Quantitation of FAD-Dependent Cytochrome P450 Reductase Activity by Photoreduction

NADPH cytochrome P450 reductase binds two flavin cofactors, FMN and FAD, per molecule of reductase. We have developed an assay to quantitate the reduction activity of FMN-bound flavoprotein. This Tris–light assay system takes advantage of the ability of photoactivated flavins to release electrons to...

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Veröffentlicht in:	Analytical biochemistry 1996-12, Vol.243 (1), p.154-157
Hauptverfasser:	Hodgson, Anne V., Strobel, Henry W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System - metabolism Flavin Mononucleotide - metabolism Flavin-Adenine Dinucleotide - metabolism Mixed Function Oxygenases - metabolism NADP - metabolism NADPH-Ferrihemoprotein Reductase - metabolism Superoxide Dismutase - metabolism
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