Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube
OBJECTIVE: The fallopian tube is the site of fertilization and early embryonic growth and a common site of ectopic implantation. Although the factors responsible for early embryogenesis and implantation are incompletely understood, leukemia inhibitory factor may have an important role in early embry...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1996-12, Vol.175 (6), p.1611-1619 |
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| container_title | American journal of obstetrics and gynecology |
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| creator | Keltz, Martin D. Attar, Erkut Buradagunta, Sumati Olive, David L. Kliman, Harvey J. Arici, Aydin |
| description | OBJECTIVE: The fallopian tube is the site of fertilization and early embryonic growth and a common site of ectopic implantation. Although the factors responsible for early embryogenesis and implantation are incompletely understood, leukemia inhibitory factor may have an important role in early embryonic development and implantation. We set out to evaluate the production and modulation of leukemia inhibitory factor in the fallopian tube.
STUDY DESIGN: We first investigated leukemia inhibitory factor messenger ribonucleic acid levels in fallopian tubes. We then investigated leukemia inhibitory factor messenger ribonucleic acid and protein production in tubal epithelial and stromal cell cultures.
RESULTS: Leukemia inhibitory factor messenger ribonucleic acid is expressed in the fallopian tube with only slight variation during the menstrual cycle; however, it is markedly elevated in association with ectopic pregnancy. The level is higher in the tubal mucosa than in the remaining layers and is higher in the more distal segments of the fallopian tube. Estradiol and progesterone did not modulate leukemia inhibitory factor expression in epithelial or stromal cell cultures. Interleukin-1α, tumor necrosis factor-α, and transforming growth factor-β enhanced leukemia inhibitory factor expression in epithelial and stromal cells, with transforming growth factor-β1 enhancing expression by fourfold in stromal cells. Epithelial cells secreted high levels of leukemia inhibitory factor compared with stromal cells (332 ± 89 vs 25 ± 42 pg/mg total protein). Yet stromal cells treated with transforming growth factor-β alone or in combination with epidermal growth factor and platelet-derived growth factor, as well as TNF-α alone or in combination with interleukin-1α enhanced secretion of leukemia inhibitory factor at or above the levels found with epithelial cells.
CONCLUSIONS: We speculate that the high constitutive levels of leukemia inhibitory factor expressed in the ampullary portion of the fallopian tube may play a role in early embryonic development. Additionally, elevated expression with ectopic implantation and the marked induction of secretion in the tubal stroma by growth factors and cytokines suggest a link between inflammation, leukemia inhibitory factor, and tubal ectopic pregnancies. (Am J Obstet Gynecol 1996;175:1611-9.) |
| doi_str_mv | 10.1016/S0002-9378(96)70114-X |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78656571</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S000293789670114X</els_id><sourcerecordid>78656571</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-e3642bcdb0b0f32c7f9feafb831499fb86d4e56f4c12a0956e202af63f93c4613</originalsourceid><addsrcrecordid>eNqFkMFO3DAQhq0KBFvaR0DyASF6SLEdx4lPCCFakEAcaCVuluOMu4bEXuwEsW-Pw6722tOMPd9vjz6Ejin5SQkV54-EEFbIsm7OpPhRE0p58fQFLSiRdSEa0eyhxQ45RF9Tep6PTLIDdNDIppZcLtBwH7qp16MLHgeLe5heYHAaO790rRtDXGOrTa74H3jA8L6KkNJMa9_hVQwjOI9bF9Laj0tILuUozh1eToP2Odz3YeVyN04tfEP7-SLB9209Qn9_Xf-5uinuHn7fXl3eFYaXYiygFJy1pmtJS2zJTG2lBW3bpqRcylxFx6ESlhvKNJGVAEaYtqK0sjRc0PIInW7ezQu-TpBGNbhkoO-1hzAlVTeiElU9g9UGNDGkFMGqVXSDjmtFiZo1q0_NanaopFCfmtVTzh1vP5jaAbpdaus1z0-2c52M7m3U3ri0w1jFOaEzdrHBIMt4cxBVMg68gc5FMKPqgvvPIh9WF5xN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78656571</pqid></control><display><type>article</type><title>Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Keltz, Martin D. ; Attar, Erkut ; Buradagunta, Sumati ; Olive, David L. ; Kliman, Harvey J. ; Arici, Aydin</creator><creatorcontrib>Keltz, Martin D. ; Attar, Erkut ; Buradagunta, Sumati ; Olive, David L. ; Kliman, Harvey J. ; Arici, Aydin</creatorcontrib><description>OBJECTIVE: The fallopian tube is the site of fertilization and early embryonic growth and a common site of ectopic implantation. Although the factors responsible for early embryogenesis and implantation are incompletely understood, leukemia inhibitory factor may have an important role in early embryonic development and implantation. We set out to evaluate the production and modulation of leukemia inhibitory factor in the fallopian tube.
STUDY DESIGN: We first investigated leukemia inhibitory factor messenger ribonucleic acid levels in fallopian tubes. We then investigated leukemia inhibitory factor messenger ribonucleic acid and protein production in tubal epithelial and stromal cell cultures.
RESULTS: Leukemia inhibitory factor messenger ribonucleic acid is expressed in the fallopian tube with only slight variation during the menstrual cycle; however, it is markedly elevated in association with ectopic pregnancy. The level is higher in the tubal mucosa than in the remaining layers and is higher in the more distal segments of the fallopian tube. Estradiol and progesterone did not modulate leukemia inhibitory factor expression in epithelial or stromal cell cultures. Interleukin-1α, tumor necrosis factor-α, and transforming growth factor-β enhanced leukemia inhibitory factor expression in epithelial and stromal cells, with transforming growth factor-β1 enhancing expression by fourfold in stromal cells. Epithelial cells secreted high levels of leukemia inhibitory factor compared with stromal cells (332 ± 89 vs 25 ± 42 pg/mg total protein). Yet stromal cells treated with transforming growth factor-β alone or in combination with epidermal growth factor and platelet-derived growth factor, as well as TNF-α alone or in combination with interleukin-1α enhanced secretion of leukemia inhibitory factor at or above the levels found with epithelial cells.
CONCLUSIONS: We speculate that the high constitutive levels of leukemia inhibitory factor expressed in the ampullary portion of the fallopian tube may play a role in early embryonic development. Additionally, elevated expression with ectopic implantation and the marked induction of secretion in the tubal stroma by growth factors and cytokines suggest a link between inflammation, leukemia inhibitory factor, and tubal ectopic pregnancies. (Am J Obstet Gynecol 1996;175:1611-9.)</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/S0002-9378(96)70114-X</identifier><identifier>PMID: 8987949</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Mosby, Inc</publisher><subject>Adult ; Biological and medical sciences ; Blood Physiological Phenomena ; Cells, Cultured ; Culture Media ; Cytokines - pharmacology ; Diseases of mother, fetus and pregnancy ; ectopic pregnancy ; Epithelium - metabolism ; fallopian tube ; Fallopian Tubes - cytology ; Fallopian Tubes - metabolism ; Fallopian Tubes - physiology ; Female ; Gene Expression Regulation ; Growth Inhibitors - genetics ; Growth Inhibitors - metabolism ; Growth Substances - pharmacology ; Gynecology. Andrology. Obstetrics ; Hormones - pharmacology ; Humans ; Interleukin-6 ; Leukemia Inhibitory Factor ; Lymphokines - genetics ; Lymphokines - metabolism ; Medical sciences ; Middle Aged ; Pregnancy ; Pregnancy, Ectopic - metabolism ; Pregnancy. Fetus. Placenta ; RNA, Messenger - metabolism ; Stromal Cells - metabolism</subject><ispartof>American journal of obstetrics and gynecology, 1996-12, Vol.175 (6), p.1611-1619</ispartof><rights>1996 Mosby, Inc.</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-e3642bcdb0b0f32c7f9feafb831499fb86d4e56f4c12a0956e202af63f93c4613</citedby><cites>FETCH-LOGICAL-c436t-e3642bcdb0b0f32c7f9feafb831499fb86d4e56f4c12a0956e202af63f93c4613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0002-9378(96)70114-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>310,311,315,781,785,790,791,3551,23934,23935,25144,27928,27929,45999</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2544019$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8987949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Keltz, Martin D.</creatorcontrib><creatorcontrib>Attar, Erkut</creatorcontrib><creatorcontrib>Buradagunta, Sumati</creatorcontrib><creatorcontrib>Olive, David L.</creatorcontrib><creatorcontrib>Kliman, Harvey J.</creatorcontrib><creatorcontrib>Arici, Aydin</creatorcontrib><title>Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>OBJECTIVE: The fallopian tube is the site of fertilization and early embryonic growth and a common site of ectopic implantation. Although the factors responsible for early embryogenesis and implantation are incompletely understood, leukemia inhibitory factor may have an important role in early embryonic development and implantation. We set out to evaluate the production and modulation of leukemia inhibitory factor in the fallopian tube.
STUDY DESIGN: We first investigated leukemia inhibitory factor messenger ribonucleic acid levels in fallopian tubes. We then investigated leukemia inhibitory factor messenger ribonucleic acid and protein production in tubal epithelial and stromal cell cultures.
RESULTS: Leukemia inhibitory factor messenger ribonucleic acid is expressed in the fallopian tube with only slight variation during the menstrual cycle; however, it is markedly elevated in association with ectopic pregnancy. The level is higher in the tubal mucosa than in the remaining layers and is higher in the more distal segments of the fallopian tube. Estradiol and progesterone did not modulate leukemia inhibitory factor expression in epithelial or stromal cell cultures. Interleukin-1α, tumor necrosis factor-α, and transforming growth factor-β enhanced leukemia inhibitory factor expression in epithelial and stromal cells, with transforming growth factor-β1 enhancing expression by fourfold in stromal cells. Epithelial cells secreted high levels of leukemia inhibitory factor compared with stromal cells (332 ± 89 vs 25 ± 42 pg/mg total protein). Yet stromal cells treated with transforming growth factor-β alone or in combination with epidermal growth factor and platelet-derived growth factor, as well as TNF-α alone or in combination with interleukin-1α enhanced secretion of leukemia inhibitory factor at or above the levels found with epithelial cells.
CONCLUSIONS: We speculate that the high constitutive levels of leukemia inhibitory factor expressed in the ampullary portion of the fallopian tube may play a role in early embryonic development. Additionally, elevated expression with ectopic implantation and the marked induction of secretion in the tubal stroma by growth factors and cytokines suggest a link between inflammation, leukemia inhibitory factor, and tubal ectopic pregnancies. (Am J Obstet Gynecol 1996;175:1611-9.)</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Blood Physiological Phenomena</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Cytokines - pharmacology</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>ectopic pregnancy</subject><subject>Epithelium - metabolism</subject><subject>fallopian tube</subject><subject>Fallopian Tubes - cytology</subject><subject>Fallopian Tubes - metabolism</subject><subject>Fallopian Tubes - physiology</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Growth Inhibitors - genetics</subject><subject>Growth Inhibitors - metabolism</subject><subject>Growth Substances - pharmacology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Hormones - pharmacology</subject><subject>Humans</subject><subject>Interleukin-6</subject><subject>Leukemia Inhibitory Factor</subject><subject>Lymphokines - genetics</subject><subject>Lymphokines - metabolism</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pregnancy</subject><subject>Pregnancy, Ectopic - metabolism</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>RNA, Messenger - metabolism</subject><subject>Stromal Cells - metabolism</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO3DAQhq0KBFvaR0DyASF6SLEdx4lPCCFakEAcaCVuluOMu4bEXuwEsW-Pw6722tOMPd9vjz6Ejin5SQkV54-EEFbIsm7OpPhRE0p58fQFLSiRdSEa0eyhxQ45RF9Tep6PTLIDdNDIppZcLtBwH7qp16MLHgeLe5heYHAaO790rRtDXGOrTa74H3jA8L6KkNJMa9_hVQwjOI9bF9Laj0tILuUozh1eToP2Odz3YeVyN04tfEP7-SLB9209Qn9_Xf-5uinuHn7fXl3eFYaXYiygFJy1pmtJS2zJTG2lBW3bpqRcylxFx6ESlhvKNJGVAEaYtqK0sjRc0PIInW7ezQu-TpBGNbhkoO-1hzAlVTeiElU9g9UGNDGkFMGqVXSDjmtFiZo1q0_NanaopFCfmtVTzh1vP5jaAbpdaus1z0-2c52M7m3U3ri0w1jFOaEzdrHBIMt4cxBVMg68gc5FMKPqgvvPIh9WF5xN</recordid><startdate>19961201</startdate><enddate>19961201</enddate><creator>Keltz, Martin D.</creator><creator>Attar, Erkut</creator><creator>Buradagunta, Sumati</creator><creator>Olive, David L.</creator><creator>Kliman, Harvey J.</creator><creator>Arici, Aydin</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961201</creationdate><title>Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube</title><author>Keltz, Martin D. ; Attar, Erkut ; Buradagunta, Sumati ; Olive, David L. ; Kliman, Harvey J. ; Arici, Aydin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-e3642bcdb0b0f32c7f9feafb831499fb86d4e56f4c12a0956e202af63f93c4613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Blood Physiological Phenomena</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Cytokines - pharmacology</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>ectopic pregnancy</topic><topic>Epithelium - metabolism</topic><topic>fallopian tube</topic><topic>Fallopian Tubes - cytology</topic><topic>Fallopian Tubes - metabolism</topic><topic>Fallopian Tubes - physiology</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Growth Inhibitors - genetics</topic><topic>Growth Inhibitors - metabolism</topic><topic>Growth Substances - pharmacology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Hormones - pharmacology</topic><topic>Humans</topic><topic>Interleukin-6</topic><topic>Leukemia Inhibitory Factor</topic><topic>Lymphokines - genetics</topic><topic>Lymphokines - metabolism</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pregnancy</topic><topic>Pregnancy, Ectopic - metabolism</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>RNA, Messenger - metabolism</topic><topic>Stromal Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keltz, Martin D.</creatorcontrib><creatorcontrib>Attar, Erkut</creatorcontrib><creatorcontrib>Buradagunta, Sumati</creatorcontrib><creatorcontrib>Olive, David L.</creatorcontrib><creatorcontrib>Kliman, Harvey J.</creatorcontrib><creatorcontrib>Arici, Aydin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keltz, Martin D.</au><au>Attar, Erkut</au><au>Buradagunta, Sumati</au><au>Olive, David L.</au><au>Kliman, Harvey J.</au><au>Arici, Aydin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1996-12-01</date><risdate>1996</risdate><volume>175</volume><issue>6</issue><spage>1611</spage><epage>1619</epage><pages>1611-1619</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>OBJECTIVE: The fallopian tube is the site of fertilization and early embryonic growth and a common site of ectopic implantation. Although the factors responsible for early embryogenesis and implantation are incompletely understood, leukemia inhibitory factor may have an important role in early embryonic development and implantation. We set out to evaluate the production and modulation of leukemia inhibitory factor in the fallopian tube.
STUDY DESIGN: We first investigated leukemia inhibitory factor messenger ribonucleic acid levels in fallopian tubes. We then investigated leukemia inhibitory factor messenger ribonucleic acid and protein production in tubal epithelial and stromal cell cultures.
RESULTS: Leukemia inhibitory factor messenger ribonucleic acid is expressed in the fallopian tube with only slight variation during the menstrual cycle; however, it is markedly elevated in association with ectopic pregnancy. The level is higher in the tubal mucosa than in the remaining layers and is higher in the more distal segments of the fallopian tube. Estradiol and progesterone did not modulate leukemia inhibitory factor expression in epithelial or stromal cell cultures. Interleukin-1α, tumor necrosis factor-α, and transforming growth factor-β enhanced leukemia inhibitory factor expression in epithelial and stromal cells, with transforming growth factor-β1 enhancing expression by fourfold in stromal cells. Epithelial cells secreted high levels of leukemia inhibitory factor compared with stromal cells (332 ± 89 vs 25 ± 42 pg/mg total protein). Yet stromal cells treated with transforming growth factor-β alone or in combination with epidermal growth factor and platelet-derived growth factor, as well as TNF-α alone or in combination with interleukin-1α enhanced secretion of leukemia inhibitory factor at or above the levels found with epithelial cells.
CONCLUSIONS: We speculate that the high constitutive levels of leukemia inhibitory factor expressed in the ampullary portion of the fallopian tube may play a role in early embryonic development. Additionally, elevated expression with ectopic implantation and the marked induction of secretion in the tubal stroma by growth factors and cytokines suggest a link between inflammation, leukemia inhibitory factor, and tubal ectopic pregnancies. (Am J Obstet Gynecol 1996;175:1611-9.)</abstract><cop>Philadelphia, PA</cop><pub>Mosby, Inc</pub><pmid>8987949</pmid><doi>10.1016/S0002-9378(96)70114-X</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Biological and medical sciences Blood Physiological Phenomena Cells, Cultured Culture Media Cytokines - pharmacology Diseases of mother, fetus and pregnancy ectopic pregnancy Epithelium - metabolism fallopian tube Fallopian Tubes - cytology Fallopian Tubes - metabolism Fallopian Tubes - physiology Female Gene Expression Regulation Growth Inhibitors - genetics Growth Inhibitors - metabolism Growth Substances - pharmacology Gynecology. Andrology. Obstetrics Hormones - pharmacology Humans Interleukin-6 Leukemia Inhibitory Factor Lymphokines - genetics Lymphokines - metabolism Medical sciences Middle Aged Pregnancy Pregnancy, Ectopic - metabolism Pregnancy. Fetus. Placenta RNA, Messenger - metabolism Stromal Cells - metabolism |
| title | Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube |
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