Low‐density‐lipoprotein‐receptor mRNA content of fibroblasts from normal and familial hypercholesterolaemic subjects

The amount of mRNA for the low‐density‐lipoprotein (LDL) receptor in cultured human fibroblasts was estimated by hybridization of the poly(A)‐rich RNA fraction with a DNA probe, using the recovery of β‐actin mRNA to correct for losses. During incubation of the cells with lipoprotein‐deficient serum...

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Veröffentlicht in:	European journal of biochemistry 1988-12, Vol.178 (2), p.555-561
Hauptverfasser:	KNIGHT, Brian L., PATEL, Dilip D., GAVIGAN, Sean J. P., SOUTAR, Anne K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Cells, Cultured Chromatography - methods DNA Probes Electrophoresis - methods Fibroblasts - analysis Fundamental and applied biological sciences. Psychology Homozygote Humans Hyperlipoproteinemia Type II - genetics Hyperlipoproteinemia Type II - metabolism Lymphocytes - analysis Nucleic Acid Hybridization Other biological molecules Poly A - analysis Receptors, LDL - analysis Receptors, LDL - biosynthesis RNA Probes RNA, Messenger - analysis Terpenes, steroids. Hormones
Online-Zugang:	Volltext
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