Blockade of voltage-gated outward K + currents of ramified murine microglia by scorpion peptide toxins
Microglial cells were cultured from murine neonatal brain. Ramification of isolated microglia could be induced by the application of astrocyte-conditioned medium (ACM). Voltage-gated outward potassium currents ( I K) were measured in ramified microglial cells 12–24 h after their treatment with ACM....
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| Veröffentlicht in: | Neuroscience letters 1996-11, Vol.219 (1), p.29-32 |
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| creator | Eder, Claudia Klee, Rolf Heinemann, Uwe |
| description | Microglial cells were cultured from murine neonatal brain. Ramification of isolated microglia could be induced by the application of astrocyte-conditioned medium (ACM). Voltage-gated outward potassium currents (
I
K) were measured in ramified microglial cells 12–24 h after their treatment with ACM. The effects of the specific K
+ channel blockers charybdotoxin (CTX), noxiustoxin (NTX) and kaliotoxin (KTX) on
I
K of ramified microglia were studied. All these peptide toxins blocked
I
K in a concentration-dependent manner, while showing a high sensitivity for
I
K. A half-maximal effective concentration (IC
50) of CTX was estimated to be 1.13 nM, while IC
50 values of 1.24 nM and of 0.81 nM were calculated for KTX and for NTX, respectively. In contrast, dendrotoxin (DTX) did not show any effect on
I
K. It is suggested that ramified microglial cells express outward K
+ currents exhibiting pharmacological properties similar that of outward K
+ currents in cytokine-activated ameboid microglia. |
| doi_str_mv | 10.1016/S0304-3940(96)13164-5 |
| format | Article |
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I
K) were measured in ramified microglial cells 12–24 h after their treatment with ACM. The effects of the specific K
+ channel blockers charybdotoxin (CTX), noxiustoxin (NTX) and kaliotoxin (KTX) on
I
K of ramified microglia were studied. All these peptide toxins blocked
I
K in a concentration-dependent manner, while showing a high sensitivity for
I
K. A half-maximal effective concentration (IC
50) of CTX was estimated to be 1.13 nM, while IC
50 values of 1.24 nM and of 0.81 nM were calculated for KTX and for NTX, respectively. In contrast, dendrotoxin (DTX) did not show any effect on
I
K. It is suggested that ramified microglial cells express outward K
+ currents exhibiting pharmacological properties similar that of outward K
+ currents in cytokine-activated ameboid microglia.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(96)13164-5</identifier><identifier>PMID: 8961296</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Charybdotoxin ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Isolated neuron and nerve. Neuroglia ; Kaliotoxin ; Mice ; Mice, Inbred Strains ; Microglia - drug effects ; Noxiustoxin ; Potassium Channels - drug effects ; Potassium current ; Ramified microglia ; Scorpion Venoms - pharmacology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1996-11, Vol.219 (1), p.29-32</ispartof><rights>1996</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-6447062983c058a1a25e56efec459badb3fe25710328416ca5a27fbc9518b9a23</citedby><cites>FETCH-LOGICAL-c389t-6447062983c058a1a25e56efec459badb3fe25710328416ca5a27fbc9518b9a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3940(96)13164-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2492256$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8961296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eder, Claudia</creatorcontrib><creatorcontrib>Klee, Rolf</creatorcontrib><creatorcontrib>Heinemann, Uwe</creatorcontrib><title>Blockade of voltage-gated outward K + currents of ramified murine microglia by scorpion peptide toxins</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Microglial cells were cultured from murine neonatal brain. Ramification of isolated microglia could be induced by the application of astrocyte-conditioned medium (ACM). Voltage-gated outward potassium currents (
I
K) were measured in ramified microglial cells 12–24 h after their treatment with ACM. The effects of the specific K
+ channel blockers charybdotoxin (CTX), noxiustoxin (NTX) and kaliotoxin (KTX) on
I
K of ramified microglia were studied. All these peptide toxins blocked
I
K in a concentration-dependent manner, while showing a high sensitivity for
I
K. A half-maximal effective concentration (IC
50) of CTX was estimated to be 1.13 nM, while IC
50 values of 1.24 nM and of 0.81 nM were calculated for KTX and for NTX, respectively. In contrast, dendrotoxin (DTX) did not show any effect on
I
K. It is suggested that ramified microglial cells express outward K
+ currents exhibiting pharmacological properties similar that of outward K
+ currents in cytokine-activated ameboid microglia.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Charybdotoxin</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Kaliotoxin</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Microglia - drug effects</subject><subject>Noxiustoxin</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium current</subject><subject>Ramified microglia</subject><subject>Scorpion Venoms - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EKtvCT6jkA0IglOLvxCcEVSmolTgAZ2viTFaGJA62U-i_J9td7bWnObzPzLx6CDnn7IIzbt5_Z5KpSlrF3ljzlktuVKWfkA1valHVthZPyeaIPCenOf9ijGmu1Qk5aazhwpoN6T8N0f-GDmns6V0cCmyx2kLBjsal_IXU0Rv6jvolJZxK3lEJxtCHFRiXFCakY_ApbocAtL2n2cc0hzjRGecS1rMl_gtTfkGe9TBkfHmYZ-Tn56sfl1-q22_XXy8_3lZeNrZURqmaGWEb6ZlugIPQqA326JW2LXSt7FHomjMpGsWNBw2i7ltvNW9aC0Kekdf7u3OKfxbMxY0hexwGmDAu2dWN4TU3cgX1Hly755ywd3MKI6R7x5nb-XUPft1OnrPGPfh1et07PzxY2hG749ZB6Jq_OuSQPQx9gsmHfMSEskLoHfZhj-Eq4y5gctkHnDx2IaEvrovhkSL_ASVel0g</recordid><startdate>19961115</startdate><enddate>19961115</enddate><creator>Eder, Claudia</creator><creator>Klee, Rolf</creator><creator>Heinemann, Uwe</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961115</creationdate><title>Blockade of voltage-gated outward K + currents of ramified murine microglia by scorpion peptide toxins</title><author>Eder, Claudia ; Klee, Rolf ; Heinemann, Uwe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-6447062983c058a1a25e56efec459badb3fe25710328416ca5a27fbc9518b9a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Charybdotoxin</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Kaliotoxin</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Microglia - drug effects</topic><topic>Noxiustoxin</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium current</topic><topic>Ramified microglia</topic><topic>Scorpion Venoms - pharmacology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eder, Claudia</creatorcontrib><creatorcontrib>Klee, Rolf</creatorcontrib><creatorcontrib>Heinemann, Uwe</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eder, Claudia</au><au>Klee, Rolf</au><au>Heinemann, Uwe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Blockade of voltage-gated outward K + currents of ramified murine microglia by scorpion peptide toxins</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1996-11-15</date><risdate>1996</risdate><volume>219</volume><issue>1</issue><spage>29</spage><epage>32</epage><pages>29-32</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Microglial cells were cultured from murine neonatal brain. Ramification of isolated microglia could be induced by the application of astrocyte-conditioned medium (ACM). Voltage-gated outward potassium currents (
I
K) were measured in ramified microglial cells 12–24 h after their treatment with ACM. The effects of the specific K
+ channel blockers charybdotoxin (CTX), noxiustoxin (NTX) and kaliotoxin (KTX) on
I
K of ramified microglia were studied. All these peptide toxins blocked
I
K in a concentration-dependent manner, while showing a high sensitivity for
I
K. A half-maximal effective concentration (IC
50) of CTX was estimated to be 1.13 nM, while IC
50 values of 1.24 nM and of 0.81 nM were calculated for KTX and for NTX, respectively. In contrast, dendrotoxin (DTX) did not show any effect on
I
K. It is suggested that ramified microglial cells express outward K
+ currents exhibiting pharmacological properties similar that of outward K
+ currents in cytokine-activated ameboid microglia.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>8961296</pmid><doi>10.1016/S0304-3940(96)13164-5</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1996-11, Vol.219 (1), p.29-32 |
| issn | 0304-3940 1872-7972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78617163 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biological and medical sciences Charybdotoxin Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Isolated neuron and nerve. Neuroglia Kaliotoxin Mice Mice, Inbred Strains Microglia - drug effects Noxiustoxin Potassium Channels - drug effects Potassium current Ramified microglia Scorpion Venoms - pharmacology Vertebrates: nervous system and sense organs |
| title | Blockade of voltage-gated outward K + currents of ramified murine microglia by scorpion peptide toxins |
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