Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro
Glycosphingolipid biosynthesis was examined using [3H]-galactose as a precursor as rat L6 myoblasts fused to form multinucleated myotubes. Incorporation of label into neutral glycolipids decreased steadily as the population of myotubes increased, so that final biosynthesis was one-half that observed...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1988-09, Vol.83 (1), p.47-54 |
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| container_title | Molecular and cellular biochemistry |
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| creator | Leskawa, K C Erwin, R E Buse, P E Hogan, E L |
| description | Glycosphingolipid biosynthesis was examined using [3H]-galactose as a precursor as rat L6 myoblasts fused to form multinucleated myotubes. Incorporation of label into neutral glycolipids decreased steadily as the population of myotubes increased, so that final biosynthesis was one-half that observed with myoblasts (p less than 0.02). Conversely, ganglioside biosynthesis doubled during myoblast confluency (p less than 0.02) and then decreased as myotubes formed. Qualitatively, L6 cells synthesized large amounts of ganglioside GM3 during all myogenic phases. The major neutral glycosphingolipid products were lactosylceramide and paragloboside (nLcOse4Cer). Few changes in TLC autoradiographic patterns were noted during differentiation, with the exception of a slight decrease in ganglioside GM1. The results indicate that the biosynthesis of glycosphingolipids is tightly regulated during myogenesis in vitro and suggest a role for membrane gangliosides in muscle cell differentiation. |
| doi_str_mv | 10.1007/BF00223197 |
| format | Article |
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Incorporation of label into neutral glycolipids decreased steadily as the population of myotubes increased, so that final biosynthesis was one-half that observed with myoblasts (p less than 0.02). Conversely, ganglioside biosynthesis doubled during myoblast confluency (p less than 0.02) and then decreased as myotubes formed. Qualitatively, L6 cells synthesized large amounts of ganglioside GM3 during all myogenic phases. The major neutral glycosphingolipid products were lactosylceramide and paragloboside (nLcOse4Cer). Few changes in TLC autoradiographic patterns were noted during differentiation, with the exception of a slight decrease in ganglioside GM1. The results indicate that the biosynthesis of glycosphingolipids is tightly regulated during myogenesis in vitro and suggest a role for membrane gangliosides in muscle cell differentiation.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/BF00223197</identifier><identifier>PMID: 3221840</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Cell Differentiation ; Cells, Cultured ; Chromatography, Thin Layer ; Glycopeptides - biosynthesis ; Glycosphingolipids - biosynthesis ; Glycosphingolipids - isolation & purification ; Kinetics ; Muscles - cytology ; Muscles - metabolism ; myogenesis ; Rats</subject><ispartof>Molecular and cellular biochemistry, 1988-09, Vol.83 (1), p.47-54</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c228t-634fb7a1553dd3cda1d6b2fa4eafe755513fefba39324267e0aaf4dd64267f0f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3221840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leskawa, K C</creatorcontrib><creatorcontrib>Erwin, R E</creatorcontrib><creatorcontrib>Buse, P E</creatorcontrib><creatorcontrib>Hogan, E L</creatorcontrib><title>Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>Glycosphingolipid biosynthesis was examined using [3H]-galactose as a precursor as rat L6 myoblasts fused to form multinucleated myotubes. 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The results indicate that the biosynthesis of glycosphingolipids is tightly regulated during myogenesis in vitro and suggest a role for membrane gangliosides in muscle cell differentiation.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Chromatography, Thin Layer</subject><subject>Glycopeptides - biosynthesis</subject><subject>Glycosphingolipids - biosynthesis</subject><subject>Glycosphingolipids - isolation & purification</subject><subject>Kinetics</subject><subject>Muscles - cytology</subject><subject>Muscles - metabolism</subject><subject>myogenesis</subject><subject>Rats</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUx4Moc04v3oWcPAjVvPxouqMONwcDL3ouaZNskbapSSv0v7dzQ4-eHu99P3x5fBC6BnIPhMiHpyUhlDKYyxM0BSFZwucwP0VTwghJMpDyHF3E-EHIiANM0IRRChknU7ReVUPpY7tzzdZXrnUaF87Hoel2JrqIdR_GBNeD35rm5-ItDqrDmxSXpqoidg3-cl3wl-jMqiqaq-Ocoffl89viJdm8rtaLx01SUpp1Scq4LaQCIZjWrNQKdFpQq7hR1kghBDBrbKHYnFFOU2mIUpZrne4XSyybodtDbxv8Z29il9cu7l9RjfF9zGWWCio5_xcEASLLJB3BuwNYBh9jMDZvg6tVGHIg-V5w_id4hG-OrX1RG_2LHo2yb005dcE</recordid><startdate>198809</startdate><enddate>198809</enddate><creator>Leskawa, K C</creator><creator>Erwin, R E</creator><creator>Buse, P E</creator><creator>Hogan, E L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198809</creationdate><title>Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro</title><author>Leskawa, K C ; Erwin, R E ; Buse, P E ; Hogan, E L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c228t-634fb7a1553dd3cda1d6b2fa4eafe755513fefba39324267e0aaf4dd64267f0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Chromatography, Thin Layer</topic><topic>Glycopeptides - biosynthesis</topic><topic>Glycosphingolipids - biosynthesis</topic><topic>Glycosphingolipids - isolation & purification</topic><topic>Kinetics</topic><topic>Muscles - cytology</topic><topic>Muscles - metabolism</topic><topic>myogenesis</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leskawa, K C</creatorcontrib><creatorcontrib>Erwin, R E</creatorcontrib><creatorcontrib>Buse, P E</creatorcontrib><creatorcontrib>Hogan, E L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leskawa, K C</au><au>Erwin, R E</au><au>Buse, P E</au><au>Hogan, E L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1988-09</date><risdate>1988</risdate><volume>83</volume><issue>1</issue><spage>47</spage><epage>54</epage><pages>47-54</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Glycosphingolipid biosynthesis was examined using [3H]-galactose as a precursor as rat L6 myoblasts fused to form multinucleated myotubes. 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| ispartof | Molecular and cellular biochemistry, 1988-09, Vol.83 (1), p.47-54 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Cell Differentiation Cells, Cultured Chromatography, Thin Layer Glycopeptides - biosynthesis Glycosphingolipids - biosynthesis Glycosphingolipids - isolation & purification Kinetics Muscles - cytology Muscles - metabolism myogenesis Rats |
| title | Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro |
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