Serological Typing of Acinetobacter calcoaceticus Subspecies anitratus Strains by Soft-Agar Technique

Five factor sera were prepared with strains of Acinetobacter calcoaceticus subspecies anitratus isolated from clinical specimens. With soft-agar technique using their factor sera, 281 (92.1%) out of 305 strains of Ac. anitratus were typable, 63 (22.4%) out of 281 strains reacted with single factor serum and the remaining strains (77.6%) reacted with polyvalent factor sera. Among them, strains reacted with factor sera of the types Ac-80, Ac-132 and Ac-147 reacted 46.1%. Next, higher frequencies of the strains reacting with the factor sera were in order of Ac-80 and Ac-132, Ac-80, Ac-132, Ac-147 and Ac-156, respectively. As the source of clinical specimens excluding blood and bile, the type Ac-80 and Ac-132 were involved in more than 60% of the strains. On the contrary, strains isolated from blood and bile tended to be non-typable by this technique.