Sequential Estimation of Linkage between PCR-Generated Markers and a Target Gene Employing Stepwise Bulked Analysis
PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a cens...
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| Veröffentlicht in: | Biometrics 1996-12, Vol.52 (4), p.1428-1439 |
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| container_end_page | 1439 |
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| container_issue | 4 |
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| container_title | Biometrics |
| container_volume | 52 |
| creator | Ronin, Yefim I. Korol, Abraham B. Fahima, Tzion Kirzhner, Valery M. Nevo, Eviatar |
| description | PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a censored estimation of the recombination rate. The sequential estimation is compared to fixed sample size design. In both cases, the proposed procedure can achieve a substantial reduction in the number of PCR runs (up to 90-97% for close linkage) as compared to the standard individual-by-individual analysis. |
| doi_str_mv | 10.2307/2532856 |
| format | Article |
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| identifier | ISSN: 0006-341X |
| ispartof | Biometrics, 1996-12, Vol.52 (4), p.1428-1439 |
| issn | 0006-341X 1541-0420 |
| language | eng |
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| source | MEDLINE; JSTOR Mathematics & Statistics; JSTOR Archive Collection A-Z Listing |
| subjects | Analytical estimating Biometrics Biometry - methods Censorship Chromosome Mapping Computer Simulation Corporate censorship Data Interpretation, Statistical DNA Genes, Plant Genetic Linkage Genetic loci Genetic Markers Genotypes Human genetics Polymerase chain reaction Polymerase Chain Reaction - statistics & numerical data Recombination, Genetic Sample size |
| title | Sequential Estimation of Linkage between PCR-Generated Markers and a Target Gene Employing Stepwise Bulked Analysis |
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