wMnTHaCS7bDfr1HWIJc9gC9uOScnqAGCyG-Gc3AQ7TtMRkSjE7nCKOYc7JwjguYyqAJ5iGbCIU7FPGT4uJc5jNHCqZPN6GvJSX6IU3Y4GrQ71EP-8-_bj90tx_-_z19uN9Yzvaz43ovZOMDE4JRtwguCWW2Z4NyrlB0t4KqdoBpK3N1nhOFPUdH3jbMSMco-wSvd7vfczpaYEy6ymUNWmNl5aie1nPlUT8F6ScCiKVrGC3B21OpWTw-jGHyeSdpkSv_vUqV69ytZT6n3_N69irw_5lmMAdhw7Ca__m0DfFmtFXfzaUI9a39Sa-xvywx6BK2wbIutgA0YILGeysXQrP5_gLYOKjRg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15160898</pqid></control><display><type>article</type><title>Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Fulton, Roberta E. ; Wong, Jonathan P. ; Siddiqui, Yunus M. ; Tso, May-S.</creator><creatorcontrib>Fulton, Roberta E. ; Wong, Jonathan P. ; Siddiqui, Yunus M. ; Tso, May-S.</creatorcontrib><description>A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support, has been developed for the detection and identification of virus in clinical samples. Reagents were standardized and, using purified Newcastle disease virus (NDV) as a model, the theoretical lower limits of test sensitivity of the FELISA were compared, in both “sandwich” and “indirect” formats, to those of a comparable chromogenic enzyme-linked immunosorbent assay (CELISA). Of the systems evaluated, the “sandwich” FELISA exhibited maximum sensitivity and detected 10 fg of purified virus protein per milliliter of test sample (500 ag per test volume). Specificity of the “sandwich” FELISA was evaluated by challenging the system with heterologous strains of NDV and with other serologically related and unrelated viruses. In a clinical trial in which fecal materials from chickens undergoing vaccination with NDV were assayed directly by FELISA, the virus was detected from the first to approximately the tenth day post-vaccination. The test is simple to perform and results can be obtained in approximately 4 h.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(88)90098-5</identifier><identifier>PMID: 3146582</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Antigens, Viral - isolation & purification ; Biological and medical sciences ; Chickens ; Chromogenic Compounds ; Collodion ; Detection and identification ; Diagnosis ; Enzyme-Linked Immunosorbent Assay ; Feces - immunology ; Feces - microbiology ; Fluorescence immunoassay ; Fluorescent Dyes ; Fluorogenic enzyme-linked immunosorbent assay ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Newcastle disease virus ; Newcastle disease virus - immunology ; Newcastle disease virus - isolation & purification ; Nitrocellulose ; Techniques used in virology ; Viral Vaccines - immunology ; Virology ; Viruses - isolation & purification</subject><ispartof>Journal of virological methods, 1988-12, Vol.22 (2), p.149-164</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-67fd830bd9630db65c0c3c73b9ddb817c6892be8c0db2af5091f45b5243a6d313</citedby><cites>FETCH-LOGICAL-c417t-67fd830bd9630db65c0c3c73b9ddb817c6892be8c0db2af5091f45b5243a6d313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-0934(88)90098-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7252456$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3146582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fulton, Roberta E.</creatorcontrib><creatorcontrib>Wong, Jonathan P.</creatorcontrib><creatorcontrib>Siddiqui, Yunus M.</creatorcontrib><creatorcontrib>Tso, May-S.</creatorcontrib><title>Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support, has been developed for the detection and identification of virus in clinical samples. Reagents were standardized and, using purified Newcastle disease virus (NDV) as a model, the theoretical lower limits of test sensitivity of the FELISA were compared, in both “sandwich” and “indirect” formats, to those of a comparable chromogenic enzyme-linked immunosorbent assay (CELISA). Of the systems evaluated, the “sandwich” FELISA exhibited maximum sensitivity and detected 10 fg of purified virus protein per milliliter of test sample (500 ag per test volume). Specificity of the “sandwich” FELISA was evaluated by challenging the system with heterologous strains of NDV and with other serologically related and unrelated viruses. In a clinical trial in which fecal materials from chickens undergoing vaccination with NDV were assayed directly by FELISA, the virus was detected from the first to approximately the tenth day post-vaccination. The test is simple to perform and results can be obtained in approximately 4 h.</description><subject>Animals</subject><subject>Antigens, Viral - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Chickens</subject><subject>Chromogenic Compounds</subject><subject>Collodion</subject><subject>Detection and identification</subject><subject>Diagnosis</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Feces - immunology</subject><subject>Feces - microbiology</subject><subject>Fluorescence immunoassay</subject><subject>Fluorescent Dyes</subject><subject>Fluorogenic enzyme-linked immunosorbent assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Newcastle disease virus</subject><subject>Newcastle disease virus - immunology</subject><subject>Newcastle disease virus - isolation & purification</subject><subject>Nitrocellulose</subject><subject>Techniques used in virology</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><subject>Viruses - isolation & purification</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFrFTEQx0OptK-136BCDkX0sJpsNtnkIkixKhQ8qFdDNpmUyG7SJrsPnp_erO_xjvUQBjK_Gf7zQ-iakneUUPG-PtEQxbo3Ur5VhCjZ8BO0obJX9Vt2p2hzRM7RRSm_CSG8Z-wMnTHaCS7bDfr1HWIJc9gC9uOScnqAGCyG-Gc3AQ7TtMRkSjE7nCKOYc7JwjguYyqAJ5iGbCIU7FPGT4uJc5jNHCqZPN6GvJSX6IU3Y4GrQ71EP-8-_bj90tx_-_z19uN9Yzvaz43ovZOMDE4JRtwguCWW2Z4NyrlB0t4KqdoBpK3N1nhOFPUdH3jbMSMco-wSvd7vfczpaYEy6ymUNWmNl5aie1nPlUT8F6ScCiKVrGC3B21OpWTw-jGHyeSdpkSv_vUqV69ytZT6n3_N69irw_5lmMAdhw7Ca__m0DfFmtFXfzaUI9a39Sa-xvywx6BK2wbIutgA0YILGeysXQrP5_gLYOKjRg</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Fulton, Roberta E.</creator><creator>Wong, Jonathan P.</creator><creator>Siddiqui, Yunus M.</creator><creator>Tso, May-S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19881201</creationdate><title>Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus</title><author>Fulton, Roberta E. ; Wong, Jonathan P. ; Siddiqui, Yunus M. ; Tso, May-S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-67fd830bd9630db65c0c3c73b9ddb817c6892be8c0db2af5091f45b5243a6d313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Antigens, Viral - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Chickens</topic><topic>Chromogenic Compounds</topic><topic>Collodion</topic><topic>Detection and identification</topic><topic>Diagnosis</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Feces - immunology</topic><topic>Feces - microbiology</topic><topic>Fluorescence immunoassay</topic><topic>Fluorescent Dyes</topic><topic>Fluorogenic enzyme-linked immunosorbent assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Newcastle disease virus</topic><topic>Newcastle disease virus - immunology</topic><topic>Newcastle disease virus - isolation & purification</topic><topic>Nitrocellulose</topic><topic>Techniques used in virology</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><topic>Viruses - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fulton, Roberta E.</creatorcontrib><creatorcontrib>Wong, Jonathan P.</creatorcontrib><creatorcontrib>Siddiqui, Yunus M.</creatorcontrib><creatorcontrib>Tso, May-S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fulton, Roberta E.</au><au>Wong, Jonathan P.</au><au>Siddiqui, Yunus M.</au><au>Tso, May-S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>22</volume><issue>2</issue><spage>149</spage><epage>164</epage><pages>149-164</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support, has been developed for the detection and identification of virus in clinical samples. Reagents were standardized and, using purified Newcastle disease virus (NDV) as a model, the theoretical lower limits of test sensitivity of the FELISA were compared, in both “sandwich” and “indirect” formats, to those of a comparable chromogenic enzyme-linked immunosorbent assay (CELISA). Of the systems evaluated, the “sandwich” FELISA exhibited maximum sensitivity and detected 10 fg of purified virus protein per milliliter of test sample (500 ag per test volume). Specificity of the “sandwich” FELISA was evaluated by challenging the system with heterologous strains of NDV and with other serologically related and unrelated viruses. In a clinical trial in which fecal materials from chickens undergoing vaccination with NDV were assayed directly by FELISA, the virus was detected from the first to approximately the tenth day post-vaccination. The test is simple to perform and results can be obtained in approximately 4 h.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3146582</pmid><doi>10.1016/0166-0934(88)90098-5</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1988-12, Vol.22 (2), p.149-164 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78658806 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antigens, Viral - isolation & purification Biological and medical sciences Chickens Chromogenic Compounds Collodion Detection and identification Diagnosis Enzyme-Linked Immunosorbent Assay Feces - immunology Feces - microbiology Fluorescence immunoassay Fluorescent Dyes Fluorogenic enzyme-linked immunosorbent assay Fundamental and applied biological sciences. Psychology Microbiology Newcastle disease virus Newcastle disease virus - immunology Newcastle disease virus - isolation & purification Nitrocellulose Techniques used in virology Viral Vaccines - immunology Virology Viruses - isolation & purification |
| title | Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T05%3A10%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensitive%20fluorogenic%20enzyme%20immunoassay%20on%20nitrocellulose%20membranes%20for%20quantitation%20of%20virus&rft.jtitle=Journal%20of%20virological%20methods&rft.au=Fulton,%20Roberta%20E.&rft.date=1988-12-01&rft.volume=22&rft.issue=2&rft.spage=149&rft.epage=164&rft.pages=149-164&rft.issn=0166-0934&rft.eissn=1879-0984&rft.coden=JVMEDH&rft_id=info:doi/10.1016/0166-0934(88)90098-5&rft_dat=%3Cproquest_cross%3E78658806%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15160898&rft_id=info:pmid/3146582&rft_els_id=0166093488900985&rfr_iscdi=true |