Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor
Since most of our knowledge on pharmacological properties of brain 5-HT4 receptors have been discussed for mouse colliculi neurons, we cloned the corresponding receptor using the RT-PCR approach. As expected, the homology with the already cloned rat 5-HT(4L) receptor was high, revealing only 16 diff...
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| Veröffentlicht in: | FEBS letters 1996-11, Vol.398 (1), p.19-25 |
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| creator | Claeysen, S Sebben, M Journot, L Bockaert, J Dumuis, A |
| description | Since most of our knowledge on pharmacological properties of brain 5-HT4 receptors have been discussed for mouse colliculi neurons, we cloned the corresponding receptor using the RT-PCR approach. As expected, the homology with the already cloned rat 5-HT(4L) receptor was high, revealing only 16 differences at the amino-acid level. One of the differences, proline75 in mouse, alanine75 in the already published rat sequences was not confirmed. Therefore this proline is part of the consensus sequence present in all 5-HT receptor transmembrane domain II (LVMP). Comparing the affinities of 11 agonists and five antagonists for the cloned mouse receptor (5-HT(4L))expressed in LLCPK1 and the corresponding receptor in mouse colliculi shows an excellent correlation. The transfected mouse 5-HT(4L) receptor stimulated cAMP production. When expressed at high density, it exhibited intrinsic activity. In contrast to the previously described distribution, we found that mRNA encoding for both the short (5-HT(4S))and the long form (5-HT(4L)) of 5-HT4 receptors are expressed in all mouse and rat brain areas. |
| doi_str_mv | 10.1016/s0014-5793(96)01132-5 |
| format | Article |
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As expected, the homology with the already cloned rat 5-HT(4L) receptor was high, revealing only 16 differences at the amino-acid level. One of the differences, proline75 in mouse, alanine75 in the already published rat sequences was not confirmed. Therefore this proline is part of the consensus sequence present in all 5-HT receptor transmembrane domain II (LVMP). Comparing the affinities of 11 agonists and five antagonists for the cloned mouse receptor (5-HT(4L))expressed in LLCPK1 and the corresponding receptor in mouse colliculi shows an excellent correlation. The transfected mouse 5-HT(4L) receptor stimulated cAMP production. When expressed at high density, it exhibited intrinsic activity. 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In contrast to the previously described distribution, we found that mRNA encoding for both the short (5-HT(4S))and the long form (5-HT(4L)) of 5-HT4 receptors are expressed in all mouse and rat brain areas.</description><subject>Age Factors</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Brain - anatomy & histology</subject><subject>Brain - growth & development</subject><subject>Brain - metabolism</subject><subject>Brain Chemistry</subject><subject>Cloning, Molecular</subject><subject>Inferior Colliculi - chemistry</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Serotonin - drug effects</subject><subject>Receptors, Serotonin - genetics</subject><subject>Receptors, Serotonin - metabolism</subject><subject>Receptors, Serotonin, 5-HT4</subject><subject>Serotonin Antagonists - pharmacology</subject><subject>Serotonin Receptor Agonists - pharmacology</subject><subject>Species Specificity</subject><subject>Transfection</subject><issn>0014-5793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9T11rgzAUzcNG13X7CYU8jRbmlhij5nFItw6EvfguV3PTWtS4RGH99xMmgwuH88HhXEK2nL1wxuNXzxiPApkosVPxnnEuwkDekPW_fEfuvb-wmadcrcgqVVE835pkWWv7pj89U_wZHHrf2J5Cr-lwBtdBbVt7ulJr6HhG2tnJI5XBsdhF-Z46rHEYrXsgtwZaj48LbkjxfiiyY5B_fXxmb3lwCXk8BoqDBgRt4gR0nWglKomRqCJQqOJUCl1XOuQStOBaJ8yI2TPGcFEBAxQb8vRXOzj7PaEfy67xNbYt9DgPK5NUpqGI5BzcLsGp6lCXg2s6cNdyeVr8AqQ1WIc</recordid><startdate>19961125</startdate><enddate>19961125</enddate><creator>Claeysen, S</creator><creator>Sebben, M</creator><creator>Journot, L</creator><creator>Bockaert, J</creator><creator>Dumuis, A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19961125</creationdate><title>Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor</title><author>Claeysen, S ; Sebben, M ; Journot, L ; Bockaert, J ; Dumuis, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j216t-91adaeadf67adc7d93b5e43b4a9e96853dcbd215ad31dd70f33b4fff13ba0ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Age Factors</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Brain - anatomy & histology</topic><topic>Brain - growth & development</topic><topic>Brain - metabolism</topic><topic>Brain Chemistry</topic><topic>Cloning, Molecular</topic><topic>Inferior Colliculi - chemistry</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Serotonin - drug effects</topic><topic>Receptors, Serotonin - genetics</topic><topic>Receptors, Serotonin - metabolism</topic><topic>Receptors, Serotonin, 5-HT4</topic><topic>Serotonin Antagonists - pharmacology</topic><topic>Serotonin Receptor Agonists - pharmacology</topic><topic>Species Specificity</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Claeysen, S</creatorcontrib><creatorcontrib>Sebben, M</creatorcontrib><creatorcontrib>Journot, L</creatorcontrib><creatorcontrib>Bockaert, J</creatorcontrib><creatorcontrib>Dumuis, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Claeysen, S</au><au>Sebben, M</au><au>Journot, L</au><au>Bockaert, J</au><au>Dumuis, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-11-25</date><risdate>1996</risdate><volume>398</volume><issue>1</issue><spage>19</spage><epage>25</epage><pages>19-25</pages><issn>0014-5793</issn><abstract>Since most of our knowledge on pharmacological properties of brain 5-HT4 receptors have been discussed for mouse colliculi neurons, we cloned the corresponding receptor using the RT-PCR approach. As expected, the homology with the already cloned rat 5-HT(4L) receptor was high, revealing only 16 differences at the amino-acid level. One of the differences, proline75 in mouse, alanine75 in the already published rat sequences was not confirmed. Therefore this proline is part of the consensus sequence present in all 5-HT receptor transmembrane domain II (LVMP). Comparing the affinities of 11 agonists and five antagonists for the cloned mouse receptor (5-HT(4L))expressed in LLCPK1 and the corresponding receptor in mouse colliculi shows an excellent correlation. The transfected mouse 5-HT(4L) receptor stimulated cAMP production. When expressed at high density, it exhibited intrinsic activity. In contrast to the previously described distribution, we found that mRNA encoding for both the short (5-HT(4S))and the long form (5-HT(4L)) of 5-HT4 receptors are expressed in all mouse and rat brain areas.</abstract><cop>England</cop><pmid>8946946</pmid><doi>10.1016/s0014-5793(96)01132-5</doi><tpages>7</tpages></addata></record> |
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| ispartof | FEBS letters, 1996-11, Vol.398 (1), p.19-25 |
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| source | MEDLINE; Wiley Journals; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Age Factors Amino Acid Sequence Animals Animals, Newborn Base Sequence Binding Sites Brain - anatomy & histology Brain - growth & development Brain - metabolism Brain Chemistry Cloning, Molecular Inferior Colliculi - chemistry Mice Molecular Sequence Data Rats Rats, Inbred Strains Receptors, Serotonin - drug effects Receptors, Serotonin - genetics Receptors, Serotonin - metabolism Receptors, Serotonin, 5-HT4 Serotonin Antagonists - pharmacology Serotonin Receptor Agonists - pharmacology Species Specificity Transfection |
| title | Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor |
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