A Heterologous Assay for Measuring Prolactin in Pituitary Extracts and Plasma from Australian Flying Foxes (GenusPteropus)
A sensitive heterologous assay was developed to measure prolactin-like activity inPteropus alecto, P. poliocephalus,andP. scapulatus,Australian flying foxes. Adapted from an established radioimmunoassay for rabbit PRL, it utilises the well-characterised, polyclonal antiserum 33/9 (guinea pig anti-hu...
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| Veröffentlicht in: | General and comparative endocrinology 1996-12, Vol.104 (3), p.304-311 |
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| creator | O'Brien, G.M. Curlewis, J.D. Martin, L. |
| description | A sensitive heterologous assay was developed to measure prolactin-like activity inPteropus alecto, P. poliocephalus,andP. scapulatus,Australian flying foxes. Adapted from an established radioimmunoassay for rabbit PRL, it utilises the well-characterised, polyclonal antiserum 33/9 (guinea pig anti-human prolactin). In the assay, pituitary extracts fromP. alecto, P. poliocephalus,andP. scapulatusdiluted in parallel with ovine prolactin standards, although absolute levels estimated were low. Its usefulness for investigating the role of prolactin in reproduction and seasonality in flying foxes was tested. In a survey of pituitary extracts collected from both sexes of all three species, prolactin was higher in females than in males (P |
| doi_str_mv | 10.1006/gcen.1996.0175 |
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Adapted from an established radioimmunoassay for rabbit PRL, it utilises the well-characterised, polyclonal antiserum 33/9 (guinea pig anti-human prolactin). In the assay, pituitary extracts fromP. alecto, P. poliocephalus,andP. scapulatusdiluted in parallel with ovine prolactin standards, although absolute levels estimated were low. Its usefulness for investigating the role of prolactin in reproduction and seasonality in flying foxes was tested. In a survey of pituitary extracts collected from both sexes of all three species, prolactin was higher in females than in males (P<0.001). In the few specimens from juveniles, mean prolactin levels in pituitary and plasma were similar to those of adults. Plasma and pituitary samples both contained higher concentrations of prolactin during late pregnancy (P. scapulatus,plasmaP<0.01; pituitaryP<0.01) and lactation (P. poliocephalus,plasmaP<0.005; pituitaryP<0.05) in mature females. Plasma prolactin increased at about the time of parturition, but returned to nonpregnant levels rapidly if lactation was not established. In lactating females, plasma prolactin was suppressed by temporary removal of the sucking young, and was slow to recover after the young was returned to the nipple. Pharmacological responses were tested in pregnantP. poliocephalus:plasma prolactin was low following bromocriptine administration and elevated following domperidone. Prolactin is concluded to play significant roles in the reproductive physiology of female flying foxes and, as in other species, is under dopaminergic regulation.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1006/gcen.1996.0175</identifier><identifier>PMID: 8954763</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bromocriptine - pharmacology ; Chiroptera - metabolism ; Domperidone - pharmacology ; Dopamine Agonists - pharmacology ; Dopamine Antagonists - pharmacology ; Female ; Lactation - physiology ; Male ; Pituitary Gland - chemistry ; Pregnancy ; Prolactin - analysis ; Prolactin - blood ; Radioimmunoassay - methods ; Reproduction - physiology ; Seasons ; Tissue Extracts - chemistry</subject><ispartof>General and comparative endocrinology, 1996-12, Vol.104 (3), p.304-311</ispartof><rights>1996 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-cd8131c7cbca1b846985224b16d804e26b46dba2b85fd1abf292e519a88ce0e43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016648096901756$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8954763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Brien, G.M.</creatorcontrib><creatorcontrib>Curlewis, J.D.</creatorcontrib><creatorcontrib>Martin, L.</creatorcontrib><title>A Heterologous Assay for Measuring Prolactin in Pituitary Extracts and Plasma from Australian Flying Foxes (GenusPteropus)</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>A sensitive heterologous assay was developed to measure prolactin-like activity inPteropus alecto, P. poliocephalus,andP. scapulatus,Australian flying foxes. Adapted from an established radioimmunoassay for rabbit PRL, it utilises the well-characterised, polyclonal antiserum 33/9 (guinea pig anti-human prolactin). In the assay, pituitary extracts fromP. alecto, P. poliocephalus,andP. scapulatusdiluted in parallel with ovine prolactin standards, although absolute levels estimated were low. Its usefulness for investigating the role of prolactin in reproduction and seasonality in flying foxes was tested. In a survey of pituitary extracts collected from both sexes of all three species, prolactin was higher in females than in males (P<0.001). In the few specimens from juveniles, mean prolactin levels in pituitary and plasma were similar to those of adults. Plasma and pituitary samples both contained higher concentrations of prolactin during late pregnancy (P. scapulatus,plasmaP<0.01; pituitaryP<0.01) and lactation (P. poliocephalus,plasmaP<0.005; pituitaryP<0.05) in mature females. Plasma prolactin increased at about the time of parturition, but returned to nonpregnant levels rapidly if lactation was not established. In lactating females, plasma prolactin was suppressed by temporary removal of the sucking young, and was slow to recover after the young was returned to the nipple. Pharmacological responses were tested in pregnantP. poliocephalus:plasma prolactin was low following bromocriptine administration and elevated following domperidone. Prolactin is concluded to play significant roles in the reproductive physiology of female flying foxes and, as in other species, is under dopaminergic regulation.</description><subject>Animals</subject><subject>Bromocriptine - pharmacology</subject><subject>Chiroptera - metabolism</subject><subject>Domperidone - pharmacology</subject><subject>Dopamine Agonists - pharmacology</subject><subject>Dopamine Antagonists - pharmacology</subject><subject>Female</subject><subject>Lactation - physiology</subject><subject>Male</subject><subject>Pituitary Gland - chemistry</subject><subject>Pregnancy</subject><subject>Prolactin - analysis</subject><subject>Prolactin - blood</subject><subject>Radioimmunoassay - methods</subject><subject>Reproduction - physiology</subject><subject>Seasons</subject><subject>Tissue Extracts - chemistry</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LxDAQxYMoun5cvQk5iR66Jm2aTY_L4qqguAc9hzSdLpG2WTOtuP71puziTQgM5L15w_sRcsnZlDMm79YWuikvCjllfJYfkAlnRZ5IJdghmTDGZSKFYifkFPGDMZZnkh-TY1XkYiazCfmZ00foIfjGr_2AdI5otrT2gb6AwSG4bk1XUTW2dx2Nb-X6wfUmbOn9dx_iN1LTVXTVGGwNrYNv6XzAqDTOdHTZbMeEpf8GpDcP0A24Gq9tBrw9J0e1aRAu9vOMvC_v3xaPyfPrw9Ni_pxYkYk-sZXiGbczW1rDSyVkofI0FSWXlWICUlkKWZUmLVVeV9yUdVqkkPPCKGWBgcjOyPUudxP85wDY69ahhaYxHcTKeqYkTzMuo3G6M9rgEQPUehNcG6tqzvQIW4-w9Qhbj7DjwtU-eShbqP7se7pRVzsdYr0vB0GjddBZqFwA2-vKu_-ifwG-jI_1</recordid><startdate>19961201</startdate><enddate>19961201</enddate><creator>O'Brien, G.M.</creator><creator>Curlewis, J.D.</creator><creator>Martin, L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961201</creationdate><title>A Heterologous Assay for Measuring Prolactin in Pituitary Extracts and Plasma from Australian Flying Foxes (GenusPteropus)</title><author>O'Brien, G.M. ; Curlewis, J.D. ; Martin, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-cd8131c7cbca1b846985224b16d804e26b46dba2b85fd1abf292e519a88ce0e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Bromocriptine - pharmacology</topic><topic>Chiroptera - metabolism</topic><topic>Domperidone - pharmacology</topic><topic>Dopamine Agonists - pharmacology</topic><topic>Dopamine Antagonists - pharmacology</topic><topic>Female</topic><topic>Lactation - physiology</topic><topic>Male</topic><topic>Pituitary Gland - chemistry</topic><topic>Pregnancy</topic><topic>Prolactin - analysis</topic><topic>Prolactin - blood</topic><topic>Radioimmunoassay - methods</topic><topic>Reproduction - physiology</topic><topic>Seasons</topic><topic>Tissue Extracts - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Brien, G.M.</creatorcontrib><creatorcontrib>Curlewis, J.D.</creatorcontrib><creatorcontrib>Martin, L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Brien, G.M.</au><au>Curlewis, J.D.</au><au>Martin, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Heterologous Assay for Measuring Prolactin in Pituitary Extracts and Plasma from Australian Flying Foxes (GenusPteropus)</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1996-12-01</date><risdate>1996</risdate><volume>104</volume><issue>3</issue><spage>304</spage><epage>311</epage><pages>304-311</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><abstract>A sensitive heterologous assay was developed to measure prolactin-like activity inPteropus alecto, P. poliocephalus,andP. scapulatus,Australian flying foxes. Adapted from an established radioimmunoassay for rabbit PRL, it utilises the well-characterised, polyclonal antiserum 33/9 (guinea pig anti-human prolactin). In the assay, pituitary extracts fromP. alecto, P. poliocephalus,andP. scapulatusdiluted in parallel with ovine prolactin standards, although absolute levels estimated were low. Its usefulness for investigating the role of prolactin in reproduction and seasonality in flying foxes was tested. In a survey of pituitary extracts collected from both sexes of all three species, prolactin was higher in females than in males (P<0.001). In the few specimens from juveniles, mean prolactin levels in pituitary and plasma were similar to those of adults. Plasma and pituitary samples both contained higher concentrations of prolactin during late pregnancy (P. scapulatus,plasmaP<0.01; pituitaryP<0.01) and lactation (P. poliocephalus,plasmaP<0.005; pituitaryP<0.05) in mature females. Plasma prolactin increased at about the time of parturition, but returned to nonpregnant levels rapidly if lactation was not established. In lactating females, plasma prolactin was suppressed by temporary removal of the sucking young, and was slow to recover after the young was returned to the nipple. Pharmacological responses were tested in pregnantP. poliocephalus:plasma prolactin was low following bromocriptine administration and elevated following domperidone. Prolactin is concluded to play significant roles in the reproductive physiology of female flying foxes and, as in other species, is under dopaminergic regulation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8954763</pmid><doi>10.1006/gcen.1996.0175</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bromocriptine - pharmacology Chiroptera - metabolism Domperidone - pharmacology Dopamine Agonists - pharmacology Dopamine Antagonists - pharmacology Female Lactation - physiology Male Pituitary Gland - chemistry Pregnancy Prolactin - analysis Prolactin - blood Radioimmunoassay - methods Reproduction - physiology Seasons Tissue Extracts - chemistry |
| title | A Heterologous Assay for Measuring Prolactin in Pituitary Extracts and Plasma from Australian Flying Foxes (GenusPteropus) |
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