Capturing the Structure of a Catalytic RNA Intermediate: The Hammerhead Ribozyme
The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modifie...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1996-12, Vol.274 (5295), p.2065-2069 |
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| container_issue | 5295 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Scott, William G. Murray, James B. John R. P. Arnold Stoddard, Barry L. Klug, Aaron |
| description | The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state. |
| doi_str_mv | 10.1126/science.274.5295.2065 |
| format | Article |
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The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Catalytic RNA</subject><subject>Chemical bases</subject><subject>Crystal structure</subject><subject>Crystallization</subject><subject>Crystallography, X-Ray</subject><subject>Crystals</subject><subject>Data Collection</subject><subject>Enzymes</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. 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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1996-12, Vol.274 (5295), p.2065-2069 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78609791 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Catalytic RNA Chemical bases Crystal structure Crystallization Crystallography, X-Ray Crystals Data Collection Enzymes Freezing Fundamental and applied biological sciences. Psychology Hydrogen bonds Hydrogen-Ion Concentration Ions Literary Devices Magnesium - metabolism Manganese - metabolism Metal ions Models, Molecular Nucleic Acid Conformation Nucleic acids Oxygen Phosphates Physiological aspects Ribonucleic acid RNA RNA, Catalytic - chemistry RNA, Catalytic - metabolism Rna, ribonucleoproteins Scientific Concepts |
| title | Capturing the Structure of a Catalytic RNA Intermediate: The Hammerhead Ribozyme |
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