Multicopy expression vector based on temperature-regulated lac repressor: expression of human immunodeficiency virus env gene in Escherichia coli
A new expression vector (pBB1) has been constructed for the regulated expression of genes in Escherichia coli. Based on the pUC plasmids, the pBB 1 carries lacIts allele of the lac repressor gene. This makes it possible to control expression of cloned genes by shifting the temperature from 30°C to 4...
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| Veröffentlicht in: | Gene 1988-10, Vol.70 (2), p.415-417 |
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| container_title | Gene |
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| creator | Bukrinsky, Michail I. Barsov, Eugene V. Shilov, Alexander A. |
| description | A new expression vector (pBB1) has been constructed for the regulated expression of genes in
Escherichia coli. Based on the pUC plasmids, the pBB 1 carries
lacIts allele of the
lac repressor gene. This makes it possible to control expression of cloned genes by shifting the temperature from 30°C to 42°C. Thus the vector combines advantages of the pUC plasmids with convenient regulation by temperature. Expression of a fragment of HIV-1
env gene was achieved with the help of this vector and shown by enzyme-linked immunosorbent assay and Western-blot analysis. |
| doi_str_mv | 10.1016/0378-1119(88)90215-6 |
| format | Article |
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Escherichia coli. Based on the pUC plasmids, the pBB 1 carries
lacIts allele of the
lac repressor gene. This makes it possible to control expression of cloned genes by shifting the temperature from 30°C to 42°C. Thus the vector combines advantages of the pUC plasmids with convenient regulation by temperature. Expression of a fragment of HIV-1
env gene was achieved with the help of this vector and shown by enzyme-linked immunosorbent assay and Western-blot analysis.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(88)90215-6</identifier><identifier>PMID: 3063609</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>AIDS/HIV ; Biological and medical sciences ; Biotechnology ; Blotting, Western ; cloning vectors ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; gene fusion ; genes ; Genetic engineering ; Genetic technics ; Genetic Vectors ; HIV Antigens - biosynthesis ; HIV Antigens - genetics ; human immunodeficiency virus ; lacIts allele ; Methods. Procedures. Technologies ; Molecular cloning ; plasmids ; pUC plasmids ; Recombinant DNA ; Recombinant Fusion Proteins - genetics ; Repressor Proteins - genetics ; Temperature ; Transcription Factors - genetics ; Viral Envelope Proteins - biosynthesis ; Viral Envelope Proteins - genetics ; Western blot</subject><ispartof>Gene, 1988-10, Vol.70 (2), p.415-417</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-dddb0daf5b258010b18bb718ef1dd3ccc83357135b76fc34a6e897835c16a7b53</citedby><cites>FETCH-LOGICAL-c453t-dddb0daf5b258010b18bb718ef1dd3ccc83357135b76fc34a6e897835c16a7b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(88)90215-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7341641$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3063609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bukrinsky, Michail I.</creatorcontrib><creatorcontrib>Barsov, Eugene V.</creatorcontrib><creatorcontrib>Shilov, Alexander A.</creatorcontrib><title>Multicopy expression vector based on temperature-regulated lac repressor: expression of human immunodeficiency virus env gene in Escherichia coli</title><title>Gene</title><addtitle>Gene</addtitle><description>A new expression vector (pBB1) has been constructed for the regulated expression of genes in
Escherichia coli. Based on the pUC plasmids, the pBB 1 carries
lacIts allele of the
lac repressor gene. This makes it possible to control expression of cloned genes by shifting the temperature from 30°C to 42°C. Thus the vector combines advantages of the pUC plasmids with convenient regulation by temperature. Expression of a fragment of HIV-1
env gene was achieved with the help of this vector and shown by enzyme-linked immunosorbent assay and Western-blot analysis.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>cloning vectors</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>gene fusion</subject><subject>genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>HIV Antigens - biosynthesis</subject><subject>HIV Antigens - genetics</subject><subject>human immunodeficiency virus</subject><subject>lacIts allele</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>plasmids</subject><subject>pUC plasmids</subject><subject>Recombinant DNA</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>Temperature</subject><subject>Transcription Factors - genetics</subject><subject>Viral Envelope Proteins - biosynthesis</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Western blot</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuKFDEUhoMoY8_oGyhkIaKL0pxO5VIuBBlmVBhxo-uQSk5NR6oqZVLV2I_hG5u-0LjSbEL4v_8QzkfIM2BvgIF8y7jSFQA0r7R-3bA1iEo-ICvQqqkY4_ohWZ2Rx-Qy5x-sHCHWF-SCM8kla1bk95eln4OL047irylhziGOdItujom2NqOn5T3jMGGy85KwSni_9HYuQW8dTXgoxfTu737s6GYZ7EjDMCxj9NgFF3B0O7oNackUxy29xxFpGOlNdhtMwW2CpS724Ql51Nk-49PTfUW-3958u_5U3X39-Pn6w13lasHnynvfMm870a6FZsBa0G2rQGMH3nPnnOZcKOCiVbJzvLYSdaM0Fw6kVa3gV-Tlce6U4s8F82yGkB32vR0xLtkoLddKCP5fEARoKbUqYH0EXYo5J-zMlMJg084AM3tlZu_D7H0Yrc1BmZGl9vw0f2kH9OfSyVHJX5xym53tu2RHF_IZU7wGWUPB3h8xLEvbBkwmH3aOPqSi0_gY_v2PP_vDtfw</recordid><startdate>19881030</startdate><enddate>19881030</enddate><creator>Bukrinsky, Michail I.</creator><creator>Barsov, Eugene V.</creator><creator>Shilov, Alexander A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19881030</creationdate><title>Multicopy expression vector based on temperature-regulated lac repressor: expression of human immunodeficiency virus env gene in Escherichia coli</title><author>Bukrinsky, Michail I. ; Barsov, Eugene V. ; Shilov, Alexander A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-dddb0daf5b258010b18bb718ef1dd3ccc83357135b76fc34a6e897835c16a7b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>cloning vectors</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>gene fusion</topic><topic>genes</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>HIV Antigens - biosynthesis</topic><topic>HIV Antigens - genetics</topic><topic>human immunodeficiency virus</topic><topic>lacIts allele</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>plasmids</topic><topic>pUC plasmids</topic><topic>Recombinant DNA</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Repressor Proteins - genetics</topic><topic>Temperature</topic><topic>Transcription Factors - genetics</topic><topic>Viral Envelope Proteins - biosynthesis</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Western blot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bukrinsky, Michail I.</creatorcontrib><creatorcontrib>Barsov, Eugene V.</creatorcontrib><creatorcontrib>Shilov, Alexander A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bukrinsky, Michail I.</au><au>Barsov, Eugene V.</au><au>Shilov, Alexander A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multicopy expression vector based on temperature-regulated lac repressor: expression of human immunodeficiency virus env gene in Escherichia coli</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1988-10-30</date><risdate>1988</risdate><volume>70</volume><issue>2</issue><spage>415</spage><epage>417</epage><pages>415-417</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>A new expression vector (pBB1) has been constructed for the regulated expression of genes in
Escherichia coli. Based on the pUC plasmids, the pBB 1 carries
lacIts allele of the
lac repressor gene. This makes it possible to control expression of cloned genes by shifting the temperature from 30°C to 42°C. Thus the vector combines advantages of the pUC plasmids with convenient regulation by temperature. Expression of a fragment of HIV-1
env gene was achieved with the help of this vector and shown by enzyme-linked immunosorbent assay and Western-blot analysis.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3063609</pmid><doi>10.1016/0378-1119(88)90215-6</doi><tpages>3</tpages></addata></record> |
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| ispartof | Gene, 1988-10, Vol.70 (2), p.415-417 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | AIDS/HIV Biological and medical sciences Biotechnology Blotting, Western cloning vectors Enzyme-Linked Immunosorbent Assay Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation gene fusion genes Genetic engineering Genetic technics Genetic Vectors HIV Antigens - biosynthesis HIV Antigens - genetics human immunodeficiency virus lacIts allele Methods. Procedures. Technologies Molecular cloning plasmids pUC plasmids Recombinant DNA Recombinant Fusion Proteins - genetics Repressor Proteins - genetics Temperature Transcription Factors - genetics Viral Envelope Proteins - biosynthesis Viral Envelope Proteins - genetics Western blot |
| title | Multicopy expression vector based on temperature-regulated lac repressor: expression of human immunodeficiency virus env gene in Escherichia coli |
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