Sphingosine and psychosine, suggested inhibitors of protein kinase C, inhibit LH effects in rat luteal cells
The possible involvement of protein kinase C on luteinizing hormone (LH) effects in dispersed rat luteal cells was investigated using two substances that have been reported to be protein kinase C inhibitors, sphingosine and psychosine. Sphingosine efficiently inhibited protein kinase C activity both...
Gespeichert in:
| Veröffentlicht in: | Molecular and cellular endocrinology 1988-12, Vol.60 (2), p.127-135 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 135 |
|---|---|
| container_issue | 2 |
| container_start_page | 127 |
| container_title | Molecular and cellular endocrinology |
| container_volume | 60 |
| creator | Sender Baum, Monica G. Ahrén, Kurt E.B. |
| description | The possible involvement of protein kinase C on luteinizing hormone (LH) effects in dispersed rat luteal cells was investigated using two substances that have been reported to be protein kinase C inhibitors, sphingosine and psychosine. Sphingosine efficiently inhibited protein kinase C activity both in brain and luteal cytosol fractions. Both substances inhibited LH-stimulated cyclic adenosine monophosphate (cAMP) accumulation in a dose-dependent fashion with an ID
50 at 3–7 μM (sphingosine) and 40 μ.m (psychosine). LH-stimulated progesterone production was also inhibited with an ID
50 at 6–10 μM (sphingosine) and 40–100 μM (psychosine). The inhibition was not due to an increased phosphodiesterase activity since IBMX (3-isobutyl-1-methylxanthine, 0.1 mM) and RO 20–1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone, 0.1 mM) did not abolish the inhibitory effect of sphingosine. To study the mode of action of sphingosine, forskolin and cAMP analogues were tested. The effect of these substances on Steroidogenesis was inhibited, as well as the forskolin-induced cAMP accumulation, by sphingosine.
This study demonstrates a clear inhibition of LH-stimulated effects by sphingosine and psychosine. LH action in rat luteal cells is discussed in relation to protein kinase C and the possible mode of sphingosine action. |
| doi_str_mv | 10.1016/0303-7207(88)90171-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78623570</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0303720788901712</els_id><sourcerecordid>15148406</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-a5f4595506ddf3ddf3fca2315a57db166c57504518ff1b61397480a83ee1858d3</originalsourceid><addsrcrecordid>eNqFUU1v1DAQtRBVWVr-AUi-gEBqih3bsXNBQqt-IK3EAThbXnu8a8g6iydB6r8n6S7trRxG1sx78_T8hpDXnF1yxpuPTDBR6Zrp98Z8aBnXvKqfkQU3uq4MU_o5WTxQXpCXiD8ZY1rV5pSc1rIRrRQL0n3bb1Pe9JgyUJcD3eOd3963FxTHzQZwgEBT3qZ1GvqCtI90X_oBUqa_UnYIdHnxD6erWwoxgh9wGtHiBtqNA7iOeug6PCcn0XUIr47vGflxffV9eVutvt58WX5eVV5yM1RORalapVgTQhRzRe9qwZVTOqx503ilFZOKmxj5uuGi1dIwZwQAN8oEcUbeHXQno7_H6Qd2l3B24DL0I1ptmloozf5L5IpLI1kzEeWB6EuPWCDafUk7V-4sZ3a-hp2jtnPU1hh7fw1bT2tvjvrjegfhYekY_4S_PeIOveticdknfNRudWt0O_v8dODBlNqfBMWiT5A9hFSmsG3o09NG_gIr_qXH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15148406</pqid></control><display><type>article</type><title>Sphingosine and psychosine, suggested inhibitors of protein kinase C, inhibit LH effects in rat luteal cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Sender Baum, Monica G. ; Ahrén, Kurt E.B.</creator><creatorcontrib>Sender Baum, Monica G. ; Ahrén, Kurt E.B.</creatorcontrib><description>The possible involvement of protein kinase C on luteinizing hormone (LH) effects in dispersed rat luteal cells was investigated using two substances that have been reported to be protein kinase C inhibitors, sphingosine and psychosine. Sphingosine efficiently inhibited protein kinase C activity both in brain and luteal cytosol fractions. Both substances inhibited LH-stimulated cyclic adenosine monophosphate (cAMP) accumulation in a dose-dependent fashion with an ID
50 at 3–7 μM (sphingosine) and 40 μ.m (psychosine). LH-stimulated progesterone production was also inhibited with an ID
50 at 6–10 μM (sphingosine) and 40–100 μM (psychosine). The inhibition was not due to an increased phosphodiesterase activity since IBMX (3-isobutyl-1-methylxanthine, 0.1 mM) and RO 20–1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone, 0.1 mM) did not abolish the inhibitory effect of sphingosine. To study the mode of action of sphingosine, forskolin and cAMP analogues were tested. The effect of these substances on Steroidogenesis was inhibited, as well as the forskolin-induced cAMP accumulation, by sphingosine.
This study demonstrates a clear inhibition of LH-stimulated effects by sphingosine and psychosine. LH action in rat luteal cells is discussed in relation to protein kinase C and the possible mode of sphingosine action.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/0303-7207(88)90171-2</identifier><identifier>PMID: 2463943</identifier><identifier>CODEN: MCEND6</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>1-Methyl-3-isobutylxanthine - pharmacology ; 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology ; 8-Bromo Cyclic Adenosine Monophosphate - pharmacology ; Animals ; Biological and medical sciences ; Brain - drug effects ; Brain - metabolism ; Bucladesine - pharmacology ; Colforsin - pharmacology ; Corpus Luteum - metabolism ; Cyclic AMP ; Cyclic AMP - biosynthesis ; Cytosol - metabolism ; Dose-Response Relationship, Drug ; Female ; Fundamental and applied biological sciences. Psychology ; Luteal Cells - drug effects ; Luteal Cells - metabolism ; Luteinizing hormone ; Luteinizing Hormone - antagonists & inhibitors ; Luteinizing Hormone - pharmacology ; Progesterone - biosynthesis ; Protein kinase C ; Protein Kinase C - antagonists & inhibitors ; Psychosine ; Psychosine - pharmacology ; Rat luteal cells ; Rats ; Rats, Inbred Strains ; Sphingosine ; Sphingosine - analogs & derivatives ; Sphingosine - pharmacology ; Steroidogenesis ; Vertebrates: endocrinology</subject><ispartof>Molecular and cellular endocrinology, 1988-12, Vol.60 (2), p.127-135</ispartof><rights>1988</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-a5f4595506ddf3ddf3fca2315a57db166c57504518ff1b61397480a83ee1858d3</citedby><cites>FETCH-LOGICAL-c418t-a5f4595506ddf3ddf3fca2315a57db166c57504518ff1b61397480a83ee1858d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0303720788901712$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19798790$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2463943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sender Baum, Monica G.</creatorcontrib><creatorcontrib>Ahrén, Kurt E.B.</creatorcontrib><title>Sphingosine and psychosine, suggested inhibitors of protein kinase C, inhibit LH effects in rat luteal cells</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>The possible involvement of protein kinase C on luteinizing hormone (LH) effects in dispersed rat luteal cells was investigated using two substances that have been reported to be protein kinase C inhibitors, sphingosine and psychosine. Sphingosine efficiently inhibited protein kinase C activity both in brain and luteal cytosol fractions. Both substances inhibited LH-stimulated cyclic adenosine monophosphate (cAMP) accumulation in a dose-dependent fashion with an ID
50 at 3–7 μM (sphingosine) and 40 μ.m (psychosine). LH-stimulated progesterone production was also inhibited with an ID
50 at 6–10 μM (sphingosine) and 40–100 μM (psychosine). The inhibition was not due to an increased phosphodiesterase activity since IBMX (3-isobutyl-1-methylxanthine, 0.1 mM) and RO 20–1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone, 0.1 mM) did not abolish the inhibitory effect of sphingosine. To study the mode of action of sphingosine, forskolin and cAMP analogues were tested. The effect of these substances on Steroidogenesis was inhibited, as well as the forskolin-induced cAMP accumulation, by sphingosine.
This study demonstrates a clear inhibition of LH-stimulated effects by sphingosine and psychosine. LH action in rat luteal cells is discussed in relation to protein kinase C and the possible mode of sphingosine action.</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology</subject><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Bucladesine - pharmacology</subject><subject>Colforsin - pharmacology</subject><subject>Corpus Luteum - metabolism</subject><subject>Cyclic AMP</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Cytosol - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Luteal Cells - drug effects</subject><subject>Luteal Cells - metabolism</subject><subject>Luteinizing hormone</subject><subject>Luteinizing Hormone - antagonists & inhibitors</subject><subject>Luteinizing Hormone - pharmacology</subject><subject>Progesterone - biosynthesis</subject><subject>Protein kinase C</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Psychosine</subject><subject>Psychosine - pharmacology</subject><subject>Rat luteal cells</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Sphingosine</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - pharmacology</subject><subject>Steroidogenesis</subject><subject>Vertebrates: endocrinology</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQtRBVWVr-AUi-gEBqih3bsXNBQqt-IK3EAThbXnu8a8g6iydB6r8n6S7trRxG1sx78_T8hpDXnF1yxpuPTDBR6Zrp98Z8aBnXvKqfkQU3uq4MU_o5WTxQXpCXiD8ZY1rV5pSc1rIRrRQL0n3bb1Pe9JgyUJcD3eOd3963FxTHzQZwgEBT3qZ1GvqCtI90X_oBUqa_UnYIdHnxD6erWwoxgh9wGtHiBtqNA7iOeug6PCcn0XUIr47vGflxffV9eVutvt58WX5eVV5yM1RORalapVgTQhRzRe9qwZVTOqx503ilFZOKmxj5uuGi1dIwZwQAN8oEcUbeHXQno7_H6Qd2l3B24DL0I1ptmloozf5L5IpLI1kzEeWB6EuPWCDafUk7V-4sZ3a-hp2jtnPU1hh7fw1bT2tvjvrjegfhYekY_4S_PeIOveticdknfNRudWt0O_v8dODBlNqfBMWiT5A9hFSmsG3o09NG_gIr_qXH</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Sender Baum, Monica G.</creator><creator>Ahrén, Kurt E.B.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19881201</creationdate><title>Sphingosine and psychosine, suggested inhibitors of protein kinase C, inhibit LH effects in rat luteal cells</title><author>Sender Baum, Monica G. ; Ahrén, Kurt E.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-a5f4595506ddf3ddf3fca2315a57db166c57504518ff1b61397480a83ee1858d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology</topic><topic>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Bucladesine - pharmacology</topic><topic>Colforsin - pharmacology</topic><topic>Corpus Luteum - metabolism</topic><topic>Cyclic AMP</topic><topic>Cyclic AMP - biosynthesis</topic><topic>Cytosol - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Luteal Cells - drug effects</topic><topic>Luteal Cells - metabolism</topic><topic>Luteinizing hormone</topic><topic>Luteinizing Hormone - antagonists & inhibitors</topic><topic>Luteinizing Hormone - pharmacology</topic><topic>Progesterone - biosynthesis</topic><topic>Protein kinase C</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Psychosine</topic><topic>Psychosine - pharmacology</topic><topic>Rat luteal cells</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Sphingosine</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - pharmacology</topic><topic>Steroidogenesis</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sender Baum, Monica G.</creatorcontrib><creatorcontrib>Ahrén, Kurt E.B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sender Baum, Monica G.</au><au>Ahrén, Kurt E.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sphingosine and psychosine, suggested inhibitors of protein kinase C, inhibit LH effects in rat luteal cells</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>60</volume><issue>2</issue><spage>127</spage><epage>135</epage><pages>127-135</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><coden>MCEND6</coden><abstract>The possible involvement of protein kinase C on luteinizing hormone (LH) effects in dispersed rat luteal cells was investigated using two substances that have been reported to be protein kinase C inhibitors, sphingosine and psychosine. Sphingosine efficiently inhibited protein kinase C activity both in brain and luteal cytosol fractions. Both substances inhibited LH-stimulated cyclic adenosine monophosphate (cAMP) accumulation in a dose-dependent fashion with an ID
50 at 3–7 μM (sphingosine) and 40 μ.m (psychosine). LH-stimulated progesterone production was also inhibited with an ID
50 at 6–10 μM (sphingosine) and 40–100 μM (psychosine). The inhibition was not due to an increased phosphodiesterase activity since IBMX (3-isobutyl-1-methylxanthine, 0.1 mM) and RO 20–1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone, 0.1 mM) did not abolish the inhibitory effect of sphingosine. To study the mode of action of sphingosine, forskolin and cAMP analogues were tested. The effect of these substances on Steroidogenesis was inhibited, as well as the forskolin-induced cAMP accumulation, by sphingosine.
This study demonstrates a clear inhibition of LH-stimulated effects by sphingosine and psychosine. LH action in rat luteal cells is discussed in relation to protein kinase C and the possible mode of sphingosine action.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>2463943</pmid><doi>10.1016/0303-7207(88)90171-2</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0303-7207 |
| ispartof | Molecular and cellular endocrinology, 1988-12, Vol.60 (2), p.127-135 |
| issn | 0303-7207 1872-8057 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78623570 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 1-Methyl-3-isobutylxanthine - pharmacology 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology 8-Bromo Cyclic Adenosine Monophosphate - pharmacology Animals Biological and medical sciences Brain - drug effects Brain - metabolism Bucladesine - pharmacology Colforsin - pharmacology Corpus Luteum - metabolism Cyclic AMP Cyclic AMP - biosynthesis Cytosol - metabolism Dose-Response Relationship, Drug Female Fundamental and applied biological sciences. Psychology Luteal Cells - drug effects Luteal Cells - metabolism Luteinizing hormone Luteinizing Hormone - antagonists & inhibitors Luteinizing Hormone - pharmacology Progesterone - biosynthesis Protein kinase C Protein Kinase C - antagonists & inhibitors Psychosine Psychosine - pharmacology Rat luteal cells Rats Rats, Inbred Strains Sphingosine Sphingosine - analogs & derivatives Sphingosine - pharmacology Steroidogenesis Vertebrates: endocrinology |
| title | Sphingosine and psychosine, suggested inhibitors of protein kinase C, inhibit LH effects in rat luteal cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T22%3A09%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sphingosine%20and%20psychosine,%20suggested%20inhibitors%20of%20protein%20kinase%20C,%20inhibit%20LH%20effects%20in%20rat%20luteal%20cells&rft.jtitle=Molecular%20and%20cellular%20endocrinology&rft.au=Sender%20Baum,%20Monica%20G.&rft.date=1988-12-01&rft.volume=60&rft.issue=2&rft.spage=127&rft.epage=135&rft.pages=127-135&rft.issn=0303-7207&rft.eissn=1872-8057&rft.coden=MCEND6&rft_id=info:doi/10.1016/0303-7207(88)90171-2&rft_dat=%3Cproquest_cross%3E15148406%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15148406&rft_id=info:pmid/2463943&rft_els_id=0303720788901712&rfr_iscdi=true |