Immunological properties of an N-terminal fragment of herpes simplex virus type 1 glycoprotein D expressed in Escherichia coli (brief report)
The N-terminal fragment, comprising residues -5 to 55 of herpes simplex virus type 1 glycoprotein D was expressed as a beta-galactosidase fusion protein in Escherichia coli. This gD-fusion protein reacts with monoclonal antibody LP 14 directed against glycoprotein D of HSV. Antisera obtained after i...
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| Veröffentlicht in: | Archives of virology 1988-01, Vol.103 (3-4), p.267-274 |
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| container_end_page | 274 |
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| container_issue | 3-4 |
| container_start_page | 267 |
| container_title | Archives of virology |
| container_volume | 103 |
| creator | KOCKEN, C. H. M GEERLIGS, H. J BOS, C. A AG, B WEIJER, W. J DRIJFHOUT, J. W WELLING, G. W WELLING-WESTER, S |
| description | The N-terminal fragment, comprising residues -5 to 55 of herpes simplex virus type 1 glycoprotein D was expressed as a beta-galactosidase fusion protein in Escherichia coli. This gD-fusion protein reacts with monoclonal antibody LP 14 directed against glycoprotein D of HSV. Antisera obtained after immunization of rabbits with purified gD-fusion protein react with HSV-1 gD in a Western blot and with N-terminal synthetic peptides of gD. In addition, these antisera are able to neutralize viral infectivity in vitro. |
| doi_str_mv | 10.1007/BF01311098 |
| format | Article |
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Psychology ; Microbiology ; Molecular Sequence Data ; Plasmids ; Rabbits ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - immunology ; Recombinant Proteins - immunology ; Simplexvirus - genetics ; Simplexvirus - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Viral Envelope Proteins - biosynthesis ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Virology</subject><ispartof>Archives of virology, 1988-01, Vol.103 (3-4), p.267-274</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-5fc2cf40ccb2065e0c1bb138edcc494ab3910540fe3fefd3cc8a0d2d2ba3746b3</citedby><cites>FETCH-LOGICAL-c378t-5fc2cf40ccb2065e0c1bb138edcc494ab3910540fe3fefd3cc8a0d2d2ba3746b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7266689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2850785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KOCKEN, C. 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Antisera obtained after immunization of rabbits with purified gD-fusion protein react with HSV-1 gD in a Western blot and with N-terminal synthetic peptides of gD. In addition, these antisera are able to neutralize viral infectivity in vitro.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibodies, Viral - immunology</subject><subject>beta-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Chinchilla</subject><subject>Cloning, Molecular</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Rabbits</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Viral Envelope Proteins - biosynthesis</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFO3TAQRa2KCh7QTfeVvEAVrRQYx3HiLAuFFgnRTVlHzmT8MEri1E4Q7yP4Z4x4oktW1vieubJ8GPss4EQAVKdnlyCkEFDrD2wlCplnuqr1DluBhCLTJeg9th_jPUC6kGqX7eZaQaXVij1dDcMy-t6vHZqeT8FPFGZHkXvLzchvspnC4MaU2WDWA43zS3JHYUpMdMPU0yN_cGGJfN5MxAVf9xv0qWgmN_KfnB6nQDFSx9N4ETGtOrxzhqPvHT9ugyPLA00-zN8O2Udr-kiftucBu728-Hv-O7v-8-vq_Md1hrLSc6Ys5mgLQGxzKBUBirYVUlOHWNSFaWUtQBVgSVqynUTUBrq8y1sjq6Js5QH7-tqbnvlvoTg3g4tIfW9G8ktsKl3mqlTyXVAoWRfJQQK_v4IYfIyBbDMFN5iwaQQ0L5Ka_5IS_GXburQDdW_o1krKj7a5iclK-vgRXXzDqrwsS13LZ7mCm38</recordid><startdate>19880101</startdate><enddate>19880101</enddate><creator>KOCKEN, C. H. M</creator><creator>GEERLIGS, H. J</creator><creator>BOS, C. A</creator><creator>AG, B</creator><creator>WEIJER, W. J</creator><creator>DRIJFHOUT, J. W</creator><creator>WELLING, G. W</creator><creator>WELLING-WESTER, S</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19880101</creationdate><title>Immunological properties of an N-terminal fragment of herpes simplex virus type 1 glycoprotein D expressed in Escherichia coli (brief report)</title><author>KOCKEN, C. H. M ; GEERLIGS, H. J ; BOS, C. A ; AG, B ; WEIJER, W. J ; DRIJFHOUT, J. W ; WELLING, G. W ; WELLING-WESTER, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-5fc2cf40ccb2065e0c1bb138edcc494ab3910540fe3fefd3cc8a0d2d2ba3746b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibodies, Viral - immunology</topic><topic>beta-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Chinchilla</topic><topic>Cloning, Molecular</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Rabbits</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Viral Envelope Proteins - biosynthesis</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOCKEN, C. H. M</creatorcontrib><creatorcontrib>GEERLIGS, H. J</creatorcontrib><creatorcontrib>BOS, C. A</creatorcontrib><creatorcontrib>AG, B</creatorcontrib><creatorcontrib>WEIJER, W. J</creatorcontrib><creatorcontrib>DRIJFHOUT, J. W</creatorcontrib><creatorcontrib>WELLING, G. W</creatorcontrib><creatorcontrib>WELLING-WESTER, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOCKEN, C. H. M</au><au>GEERLIGS, H. J</au><au>BOS, C. A</au><au>AG, B</au><au>WEIJER, W. J</au><au>DRIJFHOUT, J. W</au><au>WELLING, G. W</au><au>WELLING-WESTER, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunological properties of an N-terminal fragment of herpes simplex virus type 1 glycoprotein D expressed in Escherichia coli (brief report)</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1988-01-01</date><risdate>1988</risdate><volume>103</volume><issue>3-4</issue><spage>267</spage><epage>274</epage><pages>267-274</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>The N-terminal fragment, comprising residues -5 to 55 of herpes simplex virus type 1 glycoprotein D was expressed as a beta-galactosidase fusion protein in Escherichia coli. This gD-fusion protein reacts with monoclonal antibody LP 14 directed against glycoprotein D of HSV. Antisera obtained after immunization of rabbits with purified gD-fusion protein react with HSV-1 gD in a Western blot and with N-terminal synthetic peptides of gD. In addition, these antisera are able to neutralize viral infectivity in vitro.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>2850785</pmid><doi>10.1007/BF01311098</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Archives of virology, 1988-01, Vol.103 (3-4), p.267-274 |
| issn | 0304-8608 1432-8798 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amino Acid Sequence Animals Antibodies, Viral - biosynthesis Antibodies, Viral - immunology beta-Galactosidase - genetics Biological and medical sciences Chinchilla Cloning, Molecular Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Microbiology Molecular Sequence Data Plasmids Rabbits Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - immunology Recombinant Proteins - immunology Simplexvirus - genetics Simplexvirus - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Viral Envelope Proteins - biosynthesis Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Virology |
| title | Immunological properties of an N-terminal fragment of herpes simplex virus type 1 glycoprotein D expressed in Escherichia coli (brief report) |
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