The role of ADP in endotoxin-induced equine platelet activation
We have shown previously that endotoxin induces platelet aggregation in equine heparinised whole blood in a platelet-activating factor (PAF; 1- O-alkyl-2-acetyl- sn-glycero-3-phosphocholine) dependent manner. ADP is an agonist of platelets and is present in platelet dense granules with ATP in high c...
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| Veröffentlicht in: | European journal of pharmacology 1996-11, Vol.315 (2), p.203-212 |
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| container_title | European journal of pharmacology |
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| creator | Jarvis, Gavin E. Evans, Richard J. Heath, M.Fred |
| description | We have shown previously that endotoxin induces platelet aggregation in equine heparinised whole blood in a platelet-activating factor (PAF; 1-
O-alkyl-2-acetyl-
sn-glycero-3-phosphocholine) dependent manner. ADP is an agonist of platelets and is present in platelet dense granules with ATP in high concentrations. An investigation was carried out to establish whether endotoxin-induced platelet activation was associated with release of platelet ATP and ADP. ADP-scavenging enzyme systems significantly inhibited endotoxin-induced aggregation. Plasma levels of adenine nucleotides were measured using a luminometric assay following incubation of heparinised equine whole blood with endotoxin (300 ng/ml). After addition of endotoxin, ATP and ADP were released from the platelets and then subsequently degraded to AMP. WEB2086 (4-{3-[4-(
o-chlorophenyl)-9-methyl-6
H-thieno[3,2,-
f]-
s-triazolo[4,3-
a][1,4]diazepin-2-yl]proprionyl}-morpholine) (100 nM), a competitive PAF receptor antagonist, inhibited endotoxin-induced aggregation and also inhibited the release of adenine nucleotides from the platelets. It is concluded that endotoxin-induced aggregation is dependent upon ADP released from platelet dense granules. |
| doi_str_mv | 10.1016/S0014-2999(96)00637-1 |
| format | Article |
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O-alkyl-2-acetyl-
sn-glycero-3-phosphocholine) dependent manner. ADP is an agonist of platelets and is present in platelet dense granules with ATP in high concentrations. An investigation was carried out to establish whether endotoxin-induced platelet activation was associated with release of platelet ATP and ADP. ADP-scavenging enzyme systems significantly inhibited endotoxin-induced aggregation. Plasma levels of adenine nucleotides were measured using a luminometric assay following incubation of heparinised equine whole blood with endotoxin (300 ng/ml). After addition of endotoxin, ATP and ADP were released from the platelets and then subsequently degraded to AMP. WEB2086 (4-{3-[4-(
o-chlorophenyl)-9-methyl-6
H-thieno[3,2,-
f]-
s-triazolo[4,3-
a][1,4]diazepin-2-yl]proprionyl}-morpholine) (100 nM), a competitive PAF receptor antagonist, inhibited endotoxin-induced aggregation and also inhibited the release of adenine nucleotides from the platelets. It is concluded that endotoxin-induced aggregation is dependent upon ADP released from platelet dense granules.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/S0014-2999(96)00637-1</identifier><identifier>PMID: 8960885</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adenine nucleotide ; Adenosine Diphosphate - metabolism ; Adenosine Diphosphate - physiology ; Adenosine Monophosphate - metabolism ; Adenosine Triphosphate - metabolism ; Animals ; Apyrase - pharmacology ; Biological and medical sciences ; Blood coagulation. Blood cells ; Blood Platelets - metabolism ; Endotoxin ; Endotoxins - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Horses ; Molecular and cellular biology ; PAF (platelet-activating factor) ; Platelet ; Platelet Activating Factor - physiology ; Platelet Activation - drug effects ; Platelet aggregation ; Platelet Aggregation - drug effects ; Spectrophotometry</subject><ispartof>European journal of pharmacology, 1996-11, Vol.315 (2), p.203-212</ispartof><rights>1996</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-910d4bcd9b7552bcd92e443c8290cbb0dd4bed8ef3a88fea84453b83495f00413</citedby><cites>FETCH-LOGICAL-c389t-910d4bcd9b7552bcd92e443c8290cbb0dd4bed8ef3a88fea84453b83495f00413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-2999(96)00637-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2515368$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8960885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jarvis, Gavin E.</creatorcontrib><creatorcontrib>Evans, Richard J.</creatorcontrib><creatorcontrib>Heath, M.Fred</creatorcontrib><title>The role of ADP in endotoxin-induced equine platelet activation</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>We have shown previously that endotoxin induces platelet aggregation in equine heparinised whole blood in a platelet-activating factor (PAF; 1-
O-alkyl-2-acetyl-
sn-glycero-3-phosphocholine) dependent manner. ADP is an agonist of platelets and is present in platelet dense granules with ATP in high concentrations. An investigation was carried out to establish whether endotoxin-induced platelet activation was associated with release of platelet ATP and ADP. ADP-scavenging enzyme systems significantly inhibited endotoxin-induced aggregation. Plasma levels of adenine nucleotides were measured using a luminometric assay following incubation of heparinised equine whole blood with endotoxin (300 ng/ml). After addition of endotoxin, ATP and ADP were released from the platelets and then subsequently degraded to AMP. WEB2086 (4-{3-[4-(
o-chlorophenyl)-9-methyl-6
H-thieno[3,2,-
f]-
s-triazolo[4,3-
a][1,4]diazepin-2-yl]proprionyl}-morpholine) (100 nM), a competitive PAF receptor antagonist, inhibited endotoxin-induced aggregation and also inhibited the release of adenine nucleotides from the platelets. It is concluded that endotoxin-induced aggregation is dependent upon ADP released from platelet dense granules.</description><subject>Adenine nucleotide</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Diphosphate - physiology</subject><subject>Adenosine Monophosphate - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Apyrase - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - metabolism</subject><subject>Endotoxin</subject><subject>Endotoxins - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Horses</subject><subject>Molecular and cellular biology</subject><subject>PAF (platelet-activating factor)</subject><subject>Platelet</subject><subject>Platelet Activating Factor - physiology</subject><subject>Platelet Activation - drug effects</subject><subject>Platelet aggregation</subject><subject>Platelet Aggregation - drug effects</subject><subject>Spectrophotometry</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMoOl4eQehCRBfVk6Zpk5WIdxhQUNchTU4x0knGpBV9ezsXZuvqHPi_c-Ej5JjCBQVaXb4C0DIvpJRnsjoHqFid0y0yoaKWOdS02CaTDbJH9lP6BAAuC75LdoWsQAg-IVdvH5jF0GEW2uz69iVzPkNvQx9-nM-dt4NBm-HX4Dxm80732GGfadO7b9274A_JTqu7hEfrekDe7-_ebh7z6fPD0831NDdMyD6XFGzZGCubmvNi0RRYlsyIQoJpGrBjilZgy7QQLWpRlpw1gpWStwAlZQfkdLV3HsPXgKlXM5cMdp32GIakalEVbORHkK9AE0NKEVs1j26m46-ioBbi1FKcWlhRslJLcWpx4Hh9YGhmaDdTa1NjfrLOdTK6a6P2xqUNVnDKWSVG7GqF4Sjj22FUyTj0o0QX0fTKBvfPI3-3VYlj</recordid><startdate>19961114</startdate><enddate>19961114</enddate><creator>Jarvis, Gavin E.</creator><creator>Evans, Richard J.</creator><creator>Heath, M.Fred</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961114</creationdate><title>The role of ADP in endotoxin-induced equine platelet activation</title><author>Jarvis, Gavin E. ; Evans, Richard J. ; Heath, M.Fred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-910d4bcd9b7552bcd92e443c8290cbb0dd4bed8ef3a88fea84453b83495f00413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adenine nucleotide</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Diphosphate - physiology</topic><topic>Adenosine Monophosphate - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Apyrase - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - metabolism</topic><topic>Endotoxin</topic><topic>Endotoxins - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Horses</topic><topic>Molecular and cellular biology</topic><topic>PAF (platelet-activating factor)</topic><topic>Platelet</topic><topic>Platelet Activating Factor - physiology</topic><topic>Platelet Activation - drug effects</topic><topic>Platelet aggregation</topic><topic>Platelet Aggregation - drug effects</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jarvis, Gavin E.</creatorcontrib><creatorcontrib>Evans, Richard J.</creatorcontrib><creatorcontrib>Heath, M.Fred</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jarvis, Gavin E.</au><au>Evans, Richard J.</au><au>Heath, M.Fred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of ADP in endotoxin-induced equine platelet activation</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1996-11-14</date><risdate>1996</risdate><volume>315</volume><issue>2</issue><spage>203</spage><epage>212</epage><pages>203-212</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>We have shown previously that endotoxin induces platelet aggregation in equine heparinised whole blood in a platelet-activating factor (PAF; 1-
O-alkyl-2-acetyl-
sn-glycero-3-phosphocholine) dependent manner. ADP is an agonist of platelets and is present in platelet dense granules with ATP in high concentrations. An investigation was carried out to establish whether endotoxin-induced platelet activation was associated with release of platelet ATP and ADP. ADP-scavenging enzyme systems significantly inhibited endotoxin-induced aggregation. Plasma levels of adenine nucleotides were measured using a luminometric assay following incubation of heparinised equine whole blood with endotoxin (300 ng/ml). After addition of endotoxin, ATP and ADP were released from the platelets and then subsequently degraded to AMP. WEB2086 (4-{3-[4-(
o-chlorophenyl)-9-methyl-6
H-thieno[3,2,-
f]-
s-triazolo[4,3-
a][1,4]diazepin-2-yl]proprionyl}-morpholine) (100 nM), a competitive PAF receptor antagonist, inhibited endotoxin-induced aggregation and also inhibited the release of adenine nucleotides from the platelets. It is concluded that endotoxin-induced aggregation is dependent upon ADP released from platelet dense granules.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8960885</pmid><doi>10.1016/S0014-2999(96)00637-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | European journal of pharmacology, 1996-11, Vol.315 (2), p.203-212 |
| issn | 0014-2999 1879-0712 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adenine nucleotide Adenosine Diphosphate - metabolism Adenosine Diphosphate - physiology Adenosine Monophosphate - metabolism Adenosine Triphosphate - metabolism Animals Apyrase - pharmacology Biological and medical sciences Blood coagulation. Blood cells Blood Platelets - metabolism Endotoxin Endotoxins - pharmacology Female Fundamental and applied biological sciences. Psychology Horses Molecular and cellular biology PAF (platelet-activating factor) Platelet Platelet Activating Factor - physiology Platelet Activation - drug effects Platelet aggregation Platelet Aggregation - drug effects Spectrophotometry |
| title | The role of ADP in endotoxin-induced equine platelet activation |
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