Peculiarities of gradient ion-exchange high-performance liquid chromatography of proteins

Since the influence of column length on protein resolution in high-performance liquid chromatography (HPLC) is not clear, different viewpoints presented in the literature are analysed in detail. The influence of gradient steepness on the length of the working column part ( X 0) or the part of a colu...

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Veröffentlicht in:	Journal of Chromatography A 1996-11, Vol.753 (2), p.217-225
Hauptverfasser:	Dubinina, N.I., Kurenbin, O.I., Tennikova, T.B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid - methods Chromatography, Ion Exchange - methods Column length Gradient elution Proteins Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
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