Effects of Proximate Cholesterol Precursors and Steroid Hormones on Mouse Myeloma Growth in Serum-Free Medium
The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert$[^{3}H]lathosterol$to$[^{3}H]cholesterol$. These results are consist...
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| Veröffentlicht in: | In Vitro Cellular & Developmental Biology 1988-12, Vol.24 (12), p.1223-1228 |
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| container_title | In Vitro Cellular & Developmental Biology |
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| creator | Sato, J. Denry Hui-Ting Cao Kayada, Yoshiaki Cabot, Myles C. Sato, Gordon H. Okamoto, Tetsuji Clement J. Welsh |
| description | The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert$[^{3}H]lathosterol$to$[^{3}H]cholesterol$. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesterone and hydrocortisone could not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use of sterols in defined culture medium for mouse myeloma cells. |
| doi_str_mv | 10.1007/bf02624194 |
| format | Article |
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Denry ; Hui-Ting Cao ; Kayada, Yoshiaki ; Cabot, Myles C. ; Sato, Gordon H. ; Okamoto, Tetsuji ; Clement J. Welsh</creator><creatorcontrib>Sato, J. Denry ; Hui-Ting Cao ; Kayada, Yoshiaki ; Cabot, Myles C. ; Sato, Gordon H. ; Okamoto, Tetsuji ; Clement J. Welsh</creatorcontrib><description>The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert$[^{3}H]lathosterol$to$[^{3}H]cholesterol$. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesterone and hydrocortisone could not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use of sterols in defined culture medium for mouse myeloma cells.</description><identifier>ISSN: 0883-8364</identifier><identifier>EISSN: 2327-431X</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/bf02624194</identifier><identifier>PMID: 3209588</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>3-Hydroxysteroid Dehydrogenases - metabolism ; Animal cells ; Animals ; Biological and medical sciences ; Biotechnology ; Cell cultures. Hybridization. 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Denry</creatorcontrib><creatorcontrib>Hui-Ting Cao</creatorcontrib><creatorcontrib>Kayada, Yoshiaki</creatorcontrib><creatorcontrib>Cabot, Myles C.</creatorcontrib><creatorcontrib>Sato, Gordon H.</creatorcontrib><creatorcontrib>Okamoto, Tetsuji</creatorcontrib><creatorcontrib>Clement J. Welsh</creatorcontrib><title>Effects of Proximate Cholesterol Precursors and Steroid Hormones on Mouse Myeloma Growth in Serum-Free Medium</title><title>In Vitro Cellular & Developmental Biology</title><addtitle>In Vitro Cell Dev Biol</addtitle><description>The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert$[^{3}H]lathosterol$to$[^{3}H]cholesterol$. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesterone and hydrocortisone could not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use of sterols in defined culture medium for mouse myeloma cells.</description><subject>3-Hydroxysteroid Dehydrogenases - metabolism</subject><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell lines</subject><subject>Cellular metabolism</subject><subject>Cholestadienols - pharmacology</subject><subject>Cholesterol - metabolism</subject><subject>Cholesterol - pharmacology</subject><subject>Cholesterols</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Dehydrocholesterols - pharmacology</subject><subject>Desmosterol - pharmacology</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones - pharmacology</subject><subject>Hydrocortisone - pharmacology</subject><subject>Isomerism</subject><subject>L cells</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Multiple Myeloma - metabolism</subject><subject>Multiple Myeloma - pathology</subject><subject>Progesterone - pharmacology</subject><subject>Rapid Communications in Cell Biology</subject><subject>Steroid hormones</subject><subject>Sterols</subject><subject>Testosterone - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0883-8364</issn><issn>2327-431X</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtr3DAUhUVoSSePTdctaBGyKDjV-7Fsh0xSyJDCZJGdkeUr4mBbqWST5N9Hw0xndeGc7x7uPQh9peSKEqJ_NoEwxQS14ggtGGe6Epw-fkILYgyvDFfiCzrJ-ZkQThRjx-iYM2KlMQs0XIcAfso4Bvw3xbducBPg5VPsIU-QYl9U8HPKMWXsxhZvtmrX4tuYhjhCWRzxOs4Z8Pod-jg4fJPi6_SEuxFvIM1DtUpQTGi7eThDn4PrM5zv5yl6WF0_LG-ru_ubP8tfd5Xn1kwVIyFY3rYitN41DqQEwYN0tvWNNEyJABo4VaKRVkpitNJWe8YVp9p6yk_R5S72JcV_c3mkHrrsoe_dCOXUWhtFNJGsgD92oE8x5wShfkmlgfReU1Jvq61_r_5XW-Dv-9S5GaA9oPsui3-x9132rg_Jjb7LB0wZyinZxnzbYc95iulgC2YV14Z_ACmkikM</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Sato, J. Denry</creator><creator>Hui-Ting Cao</creator><creator>Kayada, Yoshiaki</creator><creator>Cabot, Myles C.</creator><creator>Sato, Gordon H.</creator><creator>Okamoto, Tetsuji</creator><creator>Clement J. Welsh</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19881201</creationdate><title>Effects of Proximate Cholesterol Precursors and Steroid Hormones on Mouse Myeloma Growth in Serum-Free Medium</title><author>Sato, J. Denry ; Hui-Ting Cao ; Kayada, Yoshiaki ; Cabot, Myles C. ; Sato, Gordon H. ; Okamoto, Tetsuji ; Clement J. Welsh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-20ff93dd4fdcabae55e43f5a9dcb58264fe7e3164b59550876797c2363179c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>3-Hydroxysteroid Dehydrogenases - metabolism</topic><topic>Animal cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell lines</topic><topic>Cellular metabolism</topic><topic>Cholestadienols - pharmacology</topic><topic>Cholesterol - metabolism</topic><topic>Cholesterol - pharmacology</topic><topic>Cholesterols</topic><topic>Culture Media</topic><topic>Cultured cells</topic><topic>Dehydrocholesterols - pharmacology</topic><topic>Desmosterol - pharmacology</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones - pharmacology</topic><topic>Hydrocortisone - pharmacology</topic><topic>Isomerism</topic><topic>L cells</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Multiple Myeloma - metabolism</topic><topic>Multiple Myeloma - pathology</topic><topic>Progesterone - pharmacology</topic><topic>Rapid Communications in Cell Biology</topic><topic>Steroid hormones</topic><topic>Sterols</topic><topic>Testosterone - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, J. Denry</creatorcontrib><creatorcontrib>Hui-Ting Cao</creatorcontrib><creatorcontrib>Kayada, Yoshiaki</creatorcontrib><creatorcontrib>Cabot, Myles C.</creatorcontrib><creatorcontrib>Sato, Gordon H.</creatorcontrib><creatorcontrib>Okamoto, Tetsuji</creatorcontrib><creatorcontrib>Clement J. 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Welsh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Proximate Cholesterol Precursors and Steroid Hormones on Mouse Myeloma Growth in Serum-Free Medium</atitle><jtitle>In Vitro Cellular & Developmental Biology</jtitle><addtitle>In Vitro Cell Dev Biol</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>24</volume><issue>12</issue><spage>1223</spage><epage>1228</epage><pages>1223-1228</pages><issn>0883-8364</issn><eissn>2327-431X</eissn><eissn>1475-2689</eissn><coden>ICDBEO</coden><abstract>The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert$[^{3}H]lathosterol$to$[^{3}H]cholesterol$. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesterone and hydrocortisone could not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use of sterols in defined culture medium for mouse myeloma cells.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>3209588</pmid><doi>10.1007/bf02624194</doi><tpages>6</tpages></addata></record> |
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| subjects | 3-Hydroxysteroid Dehydrogenases - metabolism Animal cells Animals Biological and medical sciences Biotechnology Cell cultures. Hybridization. Fusion Cell Division - drug effects Cell growth Cell lines Cellular metabolism Cholestadienols - pharmacology Cholesterol - metabolism Cholesterol - pharmacology Cholesterols Culture Media Cultured cells Dehydrocholesterols - pharmacology Desmosterol - pharmacology Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Hormones - pharmacology Hydrocortisone - pharmacology Isomerism L cells Methods. Procedures. Technologies Mice Molecular and cellular biology Multiple Myeloma - metabolism Multiple Myeloma - pathology Progesterone - pharmacology Rapid Communications in Cell Biology Steroid hormones Sterols Testosterone - pharmacology Tumor Cells, Cultured |
| title | Effects of Proximate Cholesterol Precursors and Steroid Hormones on Mouse Myeloma Growth in Serum-Free Medium |
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