Gap junctions in the adult cerebral cortex: Regional differences in their distribution and cellular expression of connexins

Gap junctions are membrane channels that mediate electrical and metabolic coupling between adjacent cells. Immunocytochemical analysis by using a panel of anti‐connexin antibodies, as well as electron microscopy of thin sections and freeze‐fracture replicas, has shown that gap junctions and their co...

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Veröffentlicht in:Journal of comparative neurology (1911) 1996-12, Vol.376 (2), p.326-342
Hauptverfasser: Nadarajah, B., Thomaidou, D., Evans, W.H., Parnavelas, J.G.
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container_title Journal of comparative neurology (1911)
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creator Nadarajah, B.
Thomaidou, D.
Evans, W.H.
Parnavelas, J.G.
description Gap junctions are membrane channels that mediate electrical and metabolic coupling between adjacent cells. Immunocytochemical analysis by using a panel of anti‐connexin antibodies, as well as electron microscopy of thin sections and freeze‐fracture replicas, has shown that gap junctions and their constituent proteins are abundant in the cerebral cortex of the adult rat. Their frequency and distribution vary in different cortical regions, which may reflect differences in the cellular and functional organization of these areas of the cortex. Gap junctions were identified between glial cells and, less frequently, between neuronal elements. Heterologous junctions were also identified between astrocytes and oligodendrocytes and between neurons and glia; the latter category included abundant junctions between astrocytic processes and neurons. Double‐antibody labelling experiments in tissue sections and in acutely dissociated cells showed that connexin 32 was expressed in neurons and oligodendrocytes, whereas connexin 43, widely believed to be expressed only in astrocytes, was also localized in a population of cortical neurons. These results show that gap junctions can provide a major nonsynaptic means of communication between cortical cell types. © 1996 Wiley‐Liss, Inc.
doi_str_mv 10.1002/(SICI)1096-9861(19961209)376:2<326::AID-CNE13>3.0.CO;2-J
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Double‐antibody labelling experiments in tissue sections and in acutely dissociated cells showed that connexin 32 was expressed in neurons and oligodendrocytes, whereas connexin 43, widely believed to be expressed only in astrocytes, was also localized in a population of cortical neurons. 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Comp. Neurol</addtitle><description>Gap junctions are membrane channels that mediate electrical and metabolic coupling between adjacent cells. Immunocytochemical analysis by using a panel of anti‐connexin antibodies, as well as electron microscopy of thin sections and freeze‐fracture replicas, has shown that gap junctions and their constituent proteins are abundant in the cerebral cortex of the adult rat. Their frequency and distribution vary in different cortical regions, which may reflect differences in the cellular and functional organization of these areas of the cortex. Gap junctions were identified between glial cells and, less frequently, between neuronal elements. Heterologous junctions were also identified between astrocytes and oligodendrocytes and between neurons and glia; the latter category included abundant junctions between astrocytic processes and neurons. 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source Wiley Online Library - AutoHoldings Journals; MEDLINE
subjects Animals
Astrocytes - ultrastructure
Blotting, Western
Cell Communication
Cerebral Cortex - chemistry
Cerebral Cortex - ultrastructure
connexin immunocytochemistry
Connexins - analysis
electron microscopy
freeze fracture
Gap Junctions - chemistry
Gap Junctions - ultrastructure
intercellular junctions
Microscopy, Electron
neocortex
Nerve Tissue Proteins - analysis
Neurons - ultrastructure
Oligodendroglia - ultrastructure
Organ Specificity
Rats
Rats, Sprague-Dawley
title Gap junctions in the adult cerebral cortex: Regional differences in their distribution and cellular expression of connexins
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