Inhibition of nucleoside diphosphate kinase activity by in vitro phosphorylation by protein kinase CK2 Differential phosphorylation of NDP kinases in HeLa cells in culture
Although a number of nucleoside diphosphate kinases (NDPKs) have been reported to act as inhibitors of metastasis or as a transcription factor in mammals, it is not known whether these functions are linked to their enzymatic activity or how this protein is regulated. In this report, we show that in...
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| Veröffentlicht in: | FEBS letters 1996-12, Vol.399 (1), p.183-187 |
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| creator | Biondi, Ricardo M. Engel, Matthias Sauane, Moira Welter, Cornelius Issinger, Olaf-G. Jiménez de Asúa, Luis Passeron, Susana |
| description | Although a number of nucleoside diphosphate kinases (NDPKs) have been reported to act as inhibitors of metastasis or as a transcription factor in mammals, it is not known whether these functions are linked to their enzymatic activity or how this protein is regulated. In this report, we show that in vitro protein kinase CK2 catalyzed phosphorylation of human NDPK A inhibits its enzymatic activity by inhibiting the first step of its ping-pong mechanism of catalysis: its autophosphorylation. Upon in vivo
32P labeling of HeLa cells, we observed that both human NDPKs, A and B, were autophosphorylated on histidine residues, however, only the B isoform appeared to be serine phosphorylated. |
| doi_str_mv | 10.1016/S0014-5793(96)01299-9 |
| format | Article |
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32P labeling of HeLa cells, we observed that both human NDPKs, A and B, were autophosphorylated on histidine residues, however, only the B isoform appeared to be serine phosphorylated.</description><subject>Casein Kinase II</subject><subject>Catalysis</subject><subject>CK2</subject><subject>DMEM</subject><subject>Dulbecco's modified Eagle's medium</subject><subject>Enzyme inhibition</subject><subject>HeLa Cells</subject><subject>Histidine phosphorylation</subject><subject>Humans</subject><subject>NDP kinase</subject><subject>NDPK</subject><subject>nm23</subject><subject>nucleoside diphosphate kinase</subject><subject>Nucleoside-Diphosphate Kinase - antagonists & inhibitors</subject><subject>Nucleoside-Diphosphate Kinase - metabolism</subject><subject>Phosphorylation</subject><subject>Protein kinase CK2</subject><subject>protein kinase CK2 (formerly called casein kinase 2)</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Recombinant Proteins - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUctu1DAUtRBVmRY-oZJXCBahdh4ee4Vg2jIVo4IErC3HudYYMvFgO0X5Jn6yzkPdsKAr-_o87vU9CF1Q8o4Syi6_EULLrFqL4o1gbwnNhcjEM7SifF1kRcn4c7R6pLxAZyH8JKnmVJyiUy54gvgK_b3t9ra20boOO4O7Xrfggm0AN_a4d-G4VxHwL9upAFjpaO9tHHA9YNvhdPUOzyznh1ZNLgk7ehchERbZ5nOOr6wx4KGLVrX_SFLju6uvCz2M1lvYKayhbadK923sPbxEJ0a1AV4t5zn6cXP9fbPNdl8-3W4-7DJd5pXIuFIl5LouyLriphAG8spoQwxjNecNNargIm1Q5aYmmjWqZKKEpgahKlPwujhHr2ff9I_fPYQoDzaMw6gOXB_kmrOCVxVJxGomau9C8GDk0duD8oOkRI4hySkkOSYgBZNTSFIk3cXSoK8P0DyqllQSvp3xP7aF4Wmm8ub6Yz4hIyDY9Dy2ej9bQVrYvQUvg7bQaWisBx1l4-x_hn0AIDi6Sw</recordid><startdate>19961209</startdate><enddate>19961209</enddate><creator>Biondi, Ricardo M.</creator><creator>Engel, Matthias</creator><creator>Sauane, Moira</creator><creator>Welter, Cornelius</creator><creator>Issinger, Olaf-G.</creator><creator>Jiménez de Asúa, Luis</creator><creator>Passeron, Susana</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961209</creationdate><title>Inhibition of nucleoside diphosphate kinase activity by in vitro phosphorylation by protein kinase CK2 Differential phosphorylation of NDP kinases in HeLa cells in culture</title><author>Biondi, Ricardo M. ; Engel, Matthias ; Sauane, Moira ; Welter, Cornelius ; Issinger, Olaf-G. ; Jiménez de Asúa, Luis ; Passeron, Susana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4259-8aa4e2cb30758f39fe25fcf0f66b88d1fa389016a2fb0c6da4694edbe9a5f38b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Casein Kinase II</topic><topic>Catalysis</topic><topic>CK2</topic><topic>DMEM</topic><topic>Dulbecco's modified Eagle's medium</topic><topic>Enzyme inhibition</topic><topic>HeLa Cells</topic><topic>Histidine phosphorylation</topic><topic>Humans</topic><topic>NDP kinase</topic><topic>NDPK</topic><topic>nm23</topic><topic>nucleoside diphosphate kinase</topic><topic>Nucleoside-Diphosphate Kinase - antagonists & inhibitors</topic><topic>Nucleoside-Diphosphate Kinase - metabolism</topic><topic>Phosphorylation</topic><topic>Protein kinase CK2</topic><topic>protein kinase CK2 (formerly called casein kinase 2)</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Recombinant Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biondi, Ricardo M.</creatorcontrib><creatorcontrib>Engel, Matthias</creatorcontrib><creatorcontrib>Sauane, Moira</creatorcontrib><creatorcontrib>Welter, Cornelius</creatorcontrib><creatorcontrib>Issinger, Olaf-G.</creatorcontrib><creatorcontrib>Jiménez de Asúa, Luis</creatorcontrib><creatorcontrib>Passeron, Susana</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biondi, Ricardo M.</au><au>Engel, Matthias</au><au>Sauane, Moira</au><au>Welter, Cornelius</au><au>Issinger, Olaf-G.</au><au>Jiménez de Asúa, Luis</au><au>Passeron, Susana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of nucleoside diphosphate kinase activity by in vitro phosphorylation by protein kinase CK2 Differential phosphorylation of NDP kinases in HeLa cells in culture</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-12-09</date><risdate>1996</risdate><volume>399</volume><issue>1</issue><spage>183</spage><epage>187</epage><pages>183-187</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Although a number of nucleoside diphosphate kinases (NDPKs) have been reported to act as inhibitors of metastasis or as a transcription factor in mammals, it is not known whether these functions are linked to their enzymatic activity or how this protein is regulated. In this report, we show that in vitro protein kinase CK2 catalyzed phosphorylation of human NDPK A inhibits its enzymatic activity by inhibiting the first step of its ping-pong mechanism of catalysis: its autophosphorylation. Upon in vivo
32P labeling of HeLa cells, we observed that both human NDPKs, A and B, were autophosphorylated on histidine residues, however, only the B isoform appeared to be serine phosphorylated.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8980148</pmid><doi>10.1016/S0014-5793(96)01299-9</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Casein Kinase II Catalysis CK2 DMEM Dulbecco's modified Eagle's medium Enzyme inhibition HeLa Cells Histidine phosphorylation Humans NDP kinase NDPK nm23 nucleoside diphosphate kinase Nucleoside-Diphosphate Kinase - antagonists & inhibitors Nucleoside-Diphosphate Kinase - metabolism Phosphorylation Protein kinase CK2 protein kinase CK2 (formerly called casein kinase 2) Protein-Serine-Threonine Kinases - metabolism Recombinant Proteins - metabolism |
| title | Inhibition of nucleoside diphosphate kinase activity by in vitro phosphorylation by protein kinase CK2 Differential phosphorylation of NDP kinases in HeLa cells in culture |
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