Overexpression of c-myc induces apoptosis at the prophase of meiosis of rat primary spermatocytes
Transgenic rats expressing the rat c‐myc gene under the control of the human metallothionein II A promoter were produced. We found that the female transgenic rats were fertile, but that the male transgenic rats were sterile. Atrophy of the seminiferous tubules and depletion of sperm were observed in...
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| Veröffentlicht in: | Molecular reproduction and development 1996-12, Vol.45 (4), p.403-410 |
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| creator | Kodaira, Kunihiko Takahashi, Ri-Ichi Hirabayashi, Masumi Suzuki, Takashige Obinata, Masuo Ueda, Masatsugu |
| description | Transgenic rats expressing the rat c‐myc gene under the control of the human metallothionein II A promoter were produced. We found that the female transgenic rats were fertile, but that the male transgenic rats were sterile. Atrophy of the seminiferous tubules and depletion of sperm were observed in the sterile male testes. The expression of differential stage‐specific mRNAs, including those of the c‐kit receptor proto‐oncogene, meiotic heat‐shock protein 70 gene, acrosin gene, and transition protein 1 gene, was analyzed by the reverse transcriptase‐polymerase chain reaction during spermatogenesis. The results suggested that spermatogenesis in these sterile rats were arrested at the prophase of meiosis in the primary spermatocytes. We found that apoptotic DNA fragmentation occured in primary spermatocytes of the sterile transgenic rats. These results suggest that overexpression of the c‐myc gene induces apoptosis at the prophase meiosis of the primary spermatocytes thereby causing male sterility in the c‐myc transgenic rats. © Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1098-2795(199612)45:4<403::AID-MRD1>3.0.CO;2-V |
| format | Article |
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We found that the female transgenic rats were fertile, but that the male transgenic rats were sterile. Atrophy of the seminiferous tubules and depletion of sperm were observed in the sterile male testes. The expression of differential stage‐specific mRNAs, including those of the c‐kit receptor proto‐oncogene, meiotic heat‐shock protein 70 gene, acrosin gene, and transition protein 1 gene, was analyzed by the reverse transcriptase‐polymerase chain reaction during spermatogenesis. The results suggested that spermatogenesis in these sterile rats were arrested at the prophase of meiosis in the primary spermatocytes. We found that apoptotic DNA fragmentation occured in primary spermatocytes of the sterile transgenic rats. These results suggest that overexpression of the c‐myc gene induces apoptosis at the prophase meiosis of the primary spermatocytes thereby causing male sterility in the c‐myc transgenic rats. © Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/(SICI)1098-2795(199612)45:4<403::AID-MRD1>3.0.CO;2-V</identifier><identifier>PMID: 8956277</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Animals, Genetically Modified ; apoptosis ; Apoptosis - physiology ; Biological and medical sciences ; c-myc ; DNA Fragmentation ; DNA-Binding Proteins - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Humans ; In Situ Hybridization, Fluorescence ; Infertility, Male - physiopathology ; Male ; Mammalian male genital system ; Meiosis ; Metallothionein - genetics ; Morphology. Physiology ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Prophase ; Proto-Oncogene Proteins c-myc - genetics ; Rats ; RNA, Messenger ; Spermatocytes - physiology ; testis ; Testis - metabolism ; Testis - pathology ; Transcription Factors - genetics ; transgenic rat ; Vertebrates: reproduction ; WT1 Proteins</subject><ispartof>Molecular reproduction and development, 1996-12, Vol.45 (4), p.403-410</ispartof><rights>Copyright © 1996 Wiley‐Liss, Inc.</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c5241-3d038e8b9c288129273ea5ed7b137d8d2cec1e989f2adfbc6db3cdc17ce64b603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291098-2795%28199612%2945%3A4%3C403%3A%3AAID-MRD1%3E3.0.CO%3B2-V$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291098-2795%28199612%2945%3A4%3C403%3A%3AAID-MRD1%3E3.0.CO%3B2-V$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2510651$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8956277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kodaira, Kunihiko</creatorcontrib><creatorcontrib>Takahashi, Ri-Ichi</creatorcontrib><creatorcontrib>Hirabayashi, Masumi</creatorcontrib><creatorcontrib>Suzuki, Takashige</creatorcontrib><creatorcontrib>Obinata, Masuo</creatorcontrib><creatorcontrib>Ueda, Masatsugu</creatorcontrib><title>Overexpression of c-myc induces apoptosis at the prophase of meiosis of rat primary spermatocytes</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>Transgenic rats expressing the rat c‐myc gene under the control of the human metallothionein II A promoter were produced. We found that the female transgenic rats were fertile, but that the male transgenic rats were sterile. Atrophy of the seminiferous tubules and depletion of sperm were observed in the sterile male testes. The expression of differential stage‐specific mRNAs, including those of the c‐kit receptor proto‐oncogene, meiotic heat‐shock protein 70 gene, acrosin gene, and transition protein 1 gene, was analyzed by the reverse transcriptase‐polymerase chain reaction during spermatogenesis. The results suggested that spermatogenesis in these sterile rats were arrested at the prophase of meiosis in the primary spermatocytes. We found that apoptotic DNA fragmentation occured in primary spermatocytes of the sterile transgenic rats. These results suggest that overexpression of the c‐myc gene induces apoptosis at the prophase meiosis of the primary spermatocytes thereby causing male sterility in the c‐myc transgenic rats. © Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Biological and medical sciences</subject><subject>c-myc</subject><subject>DNA Fragmentation</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Infertility, Male - physiopathology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Meiosis</subject><subject>Metallothionein - genetics</subject><subject>Morphology. Physiology</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic</subject><subject>Prophase</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Rats</subject><subject>RNA, Messenger</subject><subject>Spermatocytes - physiology</subject><subject>testis</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Transcription Factors - genetics</subject><subject>transgenic rat</subject><subject>Vertebrates: reproduction</subject><subject>WT1 Proteins</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9v0zAUxSMEGmPwEZDygND2kOK_sV0mpKmFrWjQaRuDtyvHudECTRPsFNZvj7NW5QGkPeVG5_j4-P6S5JiSESWEvTm8mk1mR5QYnTFl5CE1JqfsSMixOBaEj8cns2n26XJK3_ERGU3mb1l28yjZ3x14PMyCZEKyb0-TZyF8J4QYo8lesqeNzJlS-4md_0KPd53HEOp2mbZV6rJm7dJ6Wa4chtR2bde3oY5Tn_a3mHa-7W5twMHaYH0vxdFHufN1Y_06DR36xvatW_cYnidPKrsI-GL7PUi-fHh_PTnLzuens8nJeeYkEzTjJeEadWEc05oywxRHK7FUBeWq1CVz6CgabSpmy6pweVlwVzqqHOaiyAk_SF5vcmPBnysMPTR1cLhY2CW2qwBKx-1pkT9opFIpo6mIxuuN0fk2BI8VbB8IlMCACGBABMPGYdg4bBCBkCAgIgKIiGBABBwITObA4CbGvtzevyoaLHehWyZRf7XVbXB2UXm7dHXY2ZikJJf0b7vf9QLX_1R7oNl_it3_x9hsE1uHHu92sdb_gFxxJeHr51Mw5OLs4-X0Aq74H5bNyYI</recordid><startdate>199612</startdate><enddate>199612</enddate><creator>Kodaira, Kunihiko</creator><creator>Takahashi, Ri-Ichi</creator><creator>Hirabayashi, Masumi</creator><creator>Suzuki, Takashige</creator><creator>Obinata, Masuo</creator><creator>Ueda, Masatsugu</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199612</creationdate><title>Overexpression of c-myc induces apoptosis at the prophase of meiosis of rat primary spermatocytes</title><author>Kodaira, Kunihiko ; Takahashi, Ri-Ichi ; Hirabayashi, Masumi ; Suzuki, Takashige ; Obinata, Masuo ; Ueda, Masatsugu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5241-3d038e8b9c288129273ea5ed7b137d8d2cec1e989f2adfbc6db3cdc17ce64b603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Biological and medical sciences</topic><topic>c-myc</topic><topic>DNA Fragmentation</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Infertility, Male - physiopathology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Meiosis</topic><topic>Metallothionein - genetics</topic><topic>Morphology. Physiology</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic</topic><topic>Prophase</topic><topic>Proto-Oncogene Proteins c-myc - genetics</topic><topic>Rats</topic><topic>RNA, Messenger</topic><topic>Spermatocytes - physiology</topic><topic>testis</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Transcription Factors - genetics</topic><topic>transgenic rat</topic><topic>Vertebrates: reproduction</topic><topic>WT1 Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kodaira, Kunihiko</creatorcontrib><creatorcontrib>Takahashi, Ri-Ichi</creatorcontrib><creatorcontrib>Hirabayashi, Masumi</creatorcontrib><creatorcontrib>Suzuki, Takashige</creatorcontrib><creatorcontrib>Obinata, Masuo</creatorcontrib><creatorcontrib>Ueda, Masatsugu</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kodaira, Kunihiko</au><au>Takahashi, Ri-Ichi</au><au>Hirabayashi, Masumi</au><au>Suzuki, Takashige</au><au>Obinata, Masuo</au><au>Ueda, Masatsugu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of c-myc induces apoptosis at the prophase of meiosis of rat primary spermatocytes</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1996-12</date><risdate>1996</risdate><volume>45</volume><issue>4</issue><spage>403</spage><epage>410</epage><pages>403-410</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>Transgenic rats expressing the rat c‐myc gene under the control of the human metallothionein II A promoter were produced. We found that the female transgenic rats were fertile, but that the male transgenic rats were sterile. Atrophy of the seminiferous tubules and depletion of sperm were observed in the sterile male testes. The expression of differential stage‐specific mRNAs, including those of the c‐kit receptor proto‐oncogene, meiotic heat‐shock protein 70 gene, acrosin gene, and transition protein 1 gene, was analyzed by the reverse transcriptase‐polymerase chain reaction during spermatogenesis. The results suggested that spermatogenesis in these sterile rats were arrested at the prophase of meiosis in the primary spermatocytes. We found that apoptotic DNA fragmentation occured in primary spermatocytes of the sterile transgenic rats. These results suggest that overexpression of the c‐myc gene induces apoptosis at the prophase meiosis of the primary spermatocytes thereby causing male sterility in the c‐myc transgenic rats. © Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8956277</pmid><doi>10.1002/(SICI)1098-2795(199612)45:4<403::AID-MRD1>3.0.CO;2-V</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 1996-12, Vol.45 (4), p.403-410 |
| issn | 1040-452X 1098-2795 |
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| subjects | Animals Animals, Genetically Modified apoptosis Apoptosis - physiology Biological and medical sciences c-myc DNA Fragmentation DNA-Binding Proteins - genetics Female Fundamental and applied biological sciences. Psychology Gene Expression Humans In Situ Hybridization, Fluorescence Infertility, Male - physiopathology Male Mammalian male genital system Meiosis Metallothionein - genetics Morphology. Physiology Polymerase Chain Reaction Promoter Regions, Genetic Prophase Proto-Oncogene Proteins c-myc - genetics Rats RNA, Messenger Spermatocytes - physiology testis Testis - metabolism Testis - pathology Transcription Factors - genetics transgenic rat Vertebrates: reproduction WT1 Proteins |
| title | Overexpression of c-myc induces apoptosis at the prophase of meiosis of rat primary spermatocytes |
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